JN AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 76: 3559-3562, 1996;
0022-3077/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilk-Blaszczak, M. A.
Right arrow Articles by Belardetti, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilk-Blaszczak, M. A.
Right arrow Articles by Belardetti, F.

Journal of Neurophysiology, Vol 76, Issue 5 3559-3562, Copyright © 1996 by APS

ARTICLES

Three distinct G protein pathways mediate inhibition of neuronal calcium current by bradykinin

M. A. Wilk-Blaszczak, W. D. Singer and F. Belardetti
Department of Pharmacology, University of Texas, Southwestern Medical Center, Dallas 75235, USA.

1. In NG108-15 cells dialyzed with 10 mM ethylene glycolbis (beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) or bis (o-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA), bradykinin (BK) selectively inhibited the N-type calcium current. This effect of BK was blocked by an antibody directed against the G protein G13. Thus under these conditions G13 mediates the inhibition of voltage-dependent calcium current (ICa, V) by BK. In contrast, activation of K+ currents by BK is mediated by Gq/11. BK also couples to Gi2. 2. We now examine the involvement of G proteins in the inhibition of ICa, V by BK when NG108-15 cells are dialyzed with 1 mM BAPTA. Under these conditions, BK inhibited both the N- and L-type, but not the T-type, calcium currents. Intracellular application of anti-G13 antibody did not suppress the response to BK. Applications of either anti-Gq/11 antibody or pertussis toxin (PTX, to block Gi2) were similarly ineffective. Even combined application of anti-Gq/11 and -G13 antibodies, or PTX together with either antibody, did not block inhibition of ICa, V by BK. However, the combination of both antibodies with PTX blocked the response to BK in low BAPTA. In conclusion, both Gq/11 and a PTX-sensitive G protein (presumably Gi2), together with G13, are involved in the inhibition of ICa, V by BK. 3. Gq/11 inhibited only the L-type calcium current, whereas the PTX-sensitive G protein inhibited both the N- and L-type calcium currents. 4. The BAPTA dependence of the Gq/11 and PTX-sensitive inhibitions may reflect a Ca2+ requirement of the pathway(s) acting on the L current and/or a direct suppressive effect of BAPTA.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
G. N. Prado, D. F. Mierke, M. Pellegrini, L. Taylor, and P. Polgar
Motif Mutation of Bradykinin B2 Receptor Second Intracellular Loop and Proximal C Terminus Is Critical for Signal Transduction, Internalization, and Resensitization
J. Biol. Chem., December 11, 1998; 273(50): 33548 - 33555.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Wilk-Blaszczak, B. Stein, S. Xu, M. S. Barbosa, M. H. Cobb, and F. Belardetti
The Mitogen-Activated Protein Kinase p38-2 Is Necessary for the Inhibition of N-Type Calcium Current by Bradykinin
J. Neurosci., January 1, 1998; 18(1): 112 - 118.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. A. Wilk-Blaszczak, W. D. Singer, T. Quill, B. Miller, J. A. Frost, P. C. Sternweis, and F. Belardetti
The Monomeric G-Proteins Rac1 and/or Cdc42 Are Required for the Inhibition of Voltage-Dependent Calcium Current by Bradykinin
J. Neurosci., June 1, 1997; 17(11): 4094 - 4100.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online