| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Neurophysiology, Vol 70, Issue 4 1420-1432, Copyright © 1993 by APS
ARTICLES |
M. I. Banks, R. A. Pearce and P. H. Smith
Neuroscience Training Program, University of Wisconsin, Madison 53706.
1. Principal cells in the medial nucleus of the trapezoid body (MNTB) are part of a circuit in the superior olivary complex (SOC) that processes binaural information important for sound localization. MNTB cells have two voltage-dependent currents active near rest that contribute to these cells' highly nonlinear membrane properties and shape their responses to synaptic input. One of these currents, a low-threshold, 4-aminopyridine (4-AP)-sensitive K+ current, has been studied previously under current clamp. Using the single-electrode voltage-clamp technique, we have investigated the other of these currents, a hyperpolarization-activated, mixed cation current (Ih), in brain slices of the rat SOC. 2. Ih is responsible for a prominent "sag" in the voltage response to a steady hyperpolarizing current recorded under current clamp in MNTB cells. In voltage-clamp recordings, hyperpolarizing voltage steps from the resting potential elicited a large inward current that activated and deactivated with biexponential kinetics. Activation time constants were voltage dependent, with tau 1 and tau 2 = 246 and 1620 ms at -75 mV and 107 and 560 ms at -100 mV. 3. Ih was blocked by 1-5 mM cesium and had a reversal potential of -43 mV. Steady-state activation curves derived from tail currents yielded a half-activation voltage of -75.7 mV and slope factor of 5.7 mV, corresponding to < 10% activation of Ih at rest. 4. Application of norepinephrine (15-20 microM) or 8-bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) (1 mM) caused a depolarizing shift in the steady-state activation curve and decreased the activation time constants. The shift in the activation curve resulted in a large increase in the activation of Ih at rest, an inward shift in the holding current, and an increase in the resting membrane conductance. In current-clamp recordings, this increase in the resting activation level of Ih resulted in membrane depolarization of 2-3 mV in the absence of 4-AP, and 5-10 mV in the presence of 4-AP, an increase in the input conductance, and a reduction in the voltage sag in response to hyperpolarizing currents. 5. The resulting change in the resting point of MNTB cells exposed to norepinephrine or 8-Br-cAMP is likely to alter the responses of these cells to synaptic input, both via the direct effect on the resting membrane conductance and by changing the activation of the low-threshold, 4-AP-sensitive potassium current.(ABSTRACT TRUNCATED AT 400 WORDS)
This article has been cited by other articles:
|
|
 |
|
  M. L. Tan and J.G.G. Borst Comparison of Responses of Neurons in the Mouse Inferior Colliculus to Current Injections, Tones of Different Durations, and Sinusoidal Amplitude-Modulated Tones J Neurophysiol, July 1, 2007; 98(1): 454 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-J. Cao, S. Shatadal, and D. Oertel Voltage-Sensitive Conductances of Bushy Cells of the Mammalian Ventral Cochlear Nucleus J Neurophysiol, June 1, 2007; 97(6): 3961 - 3975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. I. Verbny, F. Erdelyi, G. Szabo, and M. I. Banks Properties of a Population of GABAergic Cells in Murine Auditory Cortex Weakly Excited by Thalamic Stimulation J Neurophysiol, December 1, 2006; 96(6): 3194 - 3208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Leao, R. N. Leao, H. Sun, R. E. W. Fyffe, and B. Walmsley Hyperpolarization-activated currents are differentially expressed in mice brainstem auditory nuclei J. Physiol., November 1, 2006; 576(3): 849 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. X. Gittelman and B. L Tempel Kv1.1-Containing Channels Are Critical for Temporal Precision During Spike Initiation J Neurophysiol, September 1, 2006; 96(3): 1203 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. A. Rodrigues and D. Oertel Hyperpolarization-Activated Currents Regulate Excitability in Stellate Cells of the Mammalian Ventral Cochlear Nucleus J Neurophysiol, January 1, 2006; 95(1): 76 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Yamada, H. Kuba, T. M. Ishii, and H. Ohmori Hyperpolarization-Activated Cyclic Nucleotide-Gated Cation Channels Regulate Auditory Coincidence Detection in Nucleus Laminaris of the Chick J. Neurosci., September 28, 2005; 25(39): 8867 - 8877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lu and E. W Rubel Activation of Metabotropic Glutamate Receptors Inhibits High-Voltage-Gated Calcium Channel Currents of Chicken Nucleus Magnocellularis Neurons J Neurophysiol, March 1, 2005; 93(3): 1418 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kang, T. Notomi, M. Saito, W. Zhang, and R. Shigemoto Bidirectional Interactions between H-Channels and Na+-K+ Pumps in Mesencephalic Trigeminal Neurons J. Neurosci., April 7, 2004; 24(14): 3694 - 3702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Koch and B. Grothe Hyperpolarization-Activated Current (Ih) in the Inferior Colliculus: Distribution and Contribution to Temporal Processing J Neurophysiol, December 1, 2003; 90(6): 3679 - 3687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Rothman and P. B. Manis The Roles Potassium Currents Play in Regulating the Electrical Activity of Ventral Cochlear Nucleus Neurons J Neurophysiol, June 1, 2003; 89(6): 3097 - 3113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tanaka, N. Wu, C.-F. Hsaio, J. Turman Jr., and S. H. Chandler Development of Inward Rectification and Control of Membrane Excitability in Mesencephalic V Neurons J Neurophysiol, March 1, 2003; 89(3): 1288 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Leao and H. Von Gersdorff Noradrenaline Increases High-Frequency Firing at the Calyx of Held Synapse During Development by Inhibiting Glutamate Release J Neurophysiol, May 1, 2002; 87(5): 2297 - 2306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Chapin and R. Andrade A 5-HT7 Receptor-Mediated Depolarization in the Anterodorsal Thalamus. II. Involvement of the Hyperpolarization-Activated Current Ih J. Pharmacol. Exp. Ther., April 1, 2001; 297(1): 403 - 409. [Abstract] [Full Text]
