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J Neurophysiol 77: 1418-1424, 1997;
0022-3077/97 $5.00
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The Journal of Neurophysiology Vol. 77 No. 3 March 1997, pp. 1418-1424
Copyright ©1997 The American Physiological Society

Modulation of High-Voltage Activated Ca2+ Channels in the Rat Periaqueductal Gray Neurons by µ-Type Opioid Agonist

Chang-Ju Kim, Jeong-Seop Rhee, and Norio Akaike

Department of Physiology, Kyung Hee University College of Medicine, Seoul, Korea; and Department of Physiology, Kyushu University Faculty of Medicine, Fukuoka 812-82, Japan

Kim, Chang-Ju, Jeong-Seop Rhee, and Norio Akaike. Modulation of high-voltage activated Ca2+ channels in the rat periaqueductal gray neurons by µ-type opioid agonist. J. Neurophysiol. 77: 1418-1424, 1997. The effect of µ-type opioid receptor agonist, D-Ala2,N-MePhe4,Gly5-ol-enkephalin (DAMGO), on high-voltage-activated (HVA) Ca2+ channels in the dissociated rat periaqueductal gray (PAG) neurons was investigated by the use of nystatin-perforated patch recording mode under voltage-clamp condition. Among 118 PAG neurons tested, the HVA Ca2+ channels of 38 neurons (32%) were inhibited by DAMGO (DAMGO-sensitive cells), and the other 80 neurons (68%) were not affected by DAMGO (DAMGO-insensitive cells). The N-, P-, L-, Q-, and R-type Ca2+ channel components in DAMGO-insensitive cells shared 26.9, 37.1, 22.3, 7.9, and 5.8%, respectively, of the total Ca2+ channel current. The channel components of DAMGO-sensitive cells were 45.6, 25.7, 21.7, 4.6, and 2.4%, respectively. The HVA Ca2+ current of DAMGO-sensitive neurons was inhibited by DAMGO in a concentration-, time-, and voltage-dependent manner. Application of omega -conotoxin-GVIA occluded the inhibitory effect of DAMGO ~70%. So, HVA Ca2+ channels inhibited by DAMGO were mainly the N-type Ca2+ channels. The inhibitory effect of DAMGO on HVA Ca2+ channels was prevented almost completely by the pretreatment of pertussis toxin (PTX) for 8-10 h, suggesting that DAMGO modulation on N-type Ca2+ channels in rat PAG neurons is mediated by PTX-sensitive G proteins. These results indicate that µ-type opioid receptor modulates N-type HVA Ca2+ channels via PTX-sensitive G proteins in PAG neurons of rats.




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