JN Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 70: 167-178, 1993;
0022-3077/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ye, Q.
Right arrow Articles by DeSimone, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ye, Q.
Right arrow Articles by DeSimone, J. A.

Journal of Neurophysiology, Vol 70, Issue 1 167-178, Copyright © 1993 by APS

ARTICLES

Voltage dependence of the rat chorda tympani response to Na+ salts: implications for the functional organization of taste receptor cells

Q. Ye, G. L. Heck and J. A. DeSimone
Department of Physiology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0551.

1. Voltage-clamp and current-clamp data were obtained from a circumscribed region of the anterior rat lingual epithelium while simultaneously monitoring the afferent, stimulus-evoked, neural response from the same receptive field. 2. Chorda tympani (CT) responses at constant Na(+)-salt concentration were enhanced by submucosa negative voltage clamp and suppressed by positive voltage clamp. The complete CT response profile, including the time course of adaptation, was not uniquely determined by NaCl concentration alone. The response could be reproduced at different NaCl concentrations by applying a compensating voltage. 3. The form of the concentration and voltage dependence of the CT response indicates that the complete stimulus energy is the Na+ electrochemical potential difference across receptor cell apical membranes, and not Na+ concentration alone. This is the underlying principal behind the equivalence of chemical and electric taste for Na+ salts. 4. CT responses to sodium gluconate (25 and 200 mM) and 25 mM NaCl produced amiloride-insensitive components (AIC) of low magnitude. NaCl at 200 mM produced a significantly larger AIC. The AIC was voltage-clamp independent. The relative magnitude of the AIC was positively correlated with the transepithelial conductance of each salt. This suggests that the large AIC for 200 mM NaCl results from its relatively high permeability through the paracellular pathway. 5. Analysis of the CT response under voltage clamp revealed two anion effects on Na(+)-salt taste, both of which act through the paracellular shunt. 1) Anions modify the transepithelial potential (TP) across tight junctions and thereby modulate the cell receptor potential. This anion effect can be eliminated by voltage clamping the TP. 2) Sufficiently mobile anions facilitate electroneutral diffusion of Na+ salts through tight junctions. This effect is observed especially when Cl- is the anion and when the stimulus concentration favors NaCl influx, allowing Na+ to stimulate receptor cells from the submucosal side. Because the submucosal intercellular spaces are nearly isopotential regions, this effect is insensitive to voltage clamp of the TP. The large AIC associated with this anion effect is due to the low permeability of amiloride.


This article has been cited by other articles:


Home page
 Chem SensesHome page
J. M. Breza, K. S. Curtis, and R. J. Contreras
Monosodium Glutamate but not Linoleic Acid Differentially Activates Gustatory Neurons in the Rat Geniculate Ganglion
Chem Senses, November 1, 2007; 32(9): 833 - 846.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
S. D. Hillyard, V. Baula, W. Tuttle, N. J. Willumsen, and E. H. Larsen
Behavioral and Neural Responses of Toads to Salt Solutions Correlate with Basolateral Membrane Potential of Epidermal Cells of the Skin
Chem Senses, October 1, 2007; 32(8): 765 - 773.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Treesukosol, V. Lyall, G. L. Heck, J. A. DeSimone, and A. C. Spector
A psychophysical and electrophysiological analysis of salt taste in Trpv1 null mice
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1799 - R1809.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
C. Ruiz, S. Gutknecht, E. Delay, and S. Kinnamon
Detection of NaCl and KCl in TRPV1 Knockout Mice
Chem Senses, November 1, 2006; 31(9): 813 - 820.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
T. Ohkuri, K. Yasumatsu, N. Shigemura, R. Yoshida, and Y. Ninomiya
Amiloride Inhibition on NaCl Responses of the Chorda Tympani Nerve in Two 129 Substrains of Mice, 129P3/J and 129X1/SvJ
Chem Senses, July 1, 2006; 31(6): 565 - 572.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
J. Lim and H. T. Lawless
Qualitative Differences of Divalent Salts: Multidimensional Scaling and Cluster Analysis
Chem Senses, November 1, 2005; 30(9): 719 - 726.
[Abstract] [Full Text] [PDF]

Home page
 Behav Cogn Neurosci RevHome page
A. C. Spector and S. P. Travers
The representation of taste quality in the Mammalian nervous system.
Behav Cogn Neurosci Rev, September 1, 2005; 4(3): 143 - 191.
[Abstract] [PDF]

