| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Neurophysiology, Vol 73, Issue 1 320-332, Copyright © 1995 by APS
ARTICLES |
S. Mennerick, J. Que, A. Benz and C. F. Zorumski
Department of Psychiatry, Washington University School of Medicine, St Louis, Missouri 63110.
1. We used whole cell recordings to compare passive membrane properties and synaptic properties of postnatal rat hippocampal neurons grown for 7-15 days in either conventional mass cultures or on physically restricted microisland cultures. Despite matching microisland and mass culture cell across several variables, there were significant differences between neurons in the two groups regarding passive membrane characteristics and synaptic properties. 2. Microisland neurons displayed significantly faster charging of the membrane capacitance than mass culture counterparts matched with microisland neurons for age, somal diameter, and transmitter phenotype. When we used a two-compartment equivalent circuit model to quantify this result, microisland neurons displayed approximately half the distal capacitance of mass culture neurons. These data suggest that microisland neurons elaborate less extensive neuritic arborizations than mass culture neurons. 3. Evoked synaptic responses were enhanced on microislands compared with mass cultures. Excitatory and inhibitory autaptic currents were more frequent and displayed larger amplitudes on single-neuron microislands than in matched mass culture neurons. 4. In recordings from pairs of neurons in the two environments, we observed a significantly higher probability of obtaining a monosynaptic response on two-neuron microislands than in matched mass culture pairs (85% vs. 42%). Evoked excitatory postsynaptic currents were also significantly larger in the microisland environment, with evoked excitatory synaptic currents from two-neuron microislands exhibiting a mean amplitude 20-fold larger than mass culture monosynaptic responses. 5. The differences in evoked synaptic responses were not reflected in differences in the amplitude or frequency of spontaneous miniature excitatory postsynaptic currents (mEPSCs). Analysis of mEPSC rise times, decay times, and peak amplitudes within individual cells suggests that electrotonic filtering is not an important contributor to the variability of peak amplitudes and decay times of synaptic currents in cells of either culture environment. However, composite data across neurons in both cultures reveal a significant correlation between mEPSC rise and decay times. 6. Out results suggest that the microisland preparation may be a useful tool for exploring factors that influence synapse formation and development. Additionally, the preparation is a particularly convenient model for the study of single-neuron-mediated synaptic events.
This article has been cited by other articles:
|
|
 |
|
  K. L. Moulder, X. Jiang, C. Chang, A. A. Taylor, A. M. Benz, A. C. Conti, L. J. Muglia, and S. Mennerick A Specific Role for Ca2+-Dependent Adenylyl Cyclases in Recovery from Adaptive Presynaptic Silencing J. Neurosci., May 14, 2008; 28(20): 5159 - 5168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Cho and C. C. Askwith Presynaptic Release Probability Is Increased in Hippocampal Neurons From ASIC1 Knockout Mice J Neurophysiol, February 1, 2008; 99(2): 426 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Moulder, X. Jiang, A. A. Taylor, W. Shin, K. D. Gillis, and S. Mennerick Vesicle Pool Heterogeneity at Hippocampal Glutamate and GABA Synapses J. Neurosci., September 12, 2007; 27(37): 9846 - 9854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Steinmetz, I. Buard, T. Claudepierre, K. Nagler, and F. W. Pfrieger Regional variations in the glial influence on synapse development in the mouse CNS J. Physiol., November 15, 2006; 577(1): 249 