| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Neurophysiology, Vol 76, Issue 6 3886-3894, Copyright © 1996 by APS
ARTICLES |
K. L. Perkins and R. K. Wong
Department of Pharmacology, State University of New York Health Science Center, Brooklyn 11203, USA.
1. Whole cell voltage-clamp recording with recording pipette solutions of differing ionic composition was used to determine the ionic basis of the depolarizing gamma-aminobutyric acid (GABA) response. In the presence of 4-aminopyridine and excitatory amino acid receptor blockers, giant GABA-mediated postsynaptic currents (GPSCs) were recorded from CA3 pyramidal neurons in hippocampal slices from adult guinea pigs. With the GABAB component blocked, the GPSC was composed of an initial outward current (GABAA component) that peaked at 115 ms followed by a late inward current (GABAD component) that peaked at 400-600 ms. 2. Reduction of the intracellular concentration of potassium ([K+]i)resulted in no significant change in the reversal potential of the GABAD component of the GPSC, indicating that it is not a nonspecific cation current. 3. The HCO3- permeability of the channel mediating the GABAD response was assessed by using recording pipette solutions containing three different concentrations of bicarbonate ([HCO3-], 19, 49, and 102 mM). The reversal potential of the GABAD response shifted in the depolarizing direction as the HCO3- equilibrium potential was shifted in the depolarizing direction, indicating that the channel mediating the GABAD response is permeable to HCO3-. The reversal potential of the GABAD response was more sensitive to changes in recording pipette [HCO3-] than the reversal potential of the GABAA response, indicating that the GABAD response is carried by HCO3- to a greater extent than the GABAA response. 4. The outward current-inward current sequence of the biphasic GPSC was reversed to an inward current-outward current sequence by using a high [Cl-]/low [HCO3-] recording pipette solution (40 mM Cl-/6 mM HCO3-), indicating that the GABAA component is more sensitive to changes in [Cl-]i, and the GABAD component is more sensitive to changes in [HCO3-]i. 5. These data indicate that the GABAD component of the GPSC is predominantly carried by HCO3-. While this result supports the recently propsed chloride accumulation model, the model in its present form cannot explain the inward current-outward current polarity sequence of the GPSC recorded with the high [Cl-]/low [HCO3-] intracellular solution. The data obtained using that solution reveal the need for a more expansive chloride accumulation/ depletion model or for a model utilizing two distinct ionotropic GABA channels with different anion permeability ratios to account for the biphasic nature of the GPSC.
This article has been cited by other articles:
|
|
 |
|
  E. Ducret, Y. Le Feuvre, P. Meyrand, and V. S. Fenelon Removal of GABA within Adult Modulatory Systems Alters Electrical Coupling and Allows Expression of an Embryonic-Like Network J. Neurosci., April 4, 2007; 27(14): 3626 - 3638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-Y. Fu and A. N. van den Pol GABA excitation in mouse hilar neuropeptide Y neurons J. Physiol., March 1, 2007; 579(2): 445 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Zsiros, I. Aradi, and G. Maccaferri Propagation of postsynaptic currents and potentials via gap junctions in GABAergic networks of the rat hippocampus J. Physiol., January 15, 2007; 578(2): 527 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Karst and M. Joels Corticosterone Slowly Enhances Miniature Excitatory Postsynaptic Current Amplitude in Mice CA1 Hippocampal Cells J Neurophysiol, November 1, 2005; 94(5): 3479 - 3486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Kantrowitz, N. N. Francis, A. Salah, and K. L. Perkins Synaptic Depolarizing GABA Response in Adults Is Excitatory and Proconvulsive When GABAB Receptors Are Blocked J Neurophysiol, May 1, 2005; 93(5): 2656 - 2667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fujiwara-Tsukamoto, Y. Isomura, K. Kaneda, and M. Takada Synaptic interactions between pyramidal cells and interneurone subtypes during seizure-like activity in the rat hippocampus J. Physiol., June 15, 2004; 557(3): 961 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Isomura, M. Sugimoto, Y. Fujiwara-Tsukamoto, S. Yamamoto-Muraki, J. Yamada, and A. Fukuda Synaptically Activated Cl- Accumulation Responsible for Depolarizing GABAergic Responses in Mature Hippocampal Neurons J Neurophysiol, October 1, 2003; 90(4): 2752 - 2756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. McQuiston and P. Saggau Mu-opioid Receptors Facilitate the Propagation of Excitatory Activity in Rat Hippocampal Area CA1 by Disinhibition of all Anatomical Layers J Neurophysiol, September 1, 2003; 90(3): 1936 - 1948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Perkins GABA Application to Hippocampal CA3 or CA1 Stratum Lacunosum-Moleculare Excites an