JN Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 72: 349-359, 1994;
0022-3077/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matzner, O.
Right arrow Articles by Devor, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matzner, O.
Right arrow Articles by Devor, M.

Journal of Neurophysiology, Vol 72, Issue 1 349-359, Copyright © 1994 by APS

ARTICLES

Hyperexcitability at sites of nerve injury depends on voltage-sensitive Na+ channels

O. Matzner and M. Devor
Department of Cell and Animal Biology, Hebrew University of Jerusalem, Israel.

1. We used the tested fiber method to record from single myelinated afferents axons ending in a chronic nerve injury site (neuroma) in the rat sciatic nerve or L4,5 dorsal root. Axons were chosen for study that fired spontaneously with a stable tonic or interrupted (bursty) autorhythmic firing pattern. 2. Agents that block voltage-sensitive Na+ channels [tetrodotoxin (TTX), lidocaine], voltage-sensitive Ca2+ channels (Cd2+, Co2+, Ni2+, verapamil, D600, nifedipine, and fluarizine), volt-age-sensitive K+ channels [tetraethylammonium (TEA), 4-aminopyridine (4-AP)], and Ca(2+)-activated K+ channels (gK+Ca2+;quinidine, apamine) were applied topically to the neuroma. Effects on baseline rhythmogenesis and on the duty cycle of bursting were documented. Spike pattern analysis was used to determine whether changes in firing frequency were associated with changes in impulse initiation (electrogenesis), or resulted from (partial) block of impulse propagation downstream from the site of electrogenesis. Effects of veratridine were also noted. 3. Na+ channel blockers consistently quenched neuroma firing, and they did so by suppressing the process of impulse initiation. Only rarely was propagation block the dominant process. In bursty fibers the duration of on-periods shortened as the duration of off-periods lengthened, without a significant change in the baseline interspike interval (ISI). Veratridine accelerated firing, also via the impulse generating process. 4. Ca2+ channel blockers had essentially no effect on baseline firing rate (i.e., ISI). 5. Ca2+ channel blockers, as well as blockers of gK+Ca2+, had substantial, but inconsistent effects on burst pattern. It is not clear whether this reflects variability in the experimental conditions, or heterogeneity among the fibers sampled. 6. Blockade of K+ channels failed to evoke rhythmogenesis in acutely cut axons as it does in chronically injured axons, even in the presence of veratridine. This is consistent with other evidence that ectopic neuroma firing depends on postinjury remodeling of membrane electrical properties. 7. The data indicate that, in chronically injured axons, the inward currents that underly electrogenicity, enable ectopic discharge, and, together with outward K+ currents, set the fundamental firing rhythm (ISI), operate primarily with the use of voltage-sensitive Na+ rather than Ca2+ channels. 8. The on-off duty cycle in bursty fibers was affected by Na+ channel ligands and also, although less so, and less consistently by, Ca2+ channel ligands. This indicates that both may play a role in the slow modulations of membrane potential that presumably underly interrupted autorhythmicity.


This article has been cited by other articles:


Home page
 Anesth. Analg.Home page
J. W. Shin, C. Pancaro, C. F. Wang, and P. Gerner
Low-Dose Systemic Bupivacaine Prevents the Development of Allodynia After Thoracotomy in Rats
Anesth. Analg., November 1, 2008; 107(5): 1587 - 1591.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
A. Dray
Neuropathic pain: emerging treatments
Br. J. Anaesth., July 1, 2008; 101(1): 48 - 58.
[Abstract] [Full Text] [PDF]

