Journal of Neurophysiology, Vol 76, Issue 1 47-58, Copyright © 1996 by APS

ARTICLES

Responses of gustatory cells in the nucleus of the solitary tract of the hamster after NaCl or amiloride adaptation

D. V. Smith, H. Liu and M. B. Vogt
Department of Anatomy, University of Maryland School of Medicine, Baltimore 21201-1559, USA.

1. The responses of single nucleus of the solitary tract (NST) neurons in the hamster were recorded to an array of Na+ and non-Na+ stimuli under each of three adaptation conditions: distilled H2O, 0.032 M NaCl, and 10 microM amiloride. Each adapting solution flowed for 60 s before delivery of one of seven test stimuli: 0.032 M NaCl, NaNO3, and Na-gluconate, 0.1 M KCl and sucrose, 1 mM HCl, and 3 mM quinine hydrochloride (QHCl). Stimuli were dissolved in distilled H2O (H2O and NaCl adaptation conditions) or 10 microM amiloride (amiloride adaptation condition). 2. Both amiloride treatment and NaCl adaptation reduced responses to the Na+ stimuli. The effects of NaCl adaptation were generally greater than those of amiloride, and the responses to the Na+ salts were reduced by NaCl adaptation in every cell that responded to NaCl, regardless of its best-stimulus classification. Amiloride treatment suppressed the responses to Na+ salts with larger anions (NaNO3 and Na-gluconate) more than the response to NaCl. 3. Unlike amiloride treatment, NaCl adaptation also reduced responses to several non-Na+ stimuli (KCl, HCl, and QHCl). This effect occurred primarily in the NaCl-best neurons that were most highly responsive to NaCl and that showed a postexcitatory suppression after NaCl. This suppression has been observed in recordings from the chorda tympani nerve in both rats and hamsters and in taste receptor cell responses recorded in situ in the rat. If it is a receptor phenomenon, these data would imply that some NaCl-sensitive receptor cells are also responsive to these non-Na+ electrolytes. 4. The effects of amiloride on the responses to Na+ stimuli were not limited to NaCl-best neurons, but occurred in sucrose-best cells as well. These results suggest that the sucrose-best cells in the NST receive converging input from sucrose- and NaCl-best chorda tympani fibers, because there is little Na+ sensitivity in the peripheral sucrose-best fibers and the amiloride sensitivity is restricted to NaCl-best chorda tympani fibers. The responses to NaCl in the few HCl- and QHCl-best NST neurons were not affected by amiloride. 5. Rinsing the tongue with amiloride for 60 s resulted in a reduction in the baseline response rate of NST cells. This effect occurred primarily in NaCl- and sucrose-best NST neurons and implies that much of the spontaneous activity in these brain stem cells arises from amiloride-sensitive channel activity in the peripheral receptor cells. 6. The results of human psychophysical studies show very different effects of NaCl adaptation and amiloride treatment. Adaptation to NaCl produces a robust and specific reduction in the saltiness of all salts. The present results show that NaCl adaptation reduces the responses of all cells sensitive to NaCl. Treatment of the human tongue with amiloride produces a proportionately smaller reduction in the response to NaCl than it does in rodents, and it appears to have no effect on saltiness. Rather, amiloride has been shown to specifically reduce the sour side taste of NaCl, Nagluconate, and LiCl. Therefore conclusions about the effects of amiloride on taste quality based on rodent electrophysiology are questionable.

This article has been cited by other articles:

				C. H. Lemon and D. V. Smith Neural Representation of Bitter Taste in the Nucleus of the Solitary Tract J Neurophysiol, December 1, 2005; 94(6): 3719 - 3729. [Abstract] [Full Text] [PDF]

				J. V. Verhagen, B. K. Giza, and T. R. Scott Effect of Amiloride on Gustatory Responses in the Ventroposteromedial Nucleus of the Thalamus in Rats J Neurophysiol, January 1, 2005; 93(1): 157 - 166. [Abstract] [Full Text] [PDF]

				C. H. Lemon, T. Imoto, and D. V. Smith Differential Gurmarin Suppression of Sweet Taste Responses in Rat Solitary Nucleus Neurons J Neurophysiol, August 1, 2003; 90(2): 911 - 923. [Abstract] [Full Text] [PDF]

				S. J. St. John and D. V. Smith Neural Representation of Salts in the Rat Solitary Nucleus: Brain Stem Correlates of Taste Discrimination J Neurophysiol, August 1, 2000; 84(2): 628 - 638. [Abstract] [Full Text] [PDF]

				R. F. Lundy Jr. and R. J. Contreras Gustatory Neuron Types in Rat Geniculate Ganglion J Neurophysiol, December 1, 1999; 82(6): 2970 - 2988. [Abstract] [Full Text] [PDF]

				M. A. Barry Recovery of Functional Response in the Nucleus of the Solitary Tract After Peripheral Gustatory Nerve Crush and Regeneration J Neurophysiol, July 1, 1999; 82(1): 237 - 247. [Abstract] [Full Text] [PDF]

				J. D. Boughter Jr., S. J. St. John, and D. V. Smith Neural Representation of the Taste of NaCl and KCl in Gustatory Neurons of the Hamster Solitary Nucleus J Neurophysiol, June 1, 1999; 81(6): 2636 - 2646. [Abstract] [Full Text] [PDF]

				J. D. Boughter Jr. and D. V. Smith Amiloride Blocks Acid Responses in NaCl-Best Gustatory Neurons of the Hamster Solitary Nucleus J Neurophysiol, September 1, 1998; 80(3): 1362 - 1372. [Abstract] [Full Text] [PDF]

				R. E. Stewart, V. Lyall, G. M. Feldman, G. L. Heck, and J. A. DeSimone Acid-induced responses in hamster chorda tympani and intracellular pH tracking by taste receptor cells Am J Physiol Cell Physiol, July 1, 1998; 275(1): C227 - C238. [Abstract] [Full Text] [PDF]

				X. Zhang, W. E. Renehan, and R. Fogel Neurons in the vagal complex of the rat respond to mechanical and chemical stimulation of the GI tract Am J Physiol Gastrointest Liver Physiol, February 1, 1998; 274(2): G331 - G341. [Abstract] [Full Text] [PDF]

				C.-S. Li and D. V. Smith Glutamate Receptor Antagonists Block Gustatory Afferent Input to the Nucleus of the Solitary Tract J Neurophysiol, March 1, 1997; 77(3): 1514 - 1525. [Abstract] [Full Text] [PDF]