| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Neurophysiology Vol. 80 No. 3 September 1998,
pp. 1021-1032
Copyright ©1998 The American Physiological Society
Department of Anatomy, Visual/Motor Neuroscience Division, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 23298-0709
Roberts, Elizabeth B., M. Alex Meredith, and Ary S. Ramoa. Suppression of NMDA receptor function using antisense DNA blocks ocular dominance plasticity while preserving visual responses. J. Neurophysiol. 80: 1021-1032, 1998. Pioneering work has shown that pharmacological blockade of the N-methyl-D-aspartate (NMDA) receptor channel reduces ocular dominance plasticity. However, the results also show that doses of NMDA receptor antagonists that have an effect on ocular dominance plasticity profoundly reduce sensory responses and disrupt stimulus selectivity of cortical cells. It is, therefore, not possible to determine whether effects of NMDA receptor blockade on visual plasticity result from a specific role of NMDA receptors or from the reduction in sensory response. We have used an alternate approach to examine this question. We performed knockdown experiments using antisense oligodeoxynucleotides (ODNs) complementary to mRNA coding the NR1 subunit of the NMDA receptor. After 5 days of antisense, but not sense, ODN treatment NMDA receptor-mediated synaptic transmission was reduced markedly relative to the 
-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor response, as indicated by whole cell patch-clamp recordings in the cortical slice preparation. This suppression of NMDA receptor-mediated currents was due to a selective reduction in the NR1 protein near the injection site relative to the untreated hemisphere in the same animal, as indicated by immunocytochemistry and Western blotting. In contrast, AMPA receptors were not affected by the antisense ODN treatment indicating specificity of effects. Another major effect of this treatment was to decrease ocular dominance plasticity. Ferrets that were monocularly deprived 1 wk during the antisense ODN treatment had ocular dominance histograms similar to those found in untreated, nondeprived animals. In contrast, ferrets treated with sense ODN and monocularly deprived had ocular dominance histograms resembling those of untreated, monocularly deprived animals. The effects on ocular dominance plasticity did not result from a disruption of sensory responses because maximum responses as well as orientation and direction selectivity of cortical cells were not affected by the treatment. In conclusion, the present results show that antisense techniques can accomplish more selective manipulations of cortical function than is possible with traditional pharmacological agents. Use of this approach also provides unambiguous evidence for a specific role of NMDA receptors in visual plasticity.
This article has been cited by other articles:
|
|
 |
|
  B.-J. Yoon, G. B. Smith, A. J. Heynen, R. L. Neve, and M. F. Bear Essential role for a long-term depression mechanism in ocular dominance plasticity PNAS, June 16, 2009; 106(24): 9860 - 9865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. de Marchena, A. C. Roberts, P. G. Middlebrooks, V. Valakh, K. Yashiro, L. R. Wilfley, and B. D. Philpot NMDA Receptor Antagonists Reveal Age-Dependent Differences in the Properties of Visual Cortical Plasticity J Neurophysiol, October 1, 2008; 100(4): 1936 - 1948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Bender, C. B. Allen, V. A. Bender, and D. E. Feldman Synaptic basis for whisker deprivation-induced synaptic depression in rat somatosensory cortex. J. Neurosci., April 19, 2006; 26(16): 4155 - 4165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-Y. Choi, J. Chang, B. Jiang, G.-H. Seol, S.-S. Min, J.-S. Han, H.-S. Shin, M. Gallagher, and A. Kirkwood Multiple Receptors Coupled to Phospholipase C Gate Long-Term Depression in Visual Cortex J. Neurosci., December 7, 2005; 25(49): 11433 - 11443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Jha, B. E. Jones, T. Coleman, N. Steinmetz, C.-T. Law, G. Griffin, J. Hawk, N. Dabbish, V. A. Kalatsky, and M. G. Frank Sleep-Dependent Plasticity Requires Cortical Activity J. Neurosci., October 5, 2005; 25(40): 9266 - 9274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Medina, T. E. Krahe, and A. S. Ramoa Early Alcohol Exposure Induces Persistent Alteration of Cortical Columnar Organization and Reduced Orientation Selectivity in the Visual Cortex J Neurophysiol, March 1, 2005; 93(3): 1317 - 1325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Sjostrom, G. G. Turrigiano, and S. B. Nelson Endocannabinoid-Dependent Neocortical Layer-5 LTD in the Absence of Postsynaptic Spiking J Neurophysiol, December 1, 2004; 92(6): 3338 - 3343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Yeung, H. Z. Shouval, B. S. Blais, and L. N. Cooper