JN Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 70: 2187-2191, 1993;
0022-3077/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Isaacson, J. S.
Right arrow Articles by Nicoll, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Isaacson, J. S.
Right arrow Articles by Nicoll, R. A.

Journal of Neurophysiology, Vol 70, Issue 5 2187-2191, Copyright © 1993 by APS

ARTICLES

The uptake inhibitor L-trans-PDC enhances responses to glutamate but fails to alter the kinetics of excitatory synaptic currents in the hippocampus

J. S. Isaacson and R. A. Nicoll
Physiology Graduate Program, University of California, San Francisco 94143-0450.

1. We have used patch-clamp recording techniques to study the physiological properties of a recently described glutamate uptake blocker, L-trans-pyrrolidine-2,4-dicarboxylic acid (L-trans-PDC), in the CA1 region of the guinea pig hippocampus. 2. L-trans-PDC markedly potentiated the action of exogenously applied glutamate and raised the ambient extracellular levels of glutamate in hippocampal slices. Despite these actions, L-trans-PDC did not affect the time course of either the N-methyl-D-aspartate (NMDA) or non-NMDA receptor-mediated synaptic currents evoked by the stimulation of a large number of neighboring synapses. 3. These findings are consistent with models of fast synaptic transmission in which transmitter is rapidly cleared from the synaptic cleft by diffusion. However, in marked contrast to fast gamma-aminobutyric acid A (GABAA) synapses in the hippocampus, uptake does not appear to play a role in regulating the "spill-over" of transmitter from neighboring, co-activated glutamatergic synapses. Therefore, either diffusion alone can effectively limit the temporal and spatial domain of synaptically released glutamate, or alternatively, L-trans-PDC like other currently available blockers is not sufficiently potent to reveal a role for transmitter uptake at glutamatergic synapses.


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
M. A. Herman and C. E. Jahr
Extracellular Glutamate Concentration in Hippocampal Slice
J. Neurosci., September 5, 2007; 27(36): 9736 - 9741.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Renden, H. Taschenberger, N. Puente, D. A. Rusakov, R. Duvoisin, L.-Y. Wang, K. P. Lehre, and H. von Gersdorff
Glutamate Transporter Studies Reveal the Pruning of Metabotropic Glutamate Receptors and Absence of AMPA Receptor Desensitization at Mature Calyx of Held Synapses
J. Neurosci., September 14, 2005; 25(37): 8482 - 8497.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
Z. Zuo and H. Fang
Glutamate transporter type 3 attenuates the activation of N-methy-D-aspartate receptors co-expressed in Xenopus oocytes
J. Exp. Biol., June 1, 2005; 208(11): 2063 - 2070.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H.-X. Chen and S. N. Roper
Tonic Activity of Metabotropic Glutamate Receptors Is Involved in Developmental Modification of Short-Term Plasticity in the Neocortex
J Neurophysiol, August 1, 2004; 92(2): 838 - 844.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. G. Carter and W. G. Regehr
Prolonged Synaptic Currents and Glutamate Spillover at the Parallel Fiber to Stellate Cell Synapse
J. Neurosci., June 15, 2000; 20(12): 4423 - 4434.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Mennerick, W. Shen, W. Xu, A. Benz, K. Tanaka, K. Shimamoto, K. E. Isenberg, J. E. Krause, and C. F. Zorumski
Substrate Turnover by Transporters Curtails Synaptic Glutamate Transients
J. Neurosci., November 1, 1999; 19(21): 9242 - 9251.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. S. Overstreet, G. A. Kinney, Y.-B. Liu, D. Billups, and N. T. Slater
Glutamate Transporters Contribute to the Time Course of Synaptic Transmission in Cerebellar Granule Cells
J. Neurosci., November 1, 1999; 19(21): 9663 - 9673.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-Y. Min, Z. Melyan, and D. M. Kullmann
Synaptically released glutamate reduces gamma -aminobutyric acid (GABA)ergic inhibition in the hippocampus via kainate receptors
PNAS, August 17, 1999; 96(17): 9932 - 9937.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Matsui, N. Hosoi, and M. Tachibana
Active Role of Glutamate Uptake in the Synaptic Transmission from Retinal Nonspiking Neurons
J. Neurosci., August 15, 1999; 19(16): 6755 - 6766.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. H. Higgs and P. D. Lukasiewicz
Glutamate Uptake Limits Synaptic Excitation of Retinal Ganglion Cells
J. Neurosci., May 15, 1999; 19(10): 3691 - 3700.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Kojima, T. Nakamura, T. Nidaira, K. Nakamura, N. Ooashi, E. Ito, K. Watase, K. Tanaka, K. Wada, Y. Kudo, et al.
Optical Detection of Synaptically Induced Glutamate Transport in Hippocampal Slices
J. Neurosci., April 1, 1999; 19(7): 2580 - 2588.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. PALACIN, R. ESTEVEZ, J. BERTRAN, and A. ZORZANO
Molecular Biology of Mammalian Plasma Membrane Amino Acid Transporters
Physiol Rev, October 1, 1998; 78(4): 969 - 1054.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Bunin and R. M. Wightman
Quantitative Evaluation of 5-Hydroxytryptamine (Serotonin) Neuronal Release and Uptake: An Investigation of Extrasynaptic Transmission
J. Neurosci., July 1, 1998; 18(13): 4854 - 4860.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
F. E. Schweizer, T. Dresbach, W. M. DeBello, V. O'Connor, G. J. Augustine, and H. Betz
Regulation of Neurotransmitter Release Kinetics by NSF
Science, February 20, 1998; 279(5354): 1203 - 1206.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
C. D. Weaver, V. Gundersen, and T. A. Verdoorn
A High Affinity Glutamate/Aspartate Transport System in Pancreatic Islets of Langerhans Modulates Glucose-stimulated Insulin Secretion
J. Biol. Chem., January 16, 1998; 273(3): 1647 - 1653.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Gaal, B. Roska, S. A. Picaud, S. M. Wu, R. Marc, and F. S. Werblin
Postsynaptic Response Kinetics Are Controlled by a Glutamate Transporter at Cone Photoreceptors
J Neurophysiol, January 1, 1998; 79(1): 190 - 196.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. E. Bergles, J. A. Dzubay, and C. E. Jahr
Glutamate transporter currents in Bergmann glial cells follow the time course of extrasynaptic glutamate
PNAS, December 23, 1997; 94(26): 14821 - 14825.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. A. Kinney, L. S. Overstreet, and N. T. Slater
Prolonged Physiological Entrapment of Glutamate in the Synaptic Cleft of Cerebellar Unipolar Brush Cells
J Neurophysiol, September 1, 1997; 78(3): 1320 - 1333.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. S. Diamond and C. E. Jahr
Transporters Buffer Synaptically Released Glutamate on a Submillisecond Time Scale
J. Neurosci., June 15, 1997; 17(12): 4672 - 4687.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Grunewald and B. Kanner
Conformational Changes Monitored on the Glutamate Transporter GLT-1 Indicate the Existence of Two Neurotransmitter-bound States
J. Biol. Chem., July 14, 1995; 270(28): 17017 - 17024.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online