JN Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 79: 670-676, 1998;
0022-3077/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vyklický, L.
Right arrow Articles by Reeh, P. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vyklický, L.
Right arrow Articles by Reeh, P. W.

The Journal of Neurophysiology Vol. 79 No. 2 February 1998, pp. 670-676
Copyright ©1998 The American Physiological Society

Inflammatory Mediators at Acidic pH Activate Capsaicin Receptors in Cultured Sensory Neurons From Newborn Rats

Ladislav Vyklický1, Helena Knotková-Urbancová1, Zdenka Vitásková1, VIKTORIE Vlachová1, Michaela Kress2, and Peter W. Reeh2

1 Institute of Physiology, AS CR, 14220 Prague 4, Czech Republic; 2 Institut für Physiologie und Experimentelle Pathophysiologie, D-91054 Erlangen, Germany

Vyklický, Ladislav, Helena Knotková-Urbancová, Zdenka Vitásková, Viktorie Vlachová, Michaela Kress, and Peter W. Reeh. Inflammatory mediators at acidic pH activate capsaicin receptors in cultured sensory neurons from newborn rats. J. Neurophysiol. 79: 670-676, 1998. Whole cell membrane currentsinduced by the inflammatory mediators, bradykinin, 5-hydroxytryptamine (5-HT) and prostaglandin E2, were investigated in capsaicin-sensitive dorsal root ganglion (DRG) neurons from newborn rats grown on a monolayer of hippocampal glia without nerve growth factor (NGF). When firmly attached to an underlying cell, the neurons survived >14 days without growing extensive processes. A majority of the small diameter neurons (~80%) exhibited sensitivity to capsaicin (3-6 µM) and this was enhanced in solution of low pH. In acidic extracellular solution (pH 6.1), the combination of bradykinin (10 µM), 5-HT (10 µM) and prostaglandin E2 (1 µM) induced an inward membrane current in all capsaicin-sensitive DRG neurons (n = 43). The current exceeded the sustained, low pH-induced membrane current by 205 ± 53 (SE) pA. The combination of acidic inflammatory mediators was ineffective in cells that were insensitive to capsaicin. In capsaicin-sensitive neurons, the inflammatory mediators when applied singly or in any combination of two, induced no membrane currents or small current at pH 7.3 and 6.1. Capsazepine (10 µM), the capsaicin antagonist, completely inhibited the facilitatory action of inflammatory mediator combination but not the sustained inward current induced by acidic extracellular solution (pH 6.1 or 5.5). It is suggested that the inflammatory mediators, bradykinin,5-HT, and prostaglandin E2 together act as endogenous mediators at capsaicin receptors to generate an inward current when the ion channel is protonized.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
T. Fischbach, W. Greffrath, H. Nawrath, and R.-D. Treede
Effects of Anandamide and Noxious Heat on Intracellular Calcium Concentration in Nociceptive DRG Neurons of Rats
J Neurophysiol, August 1, 2007; 98(2): 929 - 938.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. Alessandri-Haber, O. A. Dina, E. K. Joseph, D. Reichling, and J. D. Levine
A transient receptor potential vanilloid 4-dependent mechanism of hyperalgesia is engaged by concerted action of inflammatory mediators.
J. Neurosci., April 5, 2006; 26(14): 3864 - 3874.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. M. Strassman and D. Levy
Response Properties of Dural Nociceptors in Relation to Headache
J Neurophysiol, March 1, 2006; 95(3): 1298 - 1306.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. McGaraughty, K. L. Chu, C. R. Faltynek, and M. F. Jarvis
Systemic and Site-Specific Effects of A-425619, a Selective TRPV1 Receptor Antagonist, on Wide Dynamic Range Neurons in CFA-Treated and Uninjured Rats
J Neurophysiol, January 1, 2006; 95(1): 18 - 25.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Sugiura, K. Dang, K. Lamb, K. Bielefeldt, and G. F. Gebhart
Acid-Sensing Properties in Rat Gastric Sensory Neurons from Normal and Ulcerated Stomach
J. Neurosci., March 9, 2005; 25(10): 2617 - 2627.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
T. Strecker, K. Messlinger, M. Weyand, and P. W. Reeh
Role of different proton-sensitive channels in releasing calcitonin gene-related peptide from isolated hearts of mutant mice
