JN Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 77: 1338-1348, 1997;
0022-3077/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wiley, J. W.
Right arrow Articles by Macdonald, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wiley, J. W.
Right arrow Articles by Macdonald, R. L.

The Journal of Neurophysiology Vol. 77 No. 3 March 1997, pp. 1338-1348
Copyright ©1997 The American Physiological Society

Dynorphin A-Mediated Reduction in Multiple Calcium Currents Involves a Goalpha -Subtype G Protein in Rat Primary Afferent Neurons

John W. Wiley1, 4, Hylan C. Moises2, Robert A. Gross3, and Robert L. Macdonald2, 3

1 Department of Internal Medicine, 2 Department of Physiology, and 3 Department of Neurology, The University of Michigan, Ann Arbor; and 4 Veterans Affairs Medical Center, Ann Arbor, Michigan 48105

Wiley, John W., Hylan C. Moises, Robert A. Gross, and Robert L. Macdonald. Dynorphin A-mediated reduction in multiple calcium currents involves a Goalpha -subtype G protein in rat primary afferent neurons. J. Neurophysiol. 77: 1338-1348, 1997. We examined the effect of antisera directed at specific G-protein subtype(s) on dynorphin A (Dyn A)-mediated reduction of calcium currents in rat dorsal root ganglia (DRG) neurons. Whole cell patch-clamp recordings were performed on acutely dissociated neurons. Dyn A (1 µM)-mediated decrease in calcium currents was inhibited >90% by the preferential kappa -receptor antagonist norbinaltorphimine. Dyn A (300-1,000 nM)-mediated reduction in calcium currents was examined during intracellular administration of antisera directed against specific regions of Goalpha , Gi1alpha /Gi2alpha , and Gi3alpha subunits. Intracellular dialysis with an antiserum specific for Goalpha for 20 min decreased calcium current inhibition by Dyn A (1 µM) in 13 of 15 neurons by an average of 75%. Dialysis with nonimmune serum did not affect Dyn A's action to reduce calcium currents. Intracellular dialysis with either anti-Gi1alpha /Gi2alpha or anti-Gi3alpha antisera did not affect Dyn A-induced changes in calcium currents. In the presence of the N-type calcium channel antagonist omega -conotoxin GVIA, the P-type calcium channel antagonist omega -Aga IVA, and omega -Aga MVIIC applied subsequent to the other toxins, the effect of Dyn A to reduce calcium currents was inhibited by 52, 28, and 16%, respectively. The L channel antagonist nifedipine did not affect the ability of Dyn A to inhibit calcium currents. These results suggest that in rat DRG neurons coupling of kappa -opioid receptors to multiple transient, high-threshold calcium currents involves the Goalpha subclass of G proteins.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
Y. Li and A. N. van den Pol
Differential Target-Dependent Actions of Coexpressed Inhibitory Dynorphin and Excitatory Hypocretin/Orexin Neuropeptides
J. Neurosci., December 13, 2006; 26(50): 13037 - 13047.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
I. A. Khasabova, C. Harding-Rose, D. A. Simone, and V. S. Seybold
Differential Effects of CB1 and Opioid Agonists on Two Populations of Adult Rat Dorsal Root Ganglion Neurons
J. Neurosci., February 18, 2004; 24(7): 1744 - 1753.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. de los Santos-Arteaga, S. A. Sierra-Dominguez, G. H. Fontanella, J. M. Delgado-Garcia, and A. M. Carrion
Analgesia Induced by Dietary Restriction Is Mediated by the {kappa}-Opioid System
J. Neurosci., December 3, 2003; 23(35): 11120 - 11126.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. E. Hall, J. Liu, A. A. F. Sima, and J. W. Wiley
Impaired Inhibitory G-Protein Function Contributes to Increased Calcium Currents in Rats With Diabetic Neuropathy
J Neurophysiol, August 1, 2001; 86(2): 760 - 770.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
P. L. Prather, L. Song, E. T. Piros, P. Y. Law, and T. G. Hales
delta -Opioid Receptors Are More Efficiently Coupled to Adenylyl Cyclase Than to L-Type Ca2+ Channels in Transfected Rat Pituitary Cells
J. Pharmacol. Exp. Ther., November 1, 2000; 295(2): 552 - 562.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
C. G. Acosta and H. S. Lopez
delta Opioid Receptor Modulation of Several Voltage-Dependent Ca2+ Currents in Rat Sensory Neurons
J. Neurosci., October 1, 1999; 19(19): 8337 - 8348.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. P.J. King, K. E. Hall, and R. L. Macdonald
kappa - and µ-Opioid Inhibition of N-Type Calcium Currents Is Attenuated by 4beta -Phorbol 12-Myristate 13-Acetate and Protein Kinase C in Rat Dorsal Root Ganglion Neurons
J. Pharmacol. Exp. Ther., April 1, 1999; 289(1): 312 - 320.
[Abstract] [Full Text]

Home page
 J. Neurophysiol.Home page
X. Su, R. E. Wachtel, and G. F. Gebhart
Inhibition of Calcium Currents in Rat Colon Sensory Neurons by K- But Not µ- or delta -Opioids
J Neurophysiol, December 1, 1998; 80(6): 3112 - 3119.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H. Ristic, S. Srinivasan, K. E. Hall, A. A. F. Sima, and J. W. Wiley
Serum From Diabetic BB/W Rats Enhances Calcium Currents in Primary Sensory Neurons
J Neurophysiol, September 1, 1998; 80(3): 1236 - 1244.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Kinoshita, T. Nukada, T. Asano, Y. Mori, A. Akaike, M. Satoh, and S. Kaneko
Binding of Galpha o N Terminus Is Responsible for the Voltage-resistant Inhibition of alpha 1A (P/Q-type, Cav2.1) Ca2+ Channels
J. Biol. Chem., July 27, 2001; 276(31): 28731 - 28738.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online