| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Neurophysiology, Vol 75, Issue 4 1573-1588, Copyright © 1996 by APS
ARTICLES |
P. A. Salin and D. A. Prince
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, California 94305-5300, USA.
1. Spontaneous inhibitory synaptic currents (sIPSCs) were studied with whole cell voltage-clamp recordings from 131 pyramidal cells in adult rat somatosensory cortical slices. Neurons were intracellulary labeled with biocytin and classified as supragranular (SG, layers 2-3), layer IV (IV), or infragranular (IG, layer V) on the basis of the laminar localization of their somata. Somatic areas were similar for SG, IV, and IG neurons. All identified pyramidal cells generated high-frequency gamma-aminobutyric acid (GABAA) receptor-mediated synaptic events. 2. Bath application of bicuculline blocked the sIPSCs and resulted in a decrease of approximately 0.5 nS in resting conductance and an inward shift in baseline current. 3. sIPSC frequency was significantly lower in SG versus IG or IV neurons, and this difference was accounted for by the occurrence of a higher percentage of bursts of sIPSCs in the IG and IV neurons. 4. Bath application of the alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic (AMPA) receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) decreased the frequency of sIPSCs by 13-21%. By contrast, application of the N-methyl-D-aspartate (NMDA) receptor antagonist D-2-amino-5-phosphonovaleric acid (D-AP5) generally had no effect on spontaneous IPSC frequency, suggesting that AMPA rather than NMDA receptor activation contributed to resting discharge of inhibitory interneurons. 5. Addition of tetrodotoxin (TTX) to the perfusion medium reduced the spontaneous IPSC frequency by approximately 30-55%. The miniature IPSCs (mIPSCs) seen in TTX-containing solutions had a frequency of approximately 10 Hz and an average conductance of 0.42-0.48 nS. 6. The kinetic properties of mIPSCs generated in pyramidal cells of different layers were the same, with the rise times of approximately 0.9 ms and decay time constants of approximately 8 ms at a holding potential of 0 mV. The decay phase of mIPSCs was generally fitted by one exponential and displayed a voltage dependence with an e-fold increase in decay time constant for a every 198-mV depolarization. 7. These results show that there is ongoing spontaneous release of GABA in neocortical slices that gives rise to high-frequency impulse-related and non-impulse-related postsynaptic inhibitory currents. Activation of AMPA receptors on inhibitory interneurons accounts for only a small proportion of the GABAA receptor-mediated events. Judging from the distribution of mIPSC frequencies in neurons of different laminae, there is a relatively uniform distribution of inhibitory synapses throughout the cortex. Tonic activation of GABAA receptors on neocortical pyramidal neurons generates an increase in resting membrane conductance that may play an important role in vivo by preventing the development of hyperexcitability, modulating excitatory synaptic events, and controlling the rate and patterns of spike discharge.
This article has been cited by other articles:
|
|
 |
|
  C. R. Stoelzel, Y. Bereshpolova, A. G. Gusev, and H. A. Swadlow The Impact of an LGNd Impulse on the Awake Visual Cortex: Synaptic Dynamics and the Sustained/Transient Distinction J. Neurosci., May 7, 2008; 28(19): 5018 - 5028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Vierling-Claassen, P. Siekmeier, S. Stufflebeam, and N. Kopell Modeling GABA Alterations in Schizophrenia: A Link Between Impaired Inhibition and Altered Gamma and Beta Range Auditory Entrainment J Neurophysiol, May 1, 2008; 99(5): 2656 - 2671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Larsson, F. Vera Constan, N. Sebastian-Galles, and G. Deco Lexical plasticity in early bilinguals does not alter phoneme categories: I. Neurodynamical modeling. J. Cogn. Neurosci., January 1, 2008; 20(1): 76 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Rajasekaran, J. Kapur, and E. H. Bertram Alterations in GABAA Receptor Mediated Inhibition in Adjacent Dorsal Midline Thalamic Nuclei in a Rat Model of Chronic Limbic Epilepsy J Neurophysiol, November 1, 2007; 98(5): 2501 - 2508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yamada, T. Furukawa, S. Ueno, S. Yamamoto, and A. Fukuda Molecular Basis for the GABAA Receptor-Mediated Tonic Inhibition in Rat Somatosensory Cortex Cereb Cortex, August 1, 2007; 17(8): 1782 - 1787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Butovas, S. G. Hormuzdi, H. Monyer, and