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Moulder, X. Jiang, A. A. Taylor, J. W. Olney, and S. Mennerick Physiological activity depresses synaptic function through an effect on vesicle priming. J. Neurosci., June 14, 2006; 26(24): 6618 - 6626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G.J. Allen, F. C. Abogadie, and D. A. Brown Simultaneous Release of Glutamate and Acetylcholine from Single Magnocellular "Cholinergic" Basal Forebrain Neurons J. Neurosci., February 1, 2006; 26(5): 1588 - 1595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Moulder and S. Mennerick Reluctant Vesicles Contribute to the Total Readily Releasable Pool in Glutamatergic Hippocampal Neurons J. Neurosci., April 13, 2005; 25(15): 3842 - 3850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Shu, L. N. Eisenman, D. Jinadasa, D. F. Covey, C. F. Zorumski, and S. Mennerick Slow Actions of Neuroactive Steroids at GABAA Receptors J. Neurosci., July 28, 2004; 24(30): 6667 - 6675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mennerick, Y. He, X. Jiang, B. D. Manion, M. Wang, A. Shute, A. Benz, A. S. Evers, D. F. Covey, and C. F. Zorumski Selective Antagonism of 5{alpha}-Reduced Neurosteroid Effects at GABAA Receptors Mol. Pharmacol., May 1, 2004; 65(5): 1191 - 1197. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. C. Askwith, J. A. Wemmie, M. P. Price, T. Rokhlina, and M. J. Welsh Acid-sensing Ion Channel 2 (ASIC2) Modulates ASIC1 H+-activated Currents in Hippocampal Neurons J. Biol. Chem., April 30, 2004; 279(18): 18296 - 18305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Yermolaieva, A. S. Leonard, M. K. Schnizler, F. M. Abboud, and M. J. Welsh Extracellular acidosis increases neuronal cell calcium by activating acid-sensing ion channel 1a PNAS, April 27, 2004; 101(17): 6752 - 6757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Darbandi-Tonkabon, B. D. Manion, W. R. Hastings, W. J. Craigen, G. Akk, J. R. Bracamontes, Y. He, T. V. Sheiko, J. H. Steinbach, S. J. Mennerick, et al. Neuroactive Steroid Interactions with Voltage-Dependent Anion Channels: Lack of Relationship to GABAA Receptor Modulation and Anesthesia J. Pharmacol. Exp. Ther., February 1, 2004; 308(2): 502 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Moulder, R. J. Cormier, A. A. Shute, C. F. Zorumski, and S. Mennerick Homeostatic Effects of Depolarization on Ca2+ Influx, Synaptic Signaling, and Survival J. Neurosci., March 1, 2003; 23(5): 1825 - 1831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bacci, J. R. Huguenard, and D. A. Prince Functional Autaptic Neurotransmission in Fast-Spiking Interneurons: A Novel Form of Feedback Inhibition in the Neocortex J. Neurosci., February 1, 2003; 23(3): 859 - 866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wang, Y. He, L. N. Eisenman, C. Fields, C.-M. Zeng, J. Mathews, A. Benz, T. Fu, E. Zorumski, J. H. Steinbach, et al. 3beta -Hydroxypregnane Steroids Are Pregnenolone Sulfate-Like GABAA Receptor Antagonists J. Neurosci., May 1, 2002; 22(9): 3366 - 3375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. He, C. F. Zorumski, and S. Mennerick Contribution of Presynaptic Na+ Channel Inactivation to Paired-Pulse Synaptic Depression in Cultured Hippocampal Neurons J Neurophysiol, February 1, 2002; 87(2): 925 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mennerick, C.