JN Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 78: 3069-3076, 1997;
0022-3077/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hollins, B.
Right arrow Articles by Ikeda, S. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hollins, B.
Right arrow Articles by Ikeda, S. R.

The Journal of Neurophysiology Vol. 78 No. 6 December 1997, pp. 3069-3076
Copyright ©1997 The American Physiological Society

Heterologous Expression of a P2x-Purinoceptor in Rat Chromaffin Cells Detects Vesicular ATP Release

Bettye Hollins and Stephen R. Ikeda

Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia 30912-2300

Hollins, Bettye and Stephen R. Ikeda. Heterologous expression of a P2x-purinoceptor in rat chromaffin cells detects vesicular ATP release. J. Neurophysiol. 78: 3069-3076, 1997. A cloned P2x-purinoceptor was transiently expressed in single isolated rat adrenal chromaffin cells and evaluated for the detection of released ATP. After cytoplasmic injection of the P2x complementary RNA (cRNA; 4-24 h), application of ATP produced an inwardly rectifying current over the voltage range -130 to -10 mV as measured by the whole cell patch-clamp technique. The dose-response curve for ATP was sigmoidal with a 50% effective concentration of 18.2 µM. Suramin, a P2x-antagonist, attenuated the ATP-induced current. Depolarizing voltage pulses to 0 mV or application of histamine, stimuli that trigger exocytosis, resulted in the appearance of suramin-sensitive spontaneous transient inward currents (at -60 mV) that resembled excitatory postsynaptic currents although they were slower in time course. Concurrent detection of catecholamine release with a carbon fiber electrode often showed coincidence of the amperometric current with the synaptic currentlike events suggesting that ATP and catecholamines were released from the same vessicle. These data demonstrate that expression of a P2x-purinoceptor in chromaffin cells produces a functional autoreceptor capable of detecting vesicular release of ATP. In combination with carbon fiber amperometry, simultaneous vesicular release of two neurotransmitters from a single chromaffin cell could be monitored. The P2x-purinoceptor, however, produced a regenerative effect on release apparently resulting from the high Ca2+ permeability of the receptor. Thus modification of the P2x-purinoceptor would be required before the system could be applied to examining processes involved in stimulus-release coupling.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
H. Hirasawa, M. Puopolo, and E. Raviola
Extrasynaptic Release of GABA by Retinal Dopaminergic Neurons
J Neurophysiol, July 1, 2009; 102(1): 146 - 158.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Zhang, Y. Chen, C. Wang, and L.-Y. M. Huang
Neuronal somatic ATP release triggers neuron-satellite glial cell communication in dorsal root ganglia
PNAS, June 5, 2007; 104(23): 9864 - 9869.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev, April 1, 2007; 87(2): 659 - 797.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
M. Braun, A. Wendt, J. Karanauskaite, J. Galvanovskis, A. Clark, P. E. MacDonald, and P. Rorsman
Corelease and Differential Exit via the Fusion Pore of GABA, Serotonin, and ATP from LDCV in Rat Pancreatic {beta} Cells
J. Gen. Physiol., March 26, 2007; 129(3): 221 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. D. Tompkins and R. L. Parsons
Exocytotic release of ATP and activation of P2X receptors in dissociated guinea pig stellate neurons
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1062 - C1071.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Tolhurst, C. Vial, C. Leon, C. Gachet, R. J. Evans, and M. P. Mahaut-Smith
Interplay between P2Y1, P2Y12, and P2X1 receptors in the activation of megakaryocyte cation influx currents by ADP: evidence that the primary megakaryocyte represents a fully functional model of platelet P2 receptor signaling
Blood, September 1, 2005; 106(5): 1644 - 1651.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Fabbro, A. Skorinkin, M. Grandolfo, A. Nistri, and R. Giniatullin
Quantal release of ATP from clusters of PC12 cells
J. Physiol., October 15, 2004; 560(2): 505 - 517.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D. Gatof, G. Kilic, and J. G. Fitz
Vesicular exocytosis contributes to volume-sensitive ATP release in biliary cells
Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G538 - G546.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
M. Braun, A. Wendt, B. Birnir, J. Broman, L. Eliasson, J. Galvanovskis, J. Gromada, H. Mulder, and P. Rorsman
Regulated Exocytosis of GABA-containing Synaptic-like Microvesicles in Pancreatic {beta}-cells
J. Gen. Physiol., February 23, 2004; 123(3): 191 - 204.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. Kawa
Discrete but simultaneous release of adenine nucleotides and serotonin from mouse megakaryocytes as detected with patch- and carbon-fiber electrodes
Am J Physiol Cell Physiol, January 1, 2004; 286(1): C119 - C128.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
A. Skorinkin, A. Nistri, and R. Giniatullin
Bimodal Action of Protons on ATP Currents of Rat PC12 Cells
J. Gen. Physiol., June 30, 2003; 122(1): 33 - 44.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Reigada, I. Diez-Perez, P. Gorostiza, A. Verdaguer, I. Gomez de Aranda, O. Pineda, J. Vilarrasa, J. Marsal, J. Blasi, J. Aleu, et al.
Control of neurotransmitter release by an internal gel matrix in synaptic vesicles
PNAS, March 18, 2003; 100(6): 3485 - 3490.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. M. Schwiebert and B. K. Kishore
Extracellular nucleotide signaling along the renal epithelium
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F945 - F963.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
M. L. Cotrina and M. Nedergaard
ATP as a Messenger in Astrocyte-Neuronal Communication
Neuroscientist, April 1, 2000; 6(2): 120 - 126.
[Abstract] [PDF]

Home page
 Mol. Pharmacol.Home page
K. J. Lynch, E. Touma, W. Niforatos, K. L. Kage, E. C. Burgard, T. van Biesen, E. A. Kowaluk, and M. F. Jarvis
Molecular and Functional Characterization of Human P2X2 Receptors
Mol. Pharmacol., December 1, 1999; 56(6): 1171 - 1181.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. M. Braunstein, R. M. Roman, J. P. Clancy, B. A. Kudlow, A. L. Taylor, V. Gh. Shylonsky, B. Jovov, K. Peter, T. Jilling, I. I. Ismailov, et al.
Cystic Fibrosis Transmembrane Conductance Regulator Facilitates ATP Release by Stimulating a Separate ATP Release Channel for Autocrine Control of Cell Volume Regulation
J. Biol. Chem., February 23, 2001; 276(9): 6621 - 6630.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online