JN Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 68: 197-212, 1992;
0022-3077/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Staley, K. J.
Right arrow Articles by Mody, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Staley, K. J.
Right arrow Articles by Mody, I.

Journal of Neurophysiology, Vol 68, Issue 1 197-212, Copyright © 1992 by APS

ARTICLES

Shunting of excitatory input to dentate gyrus granule cells by a depolarizing GABAA receptor-mediated postsynaptic conductance

K. J. Staley and I. Mody
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, California 94305.

1. Stimulation of the perforant path in the outer molecular layer of the adult rat dentate gyrus produced a depolarizing post-synaptic potential (DPSP) in granule cells when recorded using whole-cell techniques in the standard hippocampal slice preparation at 34 degrees C. The postsynaptic currents (PSCs) contributing to the DPSP were analyzed using specific receptor antagonists in current- and voltage-clamp recordings. 2. The DPSP reversal potential was dependent on the intracellular chloride concentration, and the amplitude of the DPSP was increased 55% after perfusion of the gamma-aminobutyric acid-A (GABAA) receptor antagonist bicuculline methiodide (BMI). The GABAA receptor-mediated PSC reversed at -66 mV, which was 19 mV positive to the resting membrane potential (-85 mV) but hyperpolarized relative to action potential threshold. At -35 mV, the GABAA PSC had a latency to peak of 12.9 ms after the stimulus and decayed monoexponentially with an average time constant of 23.4 ms. 3. The component of the PSC blocked by the Quis/AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) had a latency to peak of 7.1 ms and decayed monoexponentially with a time constant of 9.9 ms at -35 mV. The N-methyl-D-aspartate (NMDA) receptor-mediated PSC, which was blocked by D-amino-5-phosphonovaleric acid (D-AP5), had a waveform that was similar to the GABAA PSC: the latency to peak was 16 ms and the decay was monoexponential with a time constant of 24.5 ms at -35 mV. 4. The ratio of the peak PSCs mediated by GABAA, Quis/AMPA, and NMDA receptors measured at -35 mV with cesium gluconate electrode solutions was 1:0.2:0.1. This ratio was essentially constant over the range of stimulus intensities that produced compound PSC amplitudes of 80-400 pA. 5. Measured at its reversal potential, the GABAA receptor-mediated postsynaptic conductance (GGABA-A) decreased the peak DPSP amplitude by 35%, shunted 50% of the charge transferred to the soma by the excitatory PSC, and completely inhibited the NMDA receptor-mediated component of the DPSP. 6. Simultaneous stimulation of presynaptic fibers from both the perforant path and interneurons results in a large depolarizing GGABA-A that inhibits the granule cell by shunting the excitatory PSCs. As predicted by models of shunting, the similar kinetics of the GABAA and NMDA PSCs leads to particularly effective inhibition of the NMDA PSC. The more rapid Quis/AMPA PSC is less affected by the GGABA-A, so that granule cell excitation under these conditions is primarily due to Quis/AMPA receptor activation.


