JN AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 79: 3151-3156, 1998;
0022-3077/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Linden, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Linden, D. J.

The Journal of Neurophysiology Vol. 79 No. 6 June 1998, pp. 3151-3156
Copyright ©1998 The American Physiological Society

Synaptically Evoked Glutamate Transport Currents May Be Used To Detect the Expression of Long-Term Potentiation in Cerebellar Culture

David J. Linden

Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Linden, David J. Synaptically evoked glutamate transport currents may be used to detect the expression of long-term potentiation in cerebellar culture. J. Neurophysiol. 79: 3151-3156, 1998. Cerebellar long-term potentiation (LTP) is a use-dependent increase in the strength of the granule cell-Purkinje neuron synapse that occurs after brief stimulation of granule cell axons at 2-8 Hz. Previous work has shown that cerebellar LTP also may be seen when synaptic currents are evoked in granule cell-glial cell pairs in culture. This finding suggests a model in which cerebellar LTP is expressed presynaptically and therefore may be detected by either neuronal or glial postsynaptic cells. However, synaptic currents evoked in both granule cell-glial cell pairs and granule cell-Purkinje neuron pairs in culture are mediated primarily by alpha -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate receptors, raising the possibility that cerebellar LTP might be expressed postsynaptically in both glial cells and Purkinje neurons in a similar manner. To address this question, glutamate transport currents were recorded in granule cell-glial cell pairs in culture by pharmacological isolation. These currents were increased by substitution of internal Cl with NO3 and were blocked by L-pyrrolidine-2,4-dicarboxylate, both characteristics of the major cloned Bergmann glial cell glutamate transporter, EAAT1. After acquisition of baseline responses, LTP of isolated transport current was evoked by stimulation at 4 Hz (100 pulses) and could be blocked by removal of external Ca during this stimulation. The expression of LTP was associated with a decrease in the rate of synaptic failures and a decrease in the degree of paired-pulse facilitation. These findings, when taken together with the previous observation that both Purkinje neuron and glial AMPA/kainate responses can be used to detect cerebellar LTP, strongly suggest that the expression of cerebellar LTP is, at least in part, presynaptic. This strategy should also be useful in illuminating the locus of expression of other model systems of information storage such as hippocampal LTP/long-term depression.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
W.-P. Ge and S. Duan
Persistent Enhancement of Neuron-Glia Signaling Mediated by Increased Extracellular K+ Accompanying Long-Term Synaptic Potentiation
J Neurophysiol, March 1, 2007; 97(3): 2564 - 2569.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Bayatti, H. Hermann, B. Lutz, and C. Behl
Corticotropin-Releasing Hormone-Mediated Induction of Intracellular Signaling Pathways and Brain-Derived Neurotrophic Factor Expression Is Inhibited by the Activation of the Endocannabinoid System
Endocrinology, March 1, 2005; 146(3): 1205 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Lev-Ram, S. B. Mehta, D. Kleinfeld, and R. Y. Tsien
Reversing cerebellar long-term depression
PNAS, December 23, 2003; 100(26): 15989 - 15993.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Bordey and H. Sontheimer
Modulation of Glutamatergic Transmission by Bergmann Glial Cells in Rat Cerebellum In Situ
J Neurophysiol, February 1, 2003; 89(2): 979 - 988.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Lev-Ram, S. T. Wong, D. R. Storm, and R. Y. Tsien
A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP
PNAS, June 11, 2002; 99(12): 8389 - 8393.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. J. Linden and S. Ahn
Activation of Presynaptic cAMP-Dependent Protein Kinase Is Required for Induction of Cerebellar Long-Term Potentiation
J. Neurosci., December 1, 1999; 19(23): 10221 - 10227.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Murashima and T. Hirano
Entire Course and Distinct Phases of Day-Lasting Depression of Miniature EPSC Amplitudes in Cultured Purkinje Neurons
J. Neurosci., September 1, 1999; 19(17): 7326 - 7333.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Ventura and K. M. Harris
Three-Dimensional Relationships between Hippocampal Synapses and Astrocytes
J. Neurosci., August 15, 1999; 19(16): 6897 - 6906.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online