Interneuron Network J Neurophysiol, March 1, 2002; 87(3): 1404 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W. Vanderklish and G. M. Edelman Dendritic spines elongate after stimulation of group 1 metabotropic glutamate receptors in cultured hippocampal neurons PNAS, January 24, 2002; (2002) 32681099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Mazer, W. E. Vinje, J. McDermott, P. H. Schiller, and J. L. Gallant Spatial frequency and orientation tuning dynamics in area V1 PNAS, January 24, 2002; (2002) 22638499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Gusev and D. L. Alkon Intracellular Correlates of Spatial Memory Acquisition in Hippocampal Slices: Long-Term Disinhibition of CA1 Pyramidal Cells J Neurophysiol, August 1, 2001; 86(2): 881 - 899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Patrylo, D. D. Spencer, and A. Williamson GABA Uptake and Heterotransport Are Impaired in the Dentate Gyrus of Epileptic Rats and Humans With Temporal Lobe Sclerosis J Neurophysiol, April 1, 2001; 85(4): 1533 - 1542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kulik, H. Nishimaru, and K. Ballanyi Role of Bicarbonate and Chloride in GABA- and Glycine-Induced Depolarization and [Ca2+]i Rise in Fetal Rat Motoneurons In Situ J. Neurosci., November 1, 2000; 20(21): 7905 - 7913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kohling, M. Vreugdenhil, E. Bracci, and J. G. R. Jefferys Ictal Epileptiform Activity Is Facilitated by Hippocampal GABAA Receptor-Mediated Oscillations J. Neurosci., September 15, 2000; 20(18): 6820 - 6829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Schweitzer, H. Wang, Z.-Q. Xiong, and J. L. Stringer pH Sensitivity of Non-Synaptic Field Bursts in the Dentate Gyrus J Neurophysiol, August 1, 2000; 84(2): 927 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Smirnov, P. Paalasmaa, M. Uusisaari, J. Voipio, and K. Kaila Pharmacological Isolation of the Synaptic and Nonsynaptic Components of the GABA-Mediated Biphasic Response in Rat CA1 Hippocampal Pyramidal Cells J. Neurosci., November 1, 1999; 19(21): 9252 - 9260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Wang, K. M. Raley-Susman, J. Wang, G. Chambers, J. E. Cottrell, I. S. Kass, and D. J. Cole Thiopental Attenuates Hypoxic Changes of Electrophysiology, Biochemistry, and Morphology in Rat Hippocampal Slice CA1 Pyramidal Cells • Editorial Comment Stroke, November 1, 1999; 30(11): 2400 - 2407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Perkins Cl- Accumulation Does Not Account for the Depolarizing Phase of the Synaptic GABA Response in Hippocampal Pyramidal Cells J Neurophysiol, August 1, 1999; 82(2): 768 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Jarolimek, A. Lewen, and U. Misgeld A Furosemide-Sensitive K+-Cl- Cotransporter Counteracts Intracellular Cl- Accumulation and Depletion in Cultured Rat Midbrain Neurons J. Neurosci., June 15, 1999; 19(12): 4695 - 4704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Jackson, B. Esplin, and R. Capek Inhibitory Nature of Tiagabine-Augmented GABAA Receptor-Mediated Depolarizing Responses in Hippocampal Pyramidal Cells J Neurophysiol, March 1, 1999; 81(3): 1192 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. B. Liu, G.-L. Ye, X.-S. Liu, J. F. Pasternak, and B. L. Trommer GABAA Currents in Immature Dentate Gyrus Granule Cells J Neurophysiol, November 1, 1998; 80(5): 2255 - 2267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Forti and H. B. Michelson Synaptic Connectivity of Distinct Hilar Interneuron Subpopulations J Neurophysiol, June 1, 1998; 79(6): 3229 - 3237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Barnard, P. Skolnick, R. W. Olsen, H. Mohler, W. Sieghart, G. Biggio, C. Braestrup, A. N. Bateson, and S. Z. Langer International Union of Pharmacology. XV. Subtypes of gamma -Aminobutyric AcidA Receptors: Classification on the Basis of Subunit Structure and Receptor Function Pharmacol. Rev., June 1, 1998; 50(2): 291 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fukuda, K. Muramatsu, A. Okabe, Y. Shimano, H. Hida, I. Fujimoto, and H. Nishino Changes in Intracellular Ca2+ Induced by GABAA Receptor Activation and Reduction in Cl- Gradient in Neonatal Rat Neocortex J Neurophysiol, January 1, 1998; 79(1): 439 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kaila, K. Lamsa, S. Smirnov, T. Taira, and J. Voipio Long-Lasting GABA-Mediated Depolarization Evoked by High-Frequency Stimulation in Pyramidal Neurons of Rat Hippocampal Slice Is Attributable to a Network-Driven, Bicarbonate-Dependent K+ Transient J. Neurosci., October 15, 1997; 17(20): 7662 - 7672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-Y. Chen, E.-h. Ling, J. M. Horowitz, and A. C. Bonham Synaptic transmission in nucleus tractus solitarius is depressed by Group II and III but not Group I presynaptic metabotropic glutamate receptors in rats J. Physiol., February 1, 2002; 538(3): 773 - 786. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
