| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Neurophysiology, Vol 74, Issue 3 990-1000, Copyright © 1995 by APS
ARTICLES |
C. L. Cox, J. R. Huguenard and D. A. Prince
Department of Neurology and Neurological Sciences, Stanford University Medical Center, California 94305, USA.
1. The thalamic reticular nucleus (nRt) is innervated by cholecystokinin (CCK)-containing neurons and contains CCK binding sites. We used tight-seal, whole cell recording techniques with in vitro rat thalamic slices to investigate the action of CCK on neurons in nRt and ventrobasal thalamus (VB). 2. Brief applications of the CCK agonist cholecystokinin octapeptide (26-33) sulfated (CCK8S) evoked prolonged spike discharges in nRt neurons but had no direct effects on VB neuron activity. This selective excitatory action of CCK8S in nRt resulted from a long-lasting membrane depolarization (2-10 min) associated with an increased input resistance. Voltage-clamp recordings revealed that CCK8S reduced membrane conductance by 0.6-3.8 nS, which amounted to 5-54% of the resting conductance of these neurons. 3. The conductance blocked by CCK8S was linear over the range of -50 to -100 mV and reversed near the potassium equilibrium potential. Modifications of extracellular K+ concentration altered the reversal potential of the conductance as predicted by the Nernst equation. The K+ channel blocker Cs+, applied either intracellularly or combined intra- and extracellularly, blocked the response to CCK8S. 4. The CCK8S-induced depolarization persisted after suppression of synaptic transmission by either tetrodotoxin or a low-Ca2+, high-Mg2+ extracellular solution, indicating that the depolarization was primarily due to activation of postsynaptic CCK receptors and not mediated through the release of other neurotransmitters. 5. The selective CCKA antagonists L364,718 and Cam-1481 attenuated the CCK8S-induced depolarization, whereas the CCKB antagonist L365,260 had little or no effect on the depolarization. 6. Our findings indicate that CCK8S, acting via CCKA-type receptors, reduces a K+ leak current, resulting in a long-lasting membrane depolarization that can presumably modify the firing mode of nRt neurons. Through this effect, CCK actions in nRt may strongly influence thalamocortical function.
This article has been cited by other articles:
|
|
 |
|
  L. Chung and S. D. Moore Cholecystokinin Excites Interneurons in Rat Basolateral Amygdala J Neurophysiol, July 1, 2009; 102(1): 272 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Holmes, M. Tong, and R. A. Travagli Effects of brain stem cholecystokinin-8s on gastric tone and esophageal-gastric reflex Am J Physiol Gastrointest Liver Physiol, March 1, 2009; 296(3): G621 - G631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yang and C. L. Cox Excitatory and anti-oscillatory actions of nitric oxide in thalamus J. Physiol., August 1, 2008; 586(15): 3617 - 3628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Oz, K.-H. Yang, T. S. Shippenberg, L. P. Renaud, and M. J. O'Donovan Cholecystokinin B-Type Receptors Mediate a G-Protein-Dependent Depolarizing Action of Sulphated Cholecystokinin Ocatapeptide (CCK-8s) on Rodent Neonatal Spinal Ventral Horn Neurons J Neurophysiol, September 1, 2007; 98(3): 1108 - 1114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wan, F. H. Coleman, and R. A. Travagli Cholecystokinin-8s excites identified rat pancreatic-projecting vagal motoneurons Am J Physiol Gastrointest Liver Physiol, August 1, 2007; 293(2): G484 - G492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Lee and C. L. Cox Excitatory Actions of Vasoactive Intestinal Peptide on Mouse Thalamocortical Neurons Are Mediated by VPAC2 Receptors J Neurophysiol, August 1, 2006; 96(2): 858 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Pinault, A. Slezia, and L. Acsady Corticothalamic 5-9 Hz oscillations are more pro-epileptogenic than sleep spindles in rats J. Physiol., July 1, 2006; 574(1): 209 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-Y. Deng and S. Lei Bidirectional modulation of GABAergic transmission by cholecystokinin in hippocampal dentate gyrus granule cells of juvenile rats J. Physiol., April 15, 2006; 572(2): 425 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zheng, M. W. Lewis, and R. A. Travagli In vitro analysis of the effects of cholecystokinin on rat brain stem motoneurons Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1066 - G1073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Broberger and D. A. McCormick Excitatory Effects of Thyrotropin-Releasing Hormone in the Thalamus J. Neurosci., February 16, 2005; 25(7): 1664 - 1673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Lee and C. L. Cox Vasoactive Intestinal Peptide Selectively Depolarizes Thalamic Relay Neurons and Attenuates Intrathalamic Rhythmic Activity J Neurophysiol, August 1, 2003; 90(2): 1224 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-Q. Sun, D. A. Prince, and J. R. Huguenard Vasoactive Intestinal Polypeptide and Pituitary Adenylate Cyclase-Activating Polypeptide Activate Hyperpolarization-Activated Cationic Current and Depolarize Thalamocortical Neurons In Vitro J. Neurosci., April 1, 2003; 23(7): 2751 - 2758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Herness, F.-l. Zhao, S.-g. Lu, N. Kaya, and T. Shen Expression and Physiological Actions of Cholecystokinin in Rat Taste Receptor Cells J. Neurosci., November 15, 2002; 22(22): 10018 - 10029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-Q. Sun, J. R. Huguenard, and D. A. Prince Somatostatin Inhibits Thalamic Network Oscillations In Vitro: Actions on the GABAergic Neurons of the Reticular Nucleus J. Neurosci., July 1, 2002; 22(13): 5374 - 5386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. O. Lankisch, Y. Tsunoda, Y. Lu, and C. Owyang Characterization of CCKA receptor affinity states and Ca2+ signal transduction in vagal nodose ganglia Am J Physiol Gastrointest Liver Physiol, June 1, 2002; 282(6): G1002 - G1008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Meis, T. Munsch, and H.-C. Pape Antioscillatory Effects of Nociceptin/Orphanin FQ in Synaptic Networks of the Rat Thalamus J. Neurosci., February 1, 2002; 22(3): 718 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Cooper, E. Harrington, Y. N. Jan, and L. Y. Jan M Channel KCNQ2 Subunits Are Localized to Key Sites for Control of Neuronal Network Oscillations and Synchronization in Mouse Brain J. Neurosci., December 15, 2001; 21(24): 9529 - 9540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Browne, J. Kang, G. Akk, L. W. Chiang, H. Schulman, J. R. Huguenard, and D. A. Prince Kinetic and Pharmacological Properties of GABAA Receptors in Single Thalamic Neurons and GABAA Subunit Expression J Neurophysiol, November 1, 2001; 86(5): 2312 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-Q. Sun, G. Akk, J. R Huguenard, and D. A Prince Differential regulation of GABA release and neuronal excitability mediated by neuropeptide Y1 and Y2 receptors in rat thalamic neurons J. Physiol., February 15, 2001; 531(1): 81 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Cox and S. M. Sherman Glutamate Inhibits Thalamic Reticular Neurons J. Neurosci., August 1, 1999; 19(15): 6694 - 6699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. Sohal and J. R. Huguenard Long-Range Connections Synchronize Rather Than Spread Intrathalamic Oscillations: Computational Modeling and In Vitro Electrophysiology J Neurophysiol, October 1, 1998; 80(4): 1736 - 1751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. Sohal, C. L. Cox, and J. R. Huguenard Localization of CCK Receptors in Thalamic Reticular Neurons: A Modeling Study J Neurophysiol, May 1, 1998; 79(5): 2820 - 2824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Cox, J. R. Huguenard, and D. A. Prince Nucleus reticularis neurons mediate diverse inhibitory effects in thalamus PNAS, August 5, 1997; 94(16): 8854 - 8859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Miller, A. Hoffer, K. R. Svoboda, and C. R. Lupica Cholecystokinin Increases GABA Release by Inhibiting a Resting K+ Conductance in Hippocampal Interneurons J. Neurosci., July 1, 1997; 17(13): 4994 - 5003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ulrich and J. R. Huguenard Nucleus-Specific Chloride Homeostasis in Rat Thalamus J. Neurosci., April 1, 1997; 17(7): 2348 - 2354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Cox, J. R. Huguenard, and D. A. Prince Peptidergic Modulation of Intrathalamic Circuit Activity In Vitro: Actions of Cholecystokinin J. Neurosci., January 1, 1997; 17(1): 70 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Sanchez-Vives, T. Bal, U. Kim, M. von Krosigk, and D. A. McCormick Are the Interlaminar Zones of the Ferret Dorsal Lateral Geniculate Nucleus Actually Part of the Perigeniculate Nucleus? J. Neurosci., October 1, 1996; 16(19): 5923 - 5941. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
