JN Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 77: 3396-3400, 1997;
0022-3077/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (85)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boudaba, C.
Right arrow Articles by Tasker, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boudaba, C.
Right arrow Articles by Tasker, J. G.

The Journal of Neurophysiology Vol. 77 No. 6 June 1997, pp. 3396-3400
Copyright ©1997 The American Physiological Society

RAPID COMMUNICATION


Physiological Evidence for Local Excitatory Synaptic Circuits in the Rat Hypothalamus

Cherif Boudaba1, Laura A. Schrader2, and Jeffrey G. Tasker1, 2

1 Department of Cell and Molecular Biology and 2 Neuroscience Graduate Program, Tulane University, New Orleans, Louisiana 70118

Boudaba, Cherif, Laura A. Schrader, and Jeffrey G. Tasker. Physiological evidence for local excitatory synaptic circuits in the rat hypothalamus. J. Neurophysiol. 77: 3396-3400, 1997. We conducted whole cell voltage-clamp and current-clamp recordings in slices of rat hypothalamus to test for local excitatory synaptic circuits. Local excitatory inputs to neurons of the paraventricular nucleus (PVN) and supraoptic nucleus (SON) were studied with the use of electrical and chemical stimulation. Extracellular electrical stimulation provided indirect evidence of local excitatory circuits. Single stimuli evoked multiple excitatory postsynaptic potentials (EPSPs) or excitatory postsynaptic currents (EPSCs) in some PVN and SON cells, invoking polysynaptic excitatory inputs. Repetitive stimulation (10-20 Hz, 2-10 s) elicited long afterdischarges of EPSPs/EPSCs, suggesting a potentiation of upstream synapses in a polysynaptic circuit. Bath application of metabotropic glutamate receptor agonists provided more conclusive evidence for local excitatory circuits. Metabotropic receptor activation caused an increase in the frequency of EPSPs/EPSCs that was blocked by tetrodotoxin, suggesting that it was mediated by activation of local presynaptic excitatory neurons. The local excitatory inputs to SON and PVN neurons were mediated by glutamate release, because the EPSPs/EPSCs elicited with electrical stimulation and metabotropic receptor activation were blocked by ionotropic glutamate receptor antagonists. Finally, glutamate microstimulation furnished the most direct demonstration of local excitatory synaptic circuits. Glutamate microstimulation of perinuclear sites elicited an increase in the frequency of EPSPs/EPSCs in 13% of the PVN and SON neurons tested. Two sites provided most of the local excitatory synaptic inputs to PVN neurons, the dorsomedial hypothalamus and the perifornical region. These experiments provide converging physiological evidence for local excitatory synaptic inputs to hypothalamic neurons, inputs that may play a role in pulsatile hormone release.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
Q.-H. Chen and G. M. Toney
Excitability of paraventricular nucleus neurones that project to the rostral ventrolateral medulla is regulated by small-conductance Ca2+-activated K+ channels
J. Physiol., September 1, 2009; 587(17): 4235 - 4247.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Al-Noori, N. M. Sanders, G. J. Taborsky Jr., C. W. Wilkinson, A. Zavosh, C. West, C. M. Sanders, and D. P. Figlewicz
Recurrent hypoglycemia alters hypothalamic expression of the regulatory proteins FosB and synaptophysin
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1446 - R1454.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. E. Quinlan, C. O. Alberto, and M. Hirasawa
Short-term potentiation of mEPSCs requires N-, P/Q- and L-type Ca2+ channels and mitochondria in the supraoptic nucleus
J. Physiol., July 1, 2008; 586(13): 3147 - 3161.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D.-P. Li, Q. Yang, H.-M. Pan, and H.-L. Pan
Pre- and postsynaptic plasticity underlying augmented glutamatergic inputs to hypothalamic presympathetic neurons in spontaneously hypertensive rats
J. Physiol., March 15, 2008; 586(6): 1637 - 1647.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. J. Iremonger and J. S. Bains
Integration of Asynchronously Released Quanta Prolongs the Postsynaptic Spike Window
J. Neurosci., June 20, 2007; 27(25): 6684 - 6691.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
D.-P. Li and H.-L. Pan
Glutamatergic Inputs in the Hypothalamic Paraventricular Nucleus Maintain Sympathetic Vasomotor Tone in Hypertension
Hypertension, April 1, 2007; 49(4): 916 - 925.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Boudaba and J. G. Tasker
Internuclear coupling of hypothalamic magnocellular nuclei by glutamate synaptic circuits
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R102 - R111.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K.-Z. Shen and S. W. Johnson
Subthalamic stimulation evokes complex EPSCs in the rat substantia nigra pars reticulata in vitro
J. Physiol., June 15, 2006; 573(3): 697 - 709.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. T. Theodosis, A. Trailin, and D. A. Poulain
Remodeling of astrocytes, a prerequisite for synapse turnover in the adult brain? Insights from the oxytocin system of the hypothalamus
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1175 - R1182.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Di, C. Boudaba, I. R. Popescu, F.-J. Weng, C. Harris, V. L. Marcheselli, N. G. Bazan, and J. G. Tasker