This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
R. Riekki, I. Pavlov, J. Tornberg, S. E. Lauri, M. S. Airaksinen, and T. Taira
Altered Synaptic Dynamics and Hippocampal Excitability but Normal Long-Term Plasticity in Mice Lacking Hyperpolarizing GABAA Receptor-Mediated Inhibition in CA1 Pyramidal Neurons
J Neurophysiol, June 1, 2008; 99(6): 3075 - 3089.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. C. Brumback and K. J. Staley
Thermodynamic Regulation of NKCC1-Mediated Cl- Cotransport Underlies Plasticity of GABAA Signaling in Neonatal Neurons
J. Neurosci., February 6, 2008; 28(6): 1301 - 1312.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
Y. Ben-Ari, J.-L. Gaiarsa, R. Tyzio, and R. Khazipov
GABA: A Pioneer Transmitter That Excites Immature Neurons and Generates Primitive Oscillations
Physiol Rev, October 1, 2007; 87(4): 1215 - 1284.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Achilles, A. Okabe, M. Ikeda, C. Shimizu-Okabe, J. Yamada, A. Fukuda, H. J. Luhmann, and W. Kilb
Kinetic Properties of Cl Uptake Mediated by Na+-Dependent K+-2Cl Cotransport in Immature Rat Neocortical Neurons
J. Neurosci., August 8, 2007; 27(32): 8616 - 8627.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. M. Schofield and J. R. Huguenard
GABA Affinity Shapes IPSCs in Thalamic Nuclei
J. Neurosci., July 25, 2007; 27(30): 7954 - 7962.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. T. Paz, M. Chavez, S. Saillet, J.-M. Deniau, and S. Charpier
Activity of Ventral Medial Thalamic Neurons during Absence Seizures and Modulation of Cortical Paroxysms by the Nigrothalamic Pathway
J. Neurosci., January 24, 2007; 27(4): 929 - 941.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. S. Hekmat-Scafe, M. Y. Lundy, R. Ranga, and M. A. Tanouye
Mutations in the K+/Cl- Cotransporter Gene kazachoc (kcc) Increase Seizure Susceptibility in Drosophila.
J. Neurosci., August 30, 2006; 26(35): 8943 - 8954.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
W. Song, S. C. Chattipakorn, and L. L. McMahon
Glycine-Gated Chloride Channels Depress Synaptic Transmission in Rat Hippocampus
J Neurophysiol, April 1, 2006; 95(4): 2366 - 2379.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. S. Overstreet-Wadiche, A. L. Bensen, and G. L. Westbrook
Delayed development of adult-generated granule cells in dentate gyrus.
J. Neurosci., February 22, 2006; 26(8): 2326 - 2334.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. M. Moenter and R. A. DeFazio
Endogenous {gamma}-Aminobutyric Acid Can Excite Gonadotropin-Releasing Hormone Neurons
Endocrinology, December 1, 2005; 146(12): 5374 - 5379.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Ge and J. A. Dani
Nicotinic Acetylcholine Receptors at Glutamate Synapses Facilitate Long-Term Depression or Potentiation
J. Neurosci., June 29, 2005; 25(26): 6084 - 6091.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
F. Wang, C. Xiao, and J. H. Ye
Taurine activates excitatory non-synaptic glycine receptors on dopamine neurones in ventral tegmental area of young rats
J. Physiol., June 1, 2005; 565(2): 503 - 516.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. E. Talpalar and Y. Grossman
Enhanced Excitability Compensates for High-Pressure-Induced Depression of Cortical Inputs to the Hippocampus
J Neurophysiol, December 1, 2004; 92(6): 3309 - 3319.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
F. Laezza and R. Dingledine
Voltage-Controlled Plasticity at GluR2-Deficient Synapses Onto Hippocampal Interneurons
J Neurophysiol, December 1, 2004; 92(6): 3575 - 3581.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. M. Petrini, I. Marchionni, P. Zacchi, W. Sieghart, and E. Cherubini
Clustering of Extrasynaptic GABAA Receptors Modulates Tonic Inhibition in Cultured Hippocampal Neurons
J. Biol. Chem., October 29, 2004; 279(44): 45833 - 45843.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. J. Slaght, T. Paz, M. Chavez, J.-M. Deniau, S. Mahon, and S. Charpier
On the Activity of the Corticostriatal Networks during Spike-and-Wave Discharges in a Genetic Model of Absence Epilepsy
J. Neurosci., July 28, 2004; 24(30): 6816 - 6825.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. Galeffi, R. Sah, B. B. Pond, A. George, and R. D. Schwartz-Bloom
Changes in Intracellular Chloride after Oxygen-Glucose Deprivation of the Adult Hippocampal Slice: Effect of Diazepam