Home page
 Arch NeurolHome page
M. C. Franca Jr, A. D'Abreu, A. Nucci, and I. Lopes-Cendes
Muscle Excitability Abnormalities in Machado-Joseph Disease
Arch Neurol, April 1, 2008; 65(4): 525 - 529.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
R. Benoliel, J. Epstein, E. Eliav, R. Jurevic, and S. Elad
Orofacial Pain in Cancer: Part I--Mechanisms
J. Dent. Res., June 1, 2007; 86(6): 491 - 505.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. M. Rush, T. R. Cummins, and S. G. Waxman
Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons
J. Physiol., February 15, 2007; 579(1): 1 - 14.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. R. Cummins, S. D. Dib-Hajj, and S. G. Waxman
Electrophysiological Properties of Mutant Nav1.7 Sodium Channels in a Painful Inherited Neuropathy
J. Neurosci., September 22, 2004; 24(38): 8232 - 8236.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. S. Shah, A. M. Rush, S. Liu, L. Tyrrell, J. A. Black, S. D. Dib-Hajj, and S. G. Waxman
Contactin Associates with Sodium Channel Nav1.3 in Native Tissues and Increases Channel Density at the Cell Surface
J. Neurosci., August 18, 2004; 24(33): 7387 - 7399.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. C. Hains, C. Y. Saab, J. P. Klein, M. J. Craner, and S. G. Waxman
Altered Sodium Channel Expression in Second-Order Spinal Sensory Neurons Contributes to Pain after Peripheral Nerve Injury
J. Neurosci., May 19, 2004; 24(20): 4832 - 4839.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. C. J. Wallace, D. F. Cottrell, P. J. Brophy, and S. M. Fleetwood-Walker
Focal Lysolecithin-Induced Demyelination of Peripheral Afferents Results in Neuropathic Pain Behavior That Is Attenuated by Cannabinoids
J. Neurosci., April 15, 2003; 23(8): 3221 - 3233.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
K. Kanai, S. Kuwabara, K. Arai, J.-Y. Sung, K. Ogawara, and T. Hattori
Muscle cramp in Machado-Joseph disease: Altered motor axonal excitability properties and mexiletine treatment
Brain, April 1, 2003; 126(4): 965 - 973.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H.-L. Pan, G. M. Khan, K. D. Alloway, and S.-R. Chen
Resiniferatoxin Induces Paradoxical Changes in Thermal and Mechanical Sensitivities in Rats: Mechanism of Action
J. Neurosci., April 1, 2003; 23(7): 2911 - 2919.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. R. Chaplan, H.-Q. Guo, D. H. Lee, L. Luo, C. Liu, C. Kuei, A. A. Velumian, M. P. Butler, S. M. Brown, and A. E. Dubin
Neuronal Hyperpolarization-Activated Pacemaker Channels Drive Neuropathic Pain
J. Neurosci., February 15, 2003; 23(4): 1169 - 1178.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Barclay, S. Patel, G. Dorn, G. Wotherspoon, S. Moffatt, L. Eunson, S. Abdel'al, F. Natt, J. Hall, J. Winter, et al.
Functional Downregulation of P2X3 Receptor Subunit in Rat Sensory Neurons Reveals a Significant Role in Chronic Neuropathic and Inflammatory Pain
J. Neurosci., September 15, 2002; 22(18): 8139 - 8147.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S.-R. Chen, K. M. Wozniak, B. S. Slusher, and H.-L. Pan
Effect of 2-(Phosphono-methyl)-pentanedioic Acid on Allodynia and Afferent Ectopic Discharges in a Rat Model of Neuropathic Pain
J. Pharmacol. Exp. Ther., February 1, 2002; 300(2): 662 - 667.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
D. Bridges, S. W. N. Thompson, and A. S. C. Rice
Mechanisms of neuropathic pain
Br. J. Anaesth., July 1, 2001; 87(1): 12 - 26.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Amir, M. Michaelis, and M. Devor
Membrane Potential Oscillations in Dorsal Root Ganglion Neurons: Role in Normal Electrogenesis and Neuropathic Pain
J. Neurosci., October 1, 1999; 19(19): 8589 - 8596.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. G. Waxman, S. Dib-Hajj, T. R. Cummins, and J. A. Black
Sodium channels and pain
PNAS, July 6, 1999; 96(14): 7635 - 7639.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Porreca, J. Lai, D. Bian, S. Wegert, M. H. Ossipov, R. M. Eglen, L. Kassotakis, S. Novakovic, D. K. Rabert, L. Sangameswaran, et al.
A comparison of the potential role of the tetrodotoxin-insensitive sodium channels, PN3/SNS and NaN/SNS2, in rat models of chronic pain
PNAS, July 6, 1999; 96(14): 7640 - 7644.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
H.-L. Pan, J. C. Eisenach, and S.-R. Chen
Gabapentin Suppresses Ectopic Nerve Discharges and Reverses Allodynia in Neuropathic Rats
J. Pharmacol. Exp. Ther., March 1, 1999; 288(3): 1026 - 1030.
[Abstract] [Full Text]

Home page
 J. Neurophysiol.Home page
J. Fjell, T. R. Cummins, K. Fried, J. A. Black, and S. G. Waxman
In Vivo NGF Deprivation Reduces SNS Expression and TTX-R Sodium Currents in IB4-Negative DRG Neurons
J Neurophysiol, February 1, 1999; 81(2): 803 - 810.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. D. Novakovic, E. Tzoumaka, J. G. McGivern, M. Haraguchi, L. Sangameswaran, K. R. Gogas, R. M. Eglen, and J. C. Hunter
Distribution of the Tetrodotoxin-Resistant Sodium Channel PN3 in Rat Sensory Neurons in Normal and Neuropathic Conditions
J. Neurosci., March 15, 1998; 18(6): 2174 - 2187.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. R. Cummins and S. G. Waxman
Downregulation of Tetrodotoxin-Resistant Sodium Currents and Upregulation of a Rapidly Repriming Tetrodotoxin-Sensitive Sodium Current in Small Spinal Sensory Neurons after Nerve Injury
J. Neurosci., May 15, 1997; 17(10): 3503 - 3514.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
M. Devor
REVIEW {blacksquare} : Pain Mechanisms
Neuroscientist, July 1, 1996; 2(4): 233 - 244.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online