The Journal of Neurophysiology Vol. 77 No. 2 February 1997, pp. 717-730
Copyright ©1997 The American Physiological Society

Intrinsic NMDA-Induced Oscillations in Motoneurons of an Adult Vertebrate Spinal Cord Are Masked by Inhibition

Mengia-Seraina Rioult-Pedotti

Department of Biology, Yale University, New Haven, Connecticut 06520-8103

Rioult-Pedotti, Mengia-Seraina. Intrinsic NMDA induced oscillations in motoneurons of an adult vertebrate spinal cord are masked by inhibition. J. Neurophysiol. 77: 717-730, 1997. Low-frequency membrane potential oscillations were induced in motoneurons (MNs) of isolated hemisected frog spinal cords during N-methyl-D-aspartate (NMDA) application. Oscillations required the presence of physiological Mg²⁺ and preincubation with strychnine, whereas incubation with bicuculline or phaclofen was not effective. Oscillations were evident in intracellular recordings from single MNs and simultaneous extracellular recordings from lumbar ventral roots. In Mg²⁺-free solution, MNs exhibited irregular transient membrane potential depolarizations that were blocked by D,L-2-amino-5-phosphonopentanoic acid (APV) but not by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Generation and maintenance of membrane potential oscillations required specific NMDA receptor activation. Oscillations were antagonized by APV but not by CNQX. Strychnine preincubation was required for NMDA to induce oscillations, but was not critical in maintaining them, because oscillations persisted after removal of strychnine. Therefore oscillations are suggested to be an inherent property of the spinal neuronal circuitry. Tetrodotoxin (TTX) blocked spike activity and had a bimodal effect on membrane potential oscillations. Oscillations initially were blocked by TTX, but reappeared spontaneously after 10-40 min. This suggests that maintenance of oscillations, once evoked, does not involve MN firing. Na⁺ entry through TTX-insensitive Na⁺ channels and/or NMDA receptor channels, transmembrane Ca²⁺ flux, Ca²⁺ release from intracellular stores, and Ca²⁺ activated K⁺ channels were critical in controlling the amplitude and frequency of membrane potential oscillations. It is hypothesized that these unmasked intrinsic oscillations in adult frog spinal cord MNs may represent a premetamorphic spinal oscillator involved in tadpole swimming that becomes suppressed during metamorphosis as strychnine-sensitive inhibition becomes more pronounced.

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