Home page
 Chem SensesHome page
N. Shigemura, A. A. S. Islam, C. Sadamitsu, R. Yoshida, K. Yasumatsu, and Y. Ninomiya
Expression of Amiloride-sensitive Epithelial Sodium Channels in Mouse Taste Cells after Chorda Tympani Nerve Crush
Chem Senses, July 1, 2005; 30(6): 531 - 538.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
V. Lyall, G. L. Heck, T.-H. T. Phan, S. Mummalaneni, S. A. Malik, A. K. Vinnikova, and J. A. DeSimone
Ethanol Modulates the VR-1 Variant Amiloride-insensitive Salt Taste Receptor. I. Effect on TRC Volume and Na+ Flux
J. Gen. Physiol., May 31, 2005; 125(6): 569 - 585.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
V. Lyall, G. L. Heck, T.-H. T. Phan, S. Mummalaneni, S. A. Malik, A. K. Vinnikova, and J. A. DeSimone
Ethanol Modulates the VR-1 Variant Amiloride-insensitive Salt Taste Receptor. II. Effect on Chorda Tympani Salt Responses
J. Gen. Physiol., May 31, 2005; 125(6): 587 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Eylam and A. C. Spector
Taste discrimination between NaCl and KCl is disrupted by amiloride in inbred mice with amiloride-insensitive chorda tympani nerves
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1361 - R1368.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. V. Verhagen, B. K. Giza, and T. R. Scott
Effect of Amiloride on Gustatory Responses in the Ventroposteromedial Nucleus of the Thalamus in Rats
J Neurophysiol, January 1, 2005; 93(1): 157 - 166.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. Lyall, G. L. Heck, A. K. Vinnikova, S. Ghosh, T.-H. T. Phan, R. I. Alam, O. F. Russell, S. A. Malik, J. W. Bigbee, and J. A. DeSimone
The mammalian amiloride-insensitive non-specific salt taste receptor is a vanilloid receptor-1 variant
J. Physiol., July 1, 2004; 558(1): 147 - 159.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. Lyall, R. I. Alam, S. A. Malik, T.-H. T. Phan, A. K. Vinnikova, G. L. Heck, and J. A. DeSimone
Basolateral Na+-H+ exchanger-1 in rat taste receptor cells is involved in neural adaptation to acidic stimuli
J. Physiol., April 1, 2004; 556(1): 159 - 173.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
S. Eylam, T. Tracy, M. Garcea, and A. C. Spector
Amiloride is an Ineffective Conditioned Stimulus in Taste Aversion Learning in C57BL/6J and DBA/2J Mice
Chem Senses, October 1, 2003; 28(8): 681 - 689.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. M. Feldman, A. Mogyorosi, G. L. Heck, J. A. DeSimone, C. R. Santos, R. A. Clary, and V. Lyall
Salt-Evoked Lingual Surface Potential in Humans
J Neurophysiol, September 1, 2003; 90(3): 2060 - 2064.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
S. Eylam and A. C. Spector
Oral Amiloride Treatment Decreases Taste Sensitivity to Sodium Salts in C57BL/6J and DBA/2J Mice
Chem Senses, June 1, 2003; 28(5): 447 - 458.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Yasumatsu, H. Katsukawa, K. Sasamoto, and Y. Ninomiya
Recovery of Amiloride-Sensitive Neural Coding during Regeneration of the Gustatory Nerve: Behavioral-Neural Correlation of Salt Taste Discrimination
J. Neurosci., May 15, 2003; 23(10): 4362 - 4368.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
S. A. Simon
Interactions between Salt and Acid Stimuli: A Lesson in Gustation from Simultaneous Epithelial and Neural Recordings
J. Gen. Physiol., November 25, 2002; 120(6): 787 - 791.
[Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
V. Lyall, R. I. Alam, T.-H. T. Phan, O. F. Russell, S. A. Malik, G. L. Heck, and J. A. DeSimone
Modulation of Rat Chorda Tympani NaCl Responses and Intracellular Na+ Activity in Polarized Taste Receptor Cells by pH
J. Gen. Physiol., November 25, 2002; 120(6): 793 - 815.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. C. Geran, M. Garcea, and A. C. Spector
Transecting the gustatory branches of the facial nerve impairs NH4Cl vs. KCl discrimination in rats
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R739 - R747.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
V. Lyall, R. I. Alam, T.-H. T. Phan, D. Q. Phan, G. L. Heck, and J. A. DeSimone
Excitation and Adaptation in the Detection of Hydrogen Ions by Taste Receptor Cells: A Role for cAMP and Ca2+
J Neurophysiol, January 1, 2002; 87(1): 399 - 408.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
Y. Kitada, K. Okuda-Akabane, and Y. Mitoh
Effects of Amiloride on Gustatory Neural Responses to Salts in the Frog
Chem Senses, November 1, 2001; 26(9): 1203 - 1210.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
T. Nagai, D. Nii, and H.-a. Takeuchi
Amiloride Blocks Salt Taste Transduction of the Glossopharyngeal Nerve in Metamorphosed Salamanders
Chem Senses, October 1, 2001; 26(8): 965 - 969.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
V. Lyall, R. I. Alam, D. Q. Phan, G. L. Ereso, T.-H. T. Phan, S. A. Malik, M. H. Montrose, S. Chu, G. L. Heck, G. M. Feldman, et al.
Decrease in rat taste receptor cell intracellular pH is the proximate stimulus in sour taste transduction
Am J Physiol Cell Physiol, September 1, 2001; 281(3): C1005 - C1013.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
T. Miyamoto, T. Miyazaki, R. Fujiyama, Y. Okada, and T. Sato
Differential Transduction Mechanisms Underlying NaCl- and KCl-induced Responses in Mouse Taste Cells
Chem Senses, January 1, 2001; 26(1): 67 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
S. I. Sollars and D. L. Hill
Lack of Functional and Morphological Susceptibility of the Greater Superficial Petrosal Nerve to Developmental Dietary Sodium Restriction
Chem Senses, December 1, 2000; 25(6): 719 - 727.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. J. Hendricks, R. E. Stewart, G. L. Heck, J. A. DeSimone, and D. L. Hill
Development of Rat Chorda Tympani Sodium Responses: Evidence for Age-Dependent Changes in Global Amiloride-Sensitive Na+ Channel Kinetics
J Neurophysiol, September 1, 2000; 84(3): 1531 - 1544.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. J. St. John and D. V. Smith
Neural Representation of Salts in the Rat Solitary Nucleus: Brain Stem Correlates of Taste Discrimination
J Neurophysiol, August 1, 2000; 84(2): 628 - 638.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
P. A. Sullivan, K. von Seckendorff Hoff, and S. D. Hillyard
Effects of Anion Substitution on Hydration Behavior and Water Uptake of the Red-spotted Toad, Bufo punctatus: is there an Anion Paradox in Amphibian Skin?
Chem Senses, April 1, 2000; 25(2): 167 - 172.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. L. Kopka, L. C. Geran, and A. C. Spector
Functional status of the regenerated chorda tympani nerve as assessed in a salt taste discrimination task
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2000; 278(3): R720 - R731.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
V. Lyall, G. L. Heck, J. A. DeSimone, and G. M. Feldman
Effects of osmolarity on taste receptor cell size and function
Am J Physiol Cell Physiol, October 1, 1999; 277(4): C800 - C813.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. D. Boughter Jr., S. J. St. John, and D. V. Smith
Neural Representation of the Taste of NaCl and KCl in Gustatory Neurons of the Hamster Solitary Nucleus
J Neurophysiol, June 1, 1999; 81(6): 2636 - 2646.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
O. Kretz, P. Barbry, R. Bock, and B. Lindemann
Differential Expression of RNA and Protein of the Three Pore-forming Subunits of the Amiloride-sensitive Epithelial Sodium Channel in Taste Buds of the Rat
J. Histochem. Cytochem., January 1, 1999; 47(1): 51 - 64.
[Abstract] [Full Text]