|
|
|
|
|
|
F. Saitow, S.'I. Satake, J. Yamada, and S. Konishi beta -Adrenergic Receptor-Mediated Presynaptic Facilitation of Inhibitory GABAergic Transmission at Cerebellar Interneuron-Purkinje Cell Synapses J Neurophysiol, October 1, 2000; 84(4): 2016 - 2025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Saitow and S. Konishi Excitability Increase Induced by beta -Adrenergic Receptor-Mediated Activation of Hyperpolarization-Activated Cation Channels in Rat Cerebellar Basket Cells J Neurophysiol, October 1, 2000; 84(4): 2026 - 2034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bal and D. Oertel Hyperpolarization-Activated, Mixed-Cation Current (Ih) in Octopus Cells of the Mammalian Cochlear Nucleus J Neurophysiol, August 1, 2000; 84(2): 806 - 817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cai, J. McGee, and E. J. Walsh Contributions of Ion Conductances to the Onset Responses of Octopus Cells in the Ventral Cochlear Nucleus: Simulation Results J Neurophysiol, January 1, 2000; 83(1): 301 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Fitzgerald and D. H. Sanes Serotonergic Modulation of Synapses in the Developing Gerbil Lateral Superior Olive J Neurophysiol, June 1, 1999; 81(6): 2743 - 2752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Bartlett and P. H. Smith Anatomic, Intrinsic, and Synaptic Properties of Dorsal and Ventral Division Neurons in Rat Medial Geniculate Body J Neurophysiol, May 1, 1999; 81(5): 1999 - 2016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. SANTORO and G. R. TIBBS The HCN Gene Family: Molecular Basis of the Hyperpolarization-Activated Pacemaker Channels Ann. N.Y. Acad. Sci., April 30, 1999; 868(1): 741 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Golding, M. J. Ferragamo, and D. Oertel Role of Intrinsic Conductances Underlying Responses to Transients in Octopus Cells of the Cochlear Nucleus J. Neurosci., April 15, 1999; 19(8): 2897 - 2905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Vargas and M. T. Lucero Dopamine Modulates Inwardly Rectifying Hyperpolarization-Activated Current (Ih) in Cultured Rat Olfactory Receptor Neurons J Neurophysiol, January 1, 1999; 81(1): 149 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rathouz and L. Trussell Characterization of Outward Currents in Neurons of the Avian Nucleus Magnocellularis J Neurophysiol, December 1, 1998; 80(6): 2824 - 2835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Magee Dendritic Hyperpolarization-Activated Currents Modify the Integrative Properties of Hippocampal CA1 Pyramidal Neurons J. Neurosci., October 1, 1998; 18(19): 7613 - 7624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang and D. Robertson Substance P-Induced Inward Current in Identified Auditory Efferent Neurons in Rat Brain Stem Slices J Neurophysiol, July 1, 1998; 80(1): 218 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. H. Smith, P. X. Joris, and T. C. T. Yin Anatomy and Physiology of Principal Cells of the Medial Nucleus of the Trapezoid Body (MNTB) of the Cat J Neurophysiol, June 1, 1998; 79(6): 3127 - 3142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Jafri and D. Weinreich Substance P Regulates Ih via a NK-1 Receptor in Vagal Sensory Neurons of the Ferret J Neurophysiol, February 1, 1998; 79(2): 769 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. X. Joris and T. C. T. Yin Envelope Coding in the Lateral Superior Olive. III. Comparison With Afferent Pathways J Neurophysiol, January 1, 1998; 79(1): 253 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-L. Mo and R. L. Davis Heterogeneous Voltage Dependence of Inward Rectifier Currents in Spiral Ganglion Neurons J Neurophysiol, December 1, 1997; 78(6): 3019 - 3027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. W. Fu, B. L. Brezden, and S. H. Wu Hyperpolarization-Activated Inward Current in Neurons of the Rat's Dorsal Nucleus of the Lateral Lemniscus In Vitro J Neurophysiol, November 1, 1997; 78(5): 2235 - 2245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang and D. Robertson Two Types of Actions of Norepinephrine on Identified Auditory Efferent Neurons in Rat Brain Stem Slices J Neurophysiol, October 1, 1997; 78(4): 1800 - 1810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cai, E. J. Walsh, and J. McGee Mechanisms of Onset Responses in Octopus Cells of the Cochlear Nucleus: Implications of a Model J Neurophysiol, August 1, 1997; 78(2): 872 - 883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, R. J. V. Den Berg, and D. L. Ypey Hyperpolarization-Activated Currents in the Growth Cone and Soma of Neonatal Rat Dorsal Root Ganglion Neurons in Culture J Neurophysiol, July 1, 1997; 78(1): 177 - 186. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