C. Schwarz Effects of Electrically Coupled Inhibitory Networks on Local Neuronal Responses to Intracortical Microstimulation J Neurophysiol, September 1, 2006; 96(3): 1227 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Hardie and R. A. Pearce Active and Passive Membrane Properties and Intrinsic Kinetics Shape Synaptic Inhibition in Hippocampal CA1 Pyramidal Neurons. J. Neurosci., August 15, 2006; 26(33): 8559 - 8569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Park, S. Skalska, and J. E. Stern Characterization of a Novel Tonic {gamma}-Aminobutyric AcidA Receptor-Mediated Inhibition in Magnocellular Neurosecretory Neurons and Its Modulation by Glia Endocrinology, August 1, 2006; 147(8): 3746 - 3760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tateno and H.P.C. Robinson Rate Coding and Spike-Time Variability in Cortical Neurons With Two Types of Threshold Dynamics J Neurophysiol, April 1, 2006; 95(4): 2650 - 2663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Kotaleski, D. Plenz, and K. T. Blackwell Using Potassium Currents to Solve Signal-to-Noise Problems in Inhibitory Feedforward Networks of the Striatum J Neurophysiol, January 1, 2006; 95(1): 331 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Scimemi, A. Semyanov, G. Sperk, D. M. Kullmann, and M. C. Walker Multiple and Plastic Receptors Mediate Tonic GABAA Receptor Currents in the Hippocampus J. Neurosci., October 26, 2005; 25(43): 10016 - 10024. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Keros and J. J. Hablitz Subtype-Specific GABA Transporter Antagonists Synergistically Modulate Phasic and Tonic GABAA Conductances in Rat Neocortex J Neurophysiol, September 1, 2005; 94(3): 2073 - 2085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Deco and E. T. Rolls Neurodynamics of Biased Competition and Cooperation for Attention: A Model With Spiking Neurons J Neurophysiol, July 1, 2005; 94(1): 295 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Hickmott Changes in Intrinsic Properties of Pyramidal Neurons in Adult Rat S1 During Cortical Reorganization J Neurophysiol, July 1, 2005; 94(1): 501 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Traub, D. Contreras, M. O. Cunningham, H. Murray, F. E. N. LeBeau, A. Roopun, A. Bibbig, W. B. Wilent, M. J. Higley, and M. A. Whittington Single-Column Thalamocortical Network Model Exhibiting Gamma Oscillations, Sleep Spindles, and Epileptogenic Bursts J Neurophysiol, April 1, 2005; 93(4): 2194 - 2232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Deco and E. T. Rolls Sequential Memory: A Putative Neural and Synaptic Dynamical Mechanism J. Cogn. Neurosci., February 1, 2005; 17(2): 294 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Jacobs and D. A. Prince Excitatory and Inhibitory Postsynaptic Currents in a Rat Model of Epileptogenic Microgyria J Neurophysiol, February 1, 2005; 93(2): 687 - 696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Sergeeva, N. Andreeva, M. Garret, A. Scherer, and H. L. Haas Pharmacological Properties of GABAA Receptors in Rat Hypothalamic Neurons Expressing the {epsilon}-Subunit J. Neurosci., January 5, 2005; 25(1): 88 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Deco and E. T. Rolls Synaptic and Spiking Dynamics underlying Reward Reversal in the Orbitofrontal Cortex Cereb Cortex, January 1, 2005; 15(1): 15 - 30. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. G. Jones and G. L. Woodhall Background synaptic activity in rat entorhinal cortical neurones: differential control of transmitter release by presynaptic receptors J. Physiol., January 1, 2005; 562(1): 107 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liang, E. Cagetti, R. W. Olsen, and I. Spigelman Altered Pharmacology of Synaptic and Extrasynaptic GABAA Receptors on CA1 Hippocampal Neurons Is Consistent with Subunit Changes in a Model of Alcohol Withdrawal and Dependence J. Pharmacol. Exp. Ther., September 1, 2004; 310(3): 1234 - 1245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Latham and S. Nirenberg Computing and Stability in Cortical Networks Neural Comput., July 1, 2004; 16(7): 1385 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Tseng and P. O'Donnell Dopamine-Glutamate Interactions Controlling Prefrontal Cortical Pyramidal Cell Excitability Involve Multiple Signaling Mechanisms J. Neurosci., June 2, 2004; 24(22): 5131 - 5139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Deco, E. T. Rolls, and B. Horwitz "What" and "Where" in Visual Working Memory: A Computational Neurodynamical Perspective for Integrating fMRI and Single-Neuron Data J. Cogn. Neurosci., May 1, 2004; 16(4): 683 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Grande, G. A. Kinney, G. L. Miracle, and W. J. Spain Dynamic Influences on Coincidence Detection in Neocortical Pyramidal Neurons J. Neurosci., February 25, 2004; 24(8): 1839 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Blackwell, U. Czubayko, and D. Plenz Quantitative Estimate of Synaptic Inputs to Striatal Neurons during Up and Down States In Vitro J. Neurosci., October 8, 2003; 23(27): 9123 - 9132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Brunel and X.