Home page
 J. Neurophysiol.Home page
J. D. Boughter Jr. and D. V. Smith
Amiloride Blocks Acid Responses in NaCl-Best Gustatory Neurons of the Hamster Solitary Nucleus
J Neurophysiol, September 1, 1998; 80(3): 1362 - 1372.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. E. Stewart, V. Lyall, G. M. Feldman, G. L. Heck, and J. A. DeSimone
Acid-induced responses in hamster chorda tympani and intracellular pH tracking by taste receptor cells
Am J Physiol Cell Physiol, July 1, 1998; 275(1): C227 - C238.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. A. Kloub, G. L. Heck, and J. A. Desimone
Self-Inhibition in Ca2+-Evoked Taste Responses: A Novel Tool for Functional Dissection of Salt Taste Transduction Mechanisms
J Neurophysiol, February 1, 1998; 79(2): 911 - 921.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. F. Lundy Jr., D. W. Pittman, and R. J. Contreras
Role for epithelial Na+ channels and putative Na+/H+ exchangers in salt taste transduction in rats
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 1997; 273(6): R1923 - R1931.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H. Nishijo and R. Norgren
Parabrachial Neural Coding of Taste Stimuli in Awake Rats
J Neurophysiol, November 1, 1997; 78(5): 2254 - 2268.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. A. Kloub, G. L. Heck, and J. A. Desimone
Chorda Tympani Responses Under Lingual Voltage Clamp: Implications for NH4 Salt Taste Transduction
J Neurophysiol, March 1, 1997; 77(3): 1393 - 1406.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online