| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Neurophysiology, Vol 72, Issue 4 1549-1560, Copyright © 1994 by APS
ARTICLES |
Y. Zhu and S. R. Ikeda
Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta 30912-2300.
1. Modulation of Ca(2+)-channel currents by phorbol-12-myristate-13-acetate (PMA) was investigated in acutely dissociated adult rat superior cervical ganglion neurons using the whole cell variant of the patch-clamp technique. 2. PMA (500 nM) increased the current amplitudes, accelerated the inactivation of step currents, retarded the deactivation of tail currents, and shifted the tail current activation to more negative potentials. 3. The effects of PMA were concentration and voltage dependent and mediated through activation of protein kinase C (PKC). PMA also increased Ca2+ currents recorded with the perforated patch technique. 4. PMA affected the N-type Ca2+ channels and an omega-conotoxin GVIA-resistant current component. Ca2+ currents affected by PMA were not sensitive to omega-agatoxin IVA or nimodipine. 5. PMA not only attenuated Ca(2+)-channel inhibition induced by alpha 2-adrenoceptor agonist, which modulates Ca2+ channels via a pertussis toxin (PTX)-sensitive pathway, but also attenuated current inhibition by vasoactive intestinal polypeptide, which modulates Ca2+ channels via a PTX-insensitive but cholera toxin-sensitive pathway. 6. PMA reversed Ca(2+)-channel inhibition induced by tonic activation of G-protein in the absence of neurotransmitter (even in neurons pretreated with PTX) or induced by activation of G-proteins with guanosine 5'-O-(3-thiotriphosphate) (GTP)-gamma-S. 7. Inhibition of phosphatase by okadaic acid or substitution of Ba2+ for Ca2+ in the external solutions accelerated the PMA effect. 8. Our results suggest that activation of PKC antagonizes G-protein mediated inhibition of Ca2+ channels by shifting Ca2+ channels from the "reluctant" state to the "willing" state. The G-proteins and, more likely, the N-type Ca2+ channels may be the target of PKC phosphorylation. Protein phosphatases may be involved in counteracting the PKC phosphorylation in rat sympathetic neurons.
This article has been cited by other articles:
|
|
 |
|
  C. S. Bauer, R. J. Woolley, A. G. Teschemacher, and E. P. Seward Potentiation of Exocytosis by Phospholipase C-Coupled G-Protein-Coupled Receptors Requires the Priming Protein Munc13-1 J. Neurosci., January 3, 2007; 27(1): 212 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Strock and M. A. Diverse-Pierluissi Ca2+ Channels As Integrators of G Protein-Mediated Signaling in Neurons Mol. Pharmacol., November 1, 2004; 66(5): 1071 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Bannister, K. Melliti, and B. A. Adams Differential Modulation of CaV2.3 Ca2+ Channels by G{alpha}q/11-Coupled Muscarinic Receptors Mol. Pharmacol., February 1, 2004; 65(2): 381 - 388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Kammermeier, M. I. Davis, and S. R. Ikeda Specificity of Metabotropic Glutamate Receptor 2 Coupling to G Proteins Mol. Pharmacol., January 1, 2003; 63(1): 183 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Simen, C. C. Lee, B. B. Simen, V. P. Bindokas, and R. J. Miller The C Terminus of the Ca Channel {alpha}1B Subunit Mediates Selective Inhibition by G-Protein-Coupled Receptors J. Neurosci., October 1, 2001; 21(19): 7587 - 7597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Lu, M. S. AtKisson, S. E. Jarvis, Z.-P. Feng, G. W. Zamponi, and K. Dunlap Syntaxin 1A Supports Voltage-Dependent Inhibition of {alpha}1B Ca2+ Channels by G{beta}{gamma} in Chick Sensory Neurons J. Neurosci., May 1, 2001; 21(9): 2949 - 2957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Kammermeier, V. Ruiz-Velasco, and S. R. Ikeda A Voltage-Independent Calcium Current Inhibitory Pathway Activated by Muscarinic Agonists in Rat Sympathetic Neurons Requires Both Galpha q/11 and Gbeta gamma J. Neurosci., August 1, 2000; 20(15): 5623 - 5629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Haley, P. Delmas, S. Offermanns, F. C. Abogadie, M. I. Simon, N. J. Buckley, and D. A. Brown Muscarinic Inhibition of Calcium Current and M Current in Galpha q-Deficient Mice J. Neurosci., June 1, 2000; 20(11): 3973 - 3979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-J. Song, T. Tkatch, and D. J. Surmeier Adenosine Receptor Expression and Modulation of Ca2+ Channels in Rat Striatal Cholinergic Interneurons J Neurophysiol, January 1, 2000; 83(1): 322 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Herzig and J. Neumann Effects of Serine/Threonine Protein Phosphatases on Ion Channels in Excitable Membranes Physiol Rev, January 1, 2000; 80(1): 173 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhang and J. T. Schmidt Adenosine A1 and Class II Metabotropic Glutamate Receptors Mediate Shared Presynaptic Inhibition of Retinotectal Transmission J Neurophysiol, December 1, 1999; 82(6): 2947 - 2955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-Q. Sun and N. Dale G-Proteins Are Involved in 5-HT Receptor-Mediated Modulation of N- and P/Q- But Not T-Type Ca2+ Channels J. Neurosci., February 1, 1999; 19(3): 890 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lu, H. Yang, R. H. Lenox, and M. K. Raizada Regulation of Angiotensin II-induced Neuromodulation by MARCKS in Brain Neurons J. Cell Biol., July 13, 1998; 142(1): 217 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. McCullough, T. M. Egan, and T. C. Westfall Neuropeptide Y inhibition of calcium channels in PC-12 pheochromocytoma cells Am J Physiol Cell Physiol, May 1, 1998; 274(5): C1290 - C1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Garcia, S. Brown, B. Hille, and K. Mackie Protein Kinase C Disrupts Cannabinoid Actions by Phosphorylation of the CB1 Cannabinoid Receptor J. Neurosci., April 15, 1998; 18(8): 2834 - 2841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Nair, T. R. Werkman, J. Craig, R. Finnell, M. Joels, and J. H. Eberwine Corticosteroid Regulation of Ion Channel Conductances and mRNA Levels in Individual Hippocampal CA1 Neurons J. Neurosci., April 1, 1998; 18(7): 2685 - 2696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Kammermeier and S. W. Jones Facilitation of L-type Calcium Current in Thalamic Neurons J Neurophysiol, January 1, 1998; 79(1): 410 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu and J. L. Yakel Modulation of Ca2+ Currents by Various G Protein-Coupled Receptors in Sympathetic Neurons of Male Rat Pelvic Ganglia J Neurophysiol, August 1, 1997; 78(2): 780 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu and J. L. Yakel Calcineurin Modulates G Protein-Mediated Inhibition of N-Type Calcium Channels in Rat Sympathetic Neurons J Neurophysiol, August 1, 1997; 78(2): 1161 - 1169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen and N. J. Penington Order of Application Determines the Interaction Between Phorbol Esters and GTP-gamma -S in Dorsal Raphe Neurons: Evidence That the Effect of 5-HT Is Modified Upstream of the G Protein Ca Channel Interaction J Neurophysiol, May 1, 1997; 77(5): 2697 - 2703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Yan, W.-J. Song, and D. J. Surmeier D2 Dopamine Receptors Reduce N-Type Ca2+ Currents in Rat Neostriatal Cholinergic Interneurons Through a Membrane-Delimited, Protein-Kinase-C-Insensitive Pathway J Neurophysiol, February 1, 1997; 77(2): 1003 - 1015. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
