Journal of Neurophysiology, Vol 58, Issue 3 598-613, Copyright © 1987 by APS

ARTICLES

Effects of physiological amounts of high- and low-rate chronic stimulation on fast-twitch muscle of the cat hindlimb. I. Speed- and force-related properties

D. Kernell, O. Eerbeek, B. A. Verhey and Y. Donselaar
Department of Neurophysiology, University of Amsterdam, Academisch Medisch Centrum, Netherlands.

1. Long-term electrical stimulation was given during 4 or 8 wk to the peroneal nerve of deafferented hindlimbs in hemispinalized adult cats. Four different stimulation patterns were compared: 100-Hz bursts covering 5% of daily time (F1), 10-Hz bursts covering 5% of daily time (S1), pattern S1 plus added 100-Hz bursts during 0.5% of daily time (S1F2), and, finally, only the latter 100-Hz bursts (F2), again during 0.5% of daily time. 2. During the course of chronic stimulation, frequent noninvasive measurements were made of the twitch of the ankle dorsiflexors. In a terminal acute experiment under general anesthesia, performed after 4 or 8 wk of treatment, measurements were made of isometric contractile properties (speed, force) for one of the stimulated peroneal muscles, m. peroneus longus (PerL). Thereafter, the PerL muscle was removed for further histochemical/histological analysis. 3. Findings from chronically stimulated PerL muscles were compared with three kinds of control PerL muscles: 1) those from the contralateral (control) hindlimb of chronically treated animals, 2) those from the operated side of animals that had been deafferented and hemispinalized but not subjected to chronic stimulation, 3) those from normal animals that had not been subjected to chronic treatment. With respect to the presently studied parameters, the three kinds of control muscles rendered very similar results. 4. All the presently used patterns of chronic stimulation made the PerL muscles slower with respect to twitch contraction time, half-relaxation time, and tension-frequency relation. Patterns covering 5-5.5% of daily time (F1, S1, S1F2) also caused an increase in the percentage of fibers classified as 'slow' (type I) on basis of their staining for myosin adenosine triphosphatase (ATPase). 5. Among patterns covering 5% of daily time, the change in ATPase histochemistry and the degree of physiological slowing was at least as pronounced after chronic stimulation at 100 Hz (F1) as after treatment at 10 Hz (S1). The slowing produced by pattern S1 was not more pronounced than that caused by this pattern (10 Hz) plus an equal number of pulses at 100 Hz (S1F2). 6. The slowing produced by the presently used patterns of chronic stimulation took place within the initial 2-3 wk. 7. Patterns F1 and S1 caused a decrease in maximum tetanic force as well as in mean fiber diameter.(ABSTRACT TRUNCATED AT 400 WORDS)

This article has been cited by other articles:

				T. Eken, G. C. B. Elder, and T. Lomo Development of Tonic Firing Behavior in Rat Soleus Muscle J Neurophysiol, April 1, 2008; 99(4): 1899 - 1905. [Abstract] [Full Text] [PDF]

				C. K. Hager-Ross, C. S. Klein, and C. K. Thomas Twitch and Tetanic Properties of Human Thenar Motor Units Paralyzed by Chronic Spinal Cord Injury J Neurophysiol, July 1, 2006; 96(1): 165 - 174. [Abstract] [Full Text] [PDF]

				C. S. Klein, C. K. Hager-Ross, and C. K. Thomas Fatigue properties of human thenar motor units paralysed by chronic spinal cord injury J. Physiol., May 15, 2006; 573(1): 161 - 171. [Abstract] [Full Text] [PDF]

				R. L. W. Harris, J. Bobet, L. Sanelli, and D. J. Bennett Tail Muscles Become Slow but Fatigable in Chronic Sacral Spinal Rats With Spasticity J Neurophysiol, February 1, 2006; 95(2): 1124 - 1133. [Abstract] [Full Text] [PDF]

				B. Reid, V. N. Martinov, A. Nja, T. Lomo, and G. S. Bewick Activity-Dependent Plasticity of Transmitter Release from Nerve Terminals in Rat Fast and Slow Muscles J. Neurosci., October 15, 2003; 23(28): 9340 - 9348. [Abstract] [Full Text] [PDF]

				A. Lopez-Guajardo, H. Sutherland, J. C. Jarvis, and S. Salmons Induction of a fatigue-resistant phenotype in rabbit fast muscle by small daily amounts of stimulation J Appl Physiol, May 1, 2001; 90(5): 1909 - 1918. [Abstract] [Full Text] [PDF]

				T. Gordon, N. Tyreman, V. F. Rafuse, and J. B. Munson Fast-to-Slow Conversion Following Chronic Low-Frequency Activation of Medial Gastrocnemius Muscle in Cats. I. Muscle and Motor Unit Properties J Neurophysiol, May 1, 1997; 77(5): 2585 - 2604. [Abstract] [Full Text] [PDF]

				R. K. Shields, L. F. Law, B. Reiling, K. Sass, and J. Wilwert Effects of electrically induced fatigue on the twitch and tetanus of paralyzed soleus muscle in humans J Appl Physiol, May 1, 1997; 82(5): 1499 - 1507. [Abstract] [Full Text] [PDF]