Journal of Neurophysiology, Vol 74, Issue 3 1355-1357, Copyright © 1995 by APS

ARTICLES

Deafferentation increases the intracellular calcium of cochlear nucleus neurons in the embryonic chick

L. Zirpel, E. A. Lachica and W. R. Lippe
Department of Physiology and Biophysics, SJ-40, School of Medicine, University of Washington, Seattle 98195, USA.

1. Ratiometric fura-2 imaging was used to measure the intracellular calcium concentration ([Ca2+]i) of neurons in the embryonic avian cochlear nucleus, nucleus magnocellularis (NM), after an in ovo unilateral cochlea removal (deafferentation). 2. The mean [Ca2+]i of NM neurons receiving normal input was 113 nM. 3. Deafferentation increased the mean [Ca2+]i of NM neurons to 247, 311, 339, and 314 nM at 1, 3, 6, and 12 h after cochlear removal, respectively. These values did not differ significantly. 4. The percent frequency distribution of deafferented NM neuron [Ca2+]i shifts away from normative levels toward higher [Ca2+]i at 1 and 3 h after cochlear removal, but shifts back toward normative levels at 6 and 12 h after cochlear removal. 5. This increased [Ca2+]i following cochlear removal temporally coincides with well-characterized changes in NM neurons following activity deprivation. 6. These data suggest that deregulation of [Ca2+]i homeostasis plays a key role in NM neuron degeneration and death following activity deprivation.

This article has been cited by other articles:

				J. I. Luoma and L. Zirpel Deafferentation-Induced Activation of NFAT (Nuclear Factor of Activated T-Cells) in Cochlear Nucleus Neurons during a Developmental Critical Period: A Role for NFATc4-Dependent Apoptosis in the CNS J. Neurosci., March 19, 2008; 28(12): 3159 - 3169. [Abstract] [Full Text] [PDF]

				D. Eugene, S. Deforges, F. Guimont, E. Idoux, P.-P. Vidal, L. E. Moore, and N. Vibert Developmental regulation of the membrane properties of central vestibular neurons by sensory vestibular information in the mouse J. Physiol., September 15, 2007; 583(3): 923 - 943. [Abstract] [Full Text] [PDF]

				Y. Lu and E. W Rubel Activation of Metabotropic Glutamate Receptors Inhibits High-Voltage-Gated Calcium Channel Currents of Chicken Nucleus Magnocellularis Neurons J Neurophysiol, March 1, 2005; 93(3): 1418 - 1428. [Abstract] [Full Text] [PDF]

				L. Zirpel, M. A. Janowiak, C. A. Veltri, and T. N. Parks AMPA Receptor-Mediated, Calcium-Dependent CREB Phosphorylation in a Subpopulation of Auditory Neurons Surviving Activity Deprivation J. Neurosci., August 15, 2000; 20(16): 6267 - 6275. [Abstract] [Full Text] [PDF]

				S. P. Mostafapour, D. M. Hockenbery, and E. W Rubel Life and Death in Otolaryngology: Mechanisms of Apoptosis and Its Role in the Pathology and Treatment of Disease Arch Otolaryngol Head Neck Surg, July 1, 1999; 125(7): 729 - 737. [Abstract] [Full Text] [PDF]

				B. M. Kato and E. W Rubel Glutamate Regulates IP3-Type and CICR Stores in the Avian Cochlear Nucleus J Neurophysiol, April 1, 1999; 81(4): 1587 - 1596. [Abstract] [Full Text] [PDF]

				L. Zirpel, W. R. Lippe, and E. W Rubel Activity-Dependent Regulation of [Ca2+]i in Avian Cochlear Nucleus Neurons: Roles of Protein Kinases A and C and Relation to Cell Death J Neurophysiol, May 1, 1998; 79(5): 2288 - 2302. [Abstract] [Full Text] [PDF]

				S. P. Mostafapour, E. A. Lachica, and E. W Rubel Mitochondrial Regulation of Calcium in the Avian Cochlear Nucleus J Neurophysiol, October 1, 1997; 78(4): 1928 - 1934. [Abstract] [Full Text] [PDF]

				J. L. Hegarty, A. R. Kay, and S. H. Green Trophic Support of Cultured Spiral Ganglion Neurons by Depolarization Exceeds and Is Additive with that by Neurotrophins or cAMP and Requires Elevation of [Ca2+]i within a Set Range J. Neurosci., March 15, 1997; 17(6): 1959 - 1970. [Abstract] [Full Text] [PDF]

				T. Otis, S. Zhang, and L. O. Trussell Direct Measurement of AMPA Receptor Desensitization Induced by Glutamatergic Synaptic Transmission J. Neurosci., December 1, 1996; 16(23): 7496 - 7504. [Abstract] [Full Text] [PDF]