J. Neurosci., May 5, 2004; 24(18): 4478 - 4488.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Z. Jiang, K. Krnjevic, F. Wang, and J. H. Ye
Taurine Activates Strychnine-Sensitive Glycine Receptors in Neurons Freshly Isolated From Nucleus Accumbens of Young Rats
J Neurophysiol, January 1, 2004; 91(1): 248 - 257.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
W. Wei, N. Zhang, Z. Peng, C. R. Houser, and I. Mody
Perisynaptic Localization of {delta} Subunit-Containing GABAA Receptors and Their Activation by GABA Spillover in the Mouse Dentate Gyrus
J. Neurosci., November 19, 2003; 23(33): 10650 - 10661.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. E. Talpalar and Y. Grossman
Modulation of Rat Corticohippocampal Synaptic Activity by High Pressure and Extracellular Calcium: Single and Frequency Responses
J Neurophysiol, October 1, 2003; 90(4): 2106 - 2114.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. J. Wierenga and W. J. Wadman
Excitatory Inputs to CA1 Interneurons Show Selective Synaptic Dynamics
J Neurophysiol, August 1, 2003; 90(2): 811 - 821.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Chavas and A. Marty
Coexistence of Excitatory and Inhibitory GABA Synapses in the Cerebellar Interneuron Network
J. Neurosci., March 15, 2003; 23(6): 2019 - 2031.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. I. Dzhala and K. J. Staley
Excitatory Actions of Endogenously Released GABA Contribute to Initiation of Ictal Epileptiform Activity in the Developing Hippocampus
J. Neurosci., March 1, 2003; 23(5): 1840 - 1846.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S.-K. Han, I. M. Abraham, and A. E. Herbison
Effect of GABA on GnRH Neurons Switches from Depolarization to Hyperpolarization at Puberty in the Female Mouse
Endocrinology, April 1, 2002; 143(4): 1459 - 1466.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. V. Buhler and T. V. Dunwiddie
alpha 7 Nicotinic Acetylcholine Receptors on GABAergic Interneurons Evoke Dendritic and Somatic Inhibition of Hippocampal Neurons
J Neurophysiol, January 1, 2002; 87(1): 548 - 557.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. J. Staley, J. S. Bains, A. Yee, J. Hellier, and J. M. Longacher
Statistical Model Relating CA3 Burst Probability to Recovery From Burst-Induced Depression at Recurrent Collateral Synapses
J Neurophysiol, December 1, 2001; 86(6): 2736 - 2747.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. A. O'Brien and A. J. Berger
The Nonuniform Distribution of the GABAA Receptor alpha 1 Subunit Influences Inhibitory Synaptic Transmission to Motoneurons within a Motor Nucleus
J. Neurosci., November 1, 2001; 21(21): 8482 - 8494.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Monsivais and E. W Rubel
Accommodation Enhances Depolarizing Inhibition in Central Neurons
J. Neurosci., October 1, 2001; 21(19): 7823 - 7830.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y.-F. Wang, X.-B. Gao, and A. N. van den Pol
Membrane Properties Underlying Patterns of GABA-Dependent Action Potentials in Developing Mouse Hypothalamic Neurons
J Neurophysiol, September 1, 2001; 86(3): 1252 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Baufreton, M. Garret, S. Dovero, B. Dufy, B. Bioulac, and A. Taupignon
Activation of GABAA Receptors in Subthalamic Neurons In Vitro: Properties of Native Receptors and Inhibition Mechanisms
J Neurophysiol, July 1, 2001; 86(1): 75 - 85.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. S. Fitzpatrick, G. Akopian, and J. P. Walsh
Short-Term Plasticity at Inhibitory Synapses in Rat Striatum and Its Effects on Striatal Output
J Neurophysiol, May 1, 2001; 85(5): 2088 - 2099.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. E. Wells, J. T. Porter, and A. Agmon
GABAergic Inhibition Suppresses Paroxysmal Network Activity in the Neonatal Rodent Hippocampus and Neocortex
J. Neurosci., December 1, 2000; 20(23): 8822 - 8830.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. P. Wong, G. Marchese, M. A. Casu, A. Ribeiro-da-Silva, A. C. Cuello, and Y. De Koninck
Loss of Presynaptic and Postsynaptic Structures Is Accompanied by Compensatory Increase in Action Potential-Dependent Synaptic Input to Layer V Neocortical Pyramidal Neurons in Aged Rats
J. Neurosci., November 15, 2000; 20(22): 8596 - 8606.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M.L.H.J. Hermes, J. M. Ruijter, A. Klop, R. M. Buijs, and L. P. Renaud
