Journal of Neurophysiology, Vol 71, Issue 6 2548-2551, Copyright © 1994 by APS

ARTICLES

Interstitial volume changes during spreading depression (SD) and SD-like hypoxic depolarization in hippocampal tissue slices

J. Jing, P. G. Aitken and G. G. Somjen
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710.

1. Relative interstitial volume (ISV) was estimated from the concentration changes of iontophoretically administered tetramethyl- and tetraethylammonium (TMA+ and TEA+). Spreading depression (SD) was provoked by high K+, and hypoxic SD-like depolarization (HSD) was induced by withdrawing oxygen. 2. Probe ion concentrations increased dramatically and about equally during SD and HSD, except that in a few hypoxic trials signals became transiently smaller than control. Interstitial volume appeared to decrease on the average by approximately 70%. 3. The ISV that remains patent in CA1 region at the height of SD is < 4% of total tissue volume. Probe ions may occasionally have passed through cell membranes for a short time during hypoxic SD.

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