The Journal of Neurophysiology Vol. 79 No. 3 March 1998, pp. 1341-1348
Copyright ©1998 The American Physiological Society

Ontogenesis of Presynaptic GABA_B Receptor-Mediated Inhibition in the CA3 Region of the Rat Hippocampus

Olivier Caillard, Heather A. McLean, Yehezkel Ben-Ari, and Jean-Luc Gaïarsa

Institut National de la Santé et de la Recherche Médicale U29, Hôpital de Port-Royal, 75014 Paris, France

Caillard, Olivier, Heather A. McLean, Yehezkel Ben-Ari, and Jean-Luc Gaïarsa. Ontogenesis of presynaptic GABA_B receptor-mediated inhibition in the CA3 region of the rat hippocampus.J. Neurophysiol. 79: 1341-1348, 1998. -Aminobutyric acid-B(GABA_B) receptor-dependent and -independent components of paired-pulse depression (PPD) were investigated in the rat CA3 hippocampal region. Intracellular and whole cell recordings of CA3 pyramidal neurons were performed on hippocampal slices obtained from neonatal (5-7 day old) and adult (27-34 day old) rats. Electrical stimulation in the hilus evoked monosynaptic GABA_A postsynaptic currents (eIPSCs) isolated in the presence of the ionotropic glutamate receptor antagonists 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM) and D()2-amino-5-phosphovaleric acid (D-AP5, 50 µM) with 2(triethylamino)-N-(2,6-dimethylphenyl) acetamine (QX314) filled electrodes. In adult CA3 pyramidal neurons, when a pair of identical stimuli was applied at interstimulus intervals (ISIs) ranging from 50 to 1,500 ms the amplitude of the second eIPSC was depressed when compared with the first eIPSC. This paired-pulse depression (PPD) was partially blockedb y   P - 3 - a m i n o p r o p y l - P - d i e t h o x y m e t h y l   p h o s p h o r i c   a c i d(CGP35348, 0.5 mM), a selective GABA_B receptor antagonist. In neonates, PPD was restricted to ISIs shorter than 200 ms and was not affected by CGP35348. The GABA_B receptor agonist baclofen reduced the amplitude of eIPSCs in a dose-dependent manner with the same efficiency in both adults and neonates. Increasing the probability of transmitter release with high Ca²⁺ (4 mM)/low Mg²⁺ (0.3 mM) external solution revealed PPD in neonatal CA3 pyramidal neurons that was 1) partially prevented by CGP35348, 2) independent of the membrane holding potential of the recorded cell, and 3) not resulting from a change in the reversal potential of GABA_A eIPSCs. In adults the GABA uptake blocker tiagabine (20 µM) increased the duration of eIPSCs and the magnitude of GABA_B receptor-dependent PPD. In neonates, tiagabine also increased duration of eIPSCs but to a lesser extent than in adult and did not reveal a GABA_B receptor-dependent PPD. These results demonstrate that although GABA_B receptor-dependent and -independent mechanisms of presynaptic inhibition are present onGABAergic terminals and functional, they do not operate at the level of monosynaptic GABAergic synaptic transmission at early stages of development. Absence of presynaptic autoinhibition of GABA release seems to be due to the small amount of transmitter that can access presynaptic regulatory sites.

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