Activity-dependent release and actions of endocannabinoids in the rat hypothalamic supraoptic nucleus
J. Physiol., December 15, 2005; 569(3): 751 - 760.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. K. Mueller, S. Di, C. M. Paden, and J. P. Herman
Activity-Dependent Modulation of Neurotransmitter Innervation to Vasopressin Neurons of the Supraoptic Nucleus
Endocrinology, January 1, 2005; 146(1): 348 - 354.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. C. Gore
Gonadotropin-Releasing Hormone Neurons: Multiple Inputs, Multiple Outputs
Endocrinology, September 1, 2004; 145(9): 4016 - 4017.
[Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
D.-P. Li, S.-R. Chen, and H.-L. Pan
VR1 Receptor Activation Induces Glutamate Release and Postsynaptic Firing in the Paraventricular Nucleus
J Neurophysiol, September 1, 2004; 92(3): 1807 - 1816.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Shirakawa, M. Mitome, T. Kikuiri, W. Nakamura, S. Oshima, T. Hasegawa, M. Shindoh, and H. Oguchi
Immobilization Induces Acute Nitric Oxide Production in the Rat Hypothalamus: A Role of Ionotropic Glutamate Receptors in the Paraventricular Nucleus
Endocrinology, August 1, 2004; 145(8): 3603 - 3607.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. J. Latchford and A. V. Ferguson
ANG II-induced excitation of paraventricular nucleus magnocellular neurons: a role for glutamate interneurons
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2004; 286(5): R894 - R902.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. B. Evans, C. W. Wilkinson, P. Gronbeck, J. L. Bennett, A. Zavosh, G. J. Taborsky Jr, and D. P. Figlewicz
Inactivation of the DMH selectively inhibits the ACTH and corticosterone responses to hypoglycemia
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2004; 286(1): R123 - R128.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. Badoer, C.-W. Ng, and R. De Matteo
Glutamatergic input in the PVN is important in renal nerve response to elevations in osmolality
Am J Physiol Renal Physiol, October 1, 2003; 285(4): F640 - F650.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Q. H. Chen, J. R. Haywood, and G. M. Toney
Sympathoexcitation by PVN-Injected Bicuculline Requires Activation of Excitatory Amino Acid Receptors
Hypertension, October 1, 2003; 42(4): 725 - 731.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
W. Lin, K. McKinney, L. Liu, S. Lakhlani, and L. Jennes
Distribution of Vesicular Glutamate Transporter-2 Messenger Ribonucleic Acid and Protein in the Septum-Hypothalamus of the Rat
Endocrinology, February 1, 2003; 144(2): 662 - 670.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. J Follwell and A. V Ferguson
Cellular mechanisms of orexin actions on paraventricular nucleus neurones in rat hypothalamus
J. Physiol., December 15, 2002; 545(3): 855 - 867.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
D. R. Ziegler and J. P. Herman
Neurocircuitry of Stress Integration: Anatomical Pathways Regulating the Hypothalamo-Pituitary-Adrenocortical Axis of the Rat
Integr. Comp. Biol., July 1, 2002; 42(3): 541 - 551.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. C. Kuehl-Kovarik, W. A. Pouliot, G. L. Halterman, R. J. Handa, F. E. Dudek, and K. M. Partin
Episodic Bursting Activity and Response to Excitatory Amino Acids in Acutely Dissociated Gonadotropin-Releasing Hormone Neurons Genetically Targeted with Green Fluorescent Protein
J. Neurosci., March 15, 2002; 22(6): 2313 - 2322.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. J. Morsette, H. Sidorowicz, and C. D. Sladek
Role of metabotropic glutamate receptors in vasopressin and oxytocin release
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2001; 281(2): R452 - R458.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. M. Morin, E. H. Stotz-Potter, and J. A. Dimicco
Injection of muscimol in dorsomedial hypothalamus and stress-induced Fos expression in paraventricular nucleus
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2001; 280(5): R1276 - R1284.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. W. Bailey and J. A. Dimicco
Chemical stimulation of the dorsomedial hypothalamus elevates plasma ACTH in conscious rats
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R8 - R15.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. B. Kombian, M. Hirasawa, D. Mouginot, X. Chen, and Q. J. Pittman
Short-Term Potentiation of Miniature Excitatory Synaptic Currents Causes Excitation of Supraoptic Neurons
J Neurophysiol, May 1, 2000; 83(5): 2542 - 2553.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. S. Daftary, C. Boudaba, K. Szabo, and J. G. Tasker
Noradrenergic Excitation of Magnocellular Neurons in the Rat Hypothalamic Paraventricular Nucleus via Intranuclear Glutamatergic Circuits
J. Neurosci., December 15, 1998; 18(24): 10619 - 10628.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Peyron, D. K. Tighe, A. N. van den Pol, L. de Lecea, H. C. Heller, J. G. Sutcliffe, and T. S. Kilduff
Neurons Containing Hypocretin (Orexin) Project to Multiple Neuronal Systems
J. Neurosci., December 1, 1998; 18(23): 9996 - 10015.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Jourdain, J.-M. Israel, B. Dupouy, S. H. R. Oliet, M. Allard, S. Vitiello, D. T. Theodosis, and D. A. Poulain
Evidence for a Hypothalamic Oxytocin-Sensitive Pattern-Generating Network Governing Oxytocin Neurons In Vitro
J. Neurosci., September 1, 1998; 18(17): 6641 - 6649.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online