-M. Zeng, A. Benz, W. Shen, Y. Izumi, A. S. Evers, D. F. Covey, and C. F. Zorumski Effects on gamma -Aminobutyric Acid (GABA)A Receptors of a Neuroactive Steroid That Negatively Modulates Glutamate Neurotransmission and Augments GABA Neurotransmission Mol. Pharmacol., October 1, 2001; 60(4): 732 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nadeau, S. McKinney, D. J. Anderson, and H. A. Lester ROMK1 (Kir1.1) Causes Apoptosis and Chronic Silencing of Hippocampal Neurons J Neurophysiol, August 1, 2000; 84(2): 1062 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Shen, S. Mennerick, D. F. Covey, and C. F. Zorumski Pregnenolone Sulfate Modulates Inhibitory Synaptic Transmission by Enhancing GABAA Receptor Desensitization J. Neurosci., May 15, 2000; 20(10): 3571 - 3579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Bolton, A. J. Pittman, and D. C. Lo Brain-Derived Neurotrophic Factor Differentially Regulates Excitatory and Inhibitory Synaptic Transmission in Hippocampal Cultures J. Neurosci., May 1, 2000; 20(9): 3221 - 3232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mennerick, W. Shen, W. Xu, A. Benz, K. Tanaka, K. Shimamoto, K. E. Isenberg, J. E. Krause, and C. F. Zorumski Substrate Turnover by Transporters Curtails Synaptic Glutamate Transients J. Neurosci., November 1, 1999; 19(21): 9242 - 9251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. T. Sherwood and D. C. Lo Long-Term Enhancement of Central Synaptic Transmission by Chronic Brain-Derived Neurotrophic Factor Treatment J. Neurosci., August 15, 1999; 19(16): 7025 - 7036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mennerick, V. Jevtovic-Todorovic, S. M. Todorovic, W. Shen, J. W. Olney, and C. F. Zorumski Effect of Nitrous Oxide on Excitatory and Inhibitory Synaptic Transmission in Hippocampal Cultures J. Neurosci., December 1, 1998; 18(23): 9716 - 9726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Todorovic, M. Prakriya, Y. M. Nakashima, K. R. Nilsson, M. Han, C. F. Zorumski, D. F. Covey, and C. J. Lingle Enantioselective Blockade of T-type Ca2+ Current in Adult Rat Sensory Neurons by a Steroid That Lacks gamma -Aminobutyric Acid-Modulatory Activity Mol. Pharmacol., November 1, 1998; 54(5): 918 - 927. [Abstract] [Full Text]
|
|
|
|
|
|
Y. M. Nakashima, S. M. Todorovic, D. F. Covey, and C. J. Lingle The Anesthetic Steroid (+)-3alpha -Hydroxy-5alpha -androstane-17beta -carbonitrile Blocks N-, Q-, and R-Type, but Not L- and P-Type, High Voltage-Activated Ca2+ Current in Hippocampal and Dorsal Root Ganglion Neurons of the Rat Mol. Pharmacol., September 1, 1998; 54(3): 559 - 568. [Abstract] [Full Text]
|
|
|
|
|
|
M. W. Hill, P. A. Reddy, D. F. Covey, and S. M. Rothman Contribution of Subsaturating GABA Concentrations to IPSCs in Cultured Hippocampal Neurons J. Neurosci., July 15, 1998; 18(14): 5103 - 5111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mennerick, R. P. Dhond, A. Benz, W. Xu, J. D. Rothstein, N. C. Danbolt, K. E. Isenberg, and C. F. Zorumski Neuronal Expression of the Glutamate Transporter GLT-1 in Hippocampal Microcultures J. Neurosci., June 15, 1998; 18(12): 4490 - 4499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-P. Feng, J. Klumperman, K. Lukowiak, and N. I. Syed In Vitro Synaptogenesis between the Somata of Identified Lymnaea Neurons Requires Protein Synthesis But Not Extrinsic Growth Factors or Substrate Adhesion Molecules J. Neurosci., October 15, 1997; 17(20): 7839 - 7849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mennerick, D. Zenisek, and G. Matthews Static and Dynamic Membrane Properties of Large-Terminal Bipolar Cells From Goldfish Retina: Experimental Test of a Compartment Model J Neurophysiol, July 1, 1997; 78(1): 51 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kimura, Y. Otsu, and T. Tsumoto Presynaptically Silent Synapses: Spontaneously Active Terminals Without Stimulus-Evoked Release Demonstrated in Cortical Autapses J Neurophysiol, May 1, 1997; 77(5): 2805 - 2815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Gubitosi-Klug and R. W. Gross Fatty Acid Ethyl Esters, Nonoxidative Metabolites of Ethanol, Accelerate the Kinetics of Activation of the Human Brain Delayed Rectifier K+ Channel, Kv1.1 J. Biol. Chem., December 20, 1996; 271(51): 32519 - 32522. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