Vasopressin Increases GABAergic Inhibition of Rat Hypothalamic Paraventricular Nucleus Neurons In Vitro
J Neurophysiol, February 1, 2000; 83(2): 705 - 711.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. M. Palva, K. Lamsa, S. E. Lauri, H. Rauvala, K. Kaila, and T. Taira
Fast Network Oscillations in the Newborn Rat Hippocampus In Vitro
J. Neurosci., February 1, 2000; 20(3): 1170 - 1178.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Lamsa, J. M. Palva, E. Ruusuvuori, K. Kaila, and T. Taira
Synaptic GABAA Activation Inhibits AMPA-Kainate Receptor-Mediated Bursting in the Newborn (P0-P2) Rat Hippocampus
J Neurophysiol, January 1, 2000; 83(1): 359 - 366.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
D. D. Mott, Q. Li, M. M. Okazaki, D. A. Turner, and D. V. Lewis
GABAB-Receptor-Mediated Currents in Interneurons of the Dentate-Hilus Border
J Neurophysiol, September 1, 1999; 82(3): 1438 - 1450.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. M. Okazaki, P. Molnar, and J. V. Nadler
Recurrent Mossy Fiber Pathway in Rat Dentate Gyrus: Synaptic Currents Evoked in Presence and Absence of Seizure-Induced Growth
J Neurophysiol, April 1, 1999; 81(4): 1645 - 1660.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. A. H. Verheugen, D. Fricker, and R. Miles
Noninvasive Measurements of the Membrane Potential and GABAergic Action in Hippocampal Interneurons
J. Neurosci., April 1, 1999; 19(7): 2546 - 2555.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. F. Jackson, B. Esplin, and R. Capek
Inhibitory Nature of Tiagabine-Augmented GABAA Receptor-Mediated Depolarizing Responses in Hippocampal Pyramidal Cells
J Neurophysiol, March 1, 1999; 81(3): 1192 - 1198.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y. B. Liu, G.-L. Ye, X.-S. Liu, J. F. Pasternak, and B. L. Trommer
GABAA Currents in Immature Dentate Gyrus Granule Cells
J Neurophysiol, November 1, 1998; 80(5): 2255 - 2267.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. S. Hollrigel, S. T. Ross, and I. Soltesz
Temporal Patterns and Depolarizing Actions of Spontaneous GABAA Receptor Activation in Granule Cells of the Early Postnatal Dentate Gyrus
J Neurophysiol, November 1, 1998; 80(5): 2340 - 2351.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Berzhanskaya, N. N. Urban, and G. Barrionuevo
Electrophysiological and Pharmacological Characterization of the Direct Perforant Path Input to Hippocampal Area CA3
J Neurophysiol, April 1, 1998; 79(4): 2111 - 2118.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Z. Nusser, W. Sieghart, and P. Somogyi
Segregation of Different GABAA Receptors to Synaptic and Extrasynaptic Membranes of Cerebellar Granule Cells
J. Neurosci., March 1, 1998; 18(5): 1693 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Lamsa and K. Kaila
Ionic Mechanisms of Spontaneous GABAergic Events in Rat Hippocampal Slices Exposed to 4-Aminopyridine
J Neurophysiol, November 1, 1997; 78(5): 2582 - 2591.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Kaneko and D. L. Hammond
Role of Spinal gamma -Aminobutyric AcidA Receptors in Formalin-Induced Nociception in the Rat
J. Pharmacol. Exp. Ther., August 1, 1997; 282(2): 928 - 938.
[Abstract] [Full Text]

Home page
 J. Neurophysiol.Home page
W. R. Holmes and W. B. Levy
Quantifying the Role of Inhibition in Associative Long-Term Potentiation in Dentate Granule Cells With Computational Models
J Neurophysiol, July 1, 1997; 78(1): 103 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. S. Hollrigel and I. Soltesz
Slow Kinetics of Miniature IPSCs during Early Postnatal Development in Granule Cells of the Dentate Gyrus
J. Neurosci., July 1, 1997; 17(13): 5119 - 5128.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. D. Mott, D. A. Turner, M. M. Okazaki, and D. V. Lewis
Interneurons of the Dentate-Hilus Border of the Rat Dentate Gyrus: Morphological and Electrophysiological Heterogeneity
J. Neurosci., June 1, 1997; 17(11): 3990 - 4005.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
K. Staley, B. Soldo, and W. Proctor
Ionic mechanisms of neuronal excitation by inhibitory GABAA receptors
Science, August 18, 1995; 269(5226): 977 - 981.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. T. Ross and I. Soltesz
Long-term plasticity in interneurons of the dentate gyrus
PNAS, July 17, 2001; 98(15): 8874 - 8879.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online