Cardiovasc Res, February 1, 2005; 65(2): 405 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Phillips, A. Reeve, S. Bevan, and P. McIntyre
Identification of Species-specific Determinants of the Action of the Antagonist Capsazepine and the Agonist PPAHV on TRPV1
J. Biol. Chem., April 23, 2004; 279(17): 17165 - 17172.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. McGaraughty, K. L. Chu, R. S. Bitner, B. Martino, R. E. Kouhen, P. Han, A. L. Nikkel, E. C. Burgard, C. R. Faltynek, and M. F. Jarvis
Capsaicin Infused Into the PAG Affects Rat Tail Flick Responses to Noxious Heat and Alters Neuronal Firing in the RVM
J Neurophysiol, October 1, 2003; 90(4): 2702 - 2710.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Zheng, C. Dai, P. S. Steyger, Y. Kim, Z. Vass, T. Ren, and A. L. Nuttall
Vanilloid Receptors in Hearing: Altered Cochlear Sensitivity by Vanilloids and Expression of TRPV1 in the Organ of Corti
J Neurophysiol, July 1, 2003; 90(1): 444 - 455.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. J. Valenzano, E. R. Grant, G. Wu, M. Hachicha, L. Schmid, L. Tafesse, Q. Sun, Y. Rotshteyn, J. Francis, J. Limberis, et al.
N-(4-Tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine -1(2H)-carbox-amide (BCTC), a Novel, Orally Effective Vanilloid Receptor 1 Antagonist with Analgesic Properties: I. In Vitro Characterization and Pharmacokinetic Properties
J. Pharmacol. Exp. Ther., July 1, 2003; 306(1): 377 - 386.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. B. Veldhuis, M. van der Stelt, M. W. Wadman, G. van Zadelhoff, M. Maccarrone, F. Fezza, G. A. Veldink, J. F. G. Vliegenthart, P. R. Bar, K. Nicolay, et al.
Neuroprotection by the Endogenous Cannabinoid Anandamide and Arvanil against In Vivo Excitotoxicity in the Rat: Role of Vanilloid Receptors and Lipoxygenases
J. Neurosci., May 15, 2003; 23(10): 4127 - 4133.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. M. Walker, L. Urban, S. J. Medhurst, S. Patel, M. Panesar, A. J. Fox, and P. McIntyre
The VR1 Antagonist Capsazepine Reverses Mechanical Hyperalgesia in Models of Inflammatory and Neuropathic Pain
J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 56 - 62.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
G. R. Seabrook, K. G. Sutton, W. Jarolimek, G. J. Hollingworth, S. Teague, J. Webb, N. Clark, S. Boyce, J. Kerby, Z. Ali, et al.
Functional Properties of the High-Affinity TRPV1 (VR1) Vanilloid Receptor Antagonist (4-Hydroxy-5-iodo-3-methoxyphenylacetate ester) Iodo-Resiniferatoxin
J. Pharmacol. Exp. Ther., December 1, 2002; 303(3): 1052 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
H. M. Himmel, T. Kiss, S. J. Borvendeg, C. Gillen, and P. Illes
The Arginine-Rich Hexapeptide R4W2 Is a Stereoselective Antagonist at the Vanilloid Receptor 1: A Ca2+ Imaging Study in Adult Rat Dorsal Root Ganglion Neurons
J. Pharmacol. Exp. Ther., June 1, 2002; 301(3): 981 - 986.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
P. A.S. Breslin, T. N. Gingrich, and B. G. Green
Ibuprofen as a Chemesthetic Stimulus: Evidence of a Novel Mechanism of Throat Irritation
Chem Senses, January 1, 2001; 26(1): 55 - 65.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. W. Hwang, H. Cho, J. Kwak, S.-Y. Lee, C.-J. Kang, J. Jung, S. Cho, K. H. Min, Y.-G. Suh, D. Kim, et al.
Direct activation of capsaicin receptors by products of lipoxygenases: Endogenous capsaicin-like substances
PNAS, May 23, 2000; 97(11): 6155 - 6160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. N. Wood
Pathobiology of Visceral Pain: Molecular Mechanisms and Therapeutic Implications: II. Genetic approaches to pain therapy
Am J Physiol Gastrointest Liver Physiol, April 1, 2000; 278(4): G507 - G512.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
A. Szallasi and P. M. Blumberg
Vanilloid (Capsaicin) Receptors and Mechanisms
Pharmacol. Rev., June 1, 1999; 51(2): 159 - 212.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Olah, L. Karai, and M. J. Iadarola
Anandamide Activates Vanilloid Receptor 1 (VR1) at Acidic pH in Dorsal Root Ganglia Neurons and Cells Ectopically Expressing VR1
J. Biol. Chem., August 10, 2001; 276(33): 31163 - 31170.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online