-J. Wang What Determines the Frequency of Fast Network Oscillations With Irregular Neural Discharges? I. Synaptic Dynamics and Excitation-Inhibition Balance J Neurophysiol, July 1, 2003; 90(1): 415 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Bandrowski, J. R. Huguenard, and D. A. Prince Baseline Glutamate Levels Affect Group I and II mGluRs in Layer V Pyramidal Neurons of Rat Sensorimotor Cortex J Neurophysiol, March 1, 2003; 89(3): 1308 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Prescott and Y. De Koninck Gain control of firing rate by shunting inhibition: Roles of synaptic noise and dendritic saturation PNAS, February 18, 2003; 100(4): 2076 - 2081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. DeFazio, S. Heger, S. R. Ojeda, and S. M. Moenter Activation of A-Type {gamma}-Aminobutyric Acid Receptors Excites Gonadotropin-Releasing Hormone Neurons Mol. Endocrinol., December 1, 2002; 16(12): 2872 - 2891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Morales, S.-Y. Choi, and A. Kirkwood Dark Rearing Alters the Development of GABAergic Transmission in Visual Cortex J. Neurosci., September 15, 2002; 22(18): 8084 - 8090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fourcaud and N. Brunel Dynamics of the Firing Probability of Noisy Integrate-and-Fire Neurons Neural Comput., September 1, 2002; 14(9): 2057 - 2110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Calcagnotto, M. F. Paredes, and S. C. Baraban Heterotopic Neurons with Altered Inhibitory Synaptic Function in an Animal Model of Malformation-Associated Epilepsy J. Neurosci., September 1, 2002; 22(17): 7596 - 7605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xiang, J. R. Huguenard, and D. A. Prince Synaptic Inhibition of Pyramidal Cells Evoked by Different Interneuronal Subtypes in Layer V of Rat Visual Cortex J Neurophysiol, August 1, 2002; 88(2): 740 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li and D. A. Prince Synaptic Activity in Chronically Injured, Epileptogenic Sensory-Motor Neocortex J Neurophysiol, July 1, 2002; 88(1): 2 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Li, S. Clark, D. V. Lewis, and W. A. Wilson NMDA Receptor Antagonists Disinhibit Rat Posterior Cingulate and Retrosplenial Cortices: A Potential Mechanism of Neurotoxicity J. Neurosci., April 15, 2002; 22(8): 3070 - 3080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. O'Brien and A. J. Berger The Nonuniform Distribution of the GABAA Receptor alpha 1 Subunit Influences Inhibitory Synaptic Transmission to Motoneurons within a Motor Nucleus J. Neurosci., November 1, 2001; 21(21): 8482 - 8494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Fleidervish, C. Gebhardt, N. Astman, M. J. Gutnick, and U. Heinemann Enhanced Spontaneous Transmitter Release Is the Earliest Consequence of Neocortical Hypoxia That Can Explain the Disruption of Normal Circuit Function J. Neurosci., July 1, 2001; 21(13): 4600 - 4608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chub and M. J. O'Donovan Post-Episode Depression of GABAergic Transmission in Spinal Neurons of the Chick Embryo J Neurophysiol, May 1, 2001; 85(5): 2166 - 2176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bai, G. Zhu, P. Pennefather, M. F. Jackson, J. F. MacDonald, and B. A. Orser Distinct Functional and Pharmacological Properties of Tonic and Quantal Inhibitory Postsynaptic Currents Mediated by {gamma}-Aminobutyric AcidA Receptors in Hippocampal Neurons Mol. Pharmacol., April 1, 2001; 59(4): 814 - 824. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Gabel and J. J. LoTurco Electrophysiological and Morphological Characterization of Neurons Within Neocortical Ectopias J Neurophysiol, February 1, 2001; 85(2): 495 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Anchisi, B. Scelfo, and F. Tempia Postsynaptic Currents in Deep Cerebellar Nuclei J Neurophysiol, January 1, 2001; 85(1): 323 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Timofeev, F. Grenier, M. Bazhenov, T.J. Sejnowski, and M. Steriade Origin of Slow Cortical Oscillations in Deafferented Cortical Slabs Cereb Cortex, December 1, 2000; 10(12): 1185 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. Wong, G. Marchese, M. A. Casu, A. Ribeiro-da-Silva, A. C. Cuello, and Y. De Koninck Loss of Presynaptic and Postsynaptic Structures Is Accompanied by Compensatory Increase in Action Potential-Dependent Synaptic Input to Layer V Neocortical Pyramidal Neurons in Aged Rats J. Neurosci., November 15, 2000; 20(22): 8596 - 8606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Komatsu and Y. Yoshimura Activity-Dependent Maintenance of Long-Term Potentiation at Visual Cortical Inhibitory Synapses J. Neurosci., October 15, 2000; 20(20): 7539 - 7546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Compte, N. Brunel, P. S. Goldman-Rakic, and X.-J. Wang Synaptic Mechanisms and Network Dynamics Underlying Spatial Working Memory in a Cortical Network Model Cereb Cortex, September 1, 2000; 10(9): 910 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Harsch and H. P. C. Robinson Postsynaptic Variability of Firing in Rat Cortical Neurons: The Roles of Input Synchronization and Synaptic NMDA Receptor Conductance J. Neurosci., August 15, 2000; 20(16): 6181 - 6192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Bush, D. A. Prince, and K. D. Miller Increased Pyramidal Excitability and NMDA Conductance Can Explain Posttraumatic Epileptogenesis Without Disinhibition: A Model J Neurophysiol, October 1, 1999; 82(4): 1748 - 1758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. F. Ling and L. S. Benardo Restrictions on Inhibitory Circuits Contribute to Limited Recruitment of Fast Inhibition in Rat Neocortical Pyramidal Cells J Neurophysiol, October 1, 1999; 82(4): 1793 - 1807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. F. Owens, X. Liu, and A. R. Kriegstein Changing Properties of GABAA Receptor-Mediated Signaling During Early Neocortical Development J Neurophysiol, August 1, 1999; 82(2): 570 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Destexhe and D. Pare Impact of Network Activity on the Integrative Properties of Neocortical Pyramidal Neurons In Vivo J Neurophysiol, April 1, 1999; 81(4): 1531 - 1547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Raman and B. P. Bean Ionic Currents Underlying Spontaneous Action Potentials in Isolated Cerebellar Purkinje Neurons J. Neurosci., March 1, 1999; 19(5): 1663 - 1674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Perrais and N. Ropert Effect of Zolpidem on Miniature IPSCs and Occupancy of Postsynaptic GABAA Receptors in Central Synapses J. Neurosci., January 15, 1999; 19(2): 578 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Defazio and J. J. Hablitz Zinc and Zolpidem Modulate mIPSCs in Rat Neocortical Pyramidal Neurons J Neurophysiol, October 1, 1998; 80(4): 1670 - 1677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-K. Song, H.-W. Suh, S.-O. Huh, J.-S. Jung, B.-M. Ihn, I.-G. Choi, and Y.-H. Kim Central GABAA and GABAB Receptor Modulation of Basal and Stress-Induced Plasma Interleukin-6 Levels in Mice J. Pharmacol. Exp. Ther., October 1, 1998; 287(1): 144 - 149. [Abstract] [Full Text]
|
|
|
|
|
|
N. Tian, T. N. Hwang, and D. R. Copenhagen Analysis of Excitatory and Inhibitory Spontaneous Synaptic Activity in Mouse Retinal Ganglion Cells J Neurophysiol, September 1, 1998; 80(3): 1327 - 1340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kawaguchi and T. Shindou Noradrenergic Excitation and Inhibition of GABAergic Cell Types in Rat Frontal Cortex J. Neurosci., September 1, 1998; 18(17): 6963 - 6976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Golomb Models of Neuronal Transient Synchrony During Propagation of Activity Through Neocortical Circuitry J Neurophysiol, January 1, 1998; 79(1): 1 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-M. Zhou and J. J. Hablitz Metabotropic Glutamate Receptor Enhancement of Spontaneous IPSCs in Neocortical Interneurons J Neurophysiol, November 1, 1997; 78(5): 2287 - 2295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hajos and I. Mody Synaptic Communication among Hippocampal Interneurons: Properties of Spontaneous IPSCs in Morphologically Identified Cells J. Neurosci., November 1, 1997; 17(21): 8427 - 8442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Galarreta and S. Hestrin Properties of GABAA Receptors Underlying Inhibitory Synaptic Currents in Neocortical Pyramidal Neurons J. Neurosci., October 1, 1997; 17(19): 7220 - 7227. [Abstract] [Full Text] [PDF]
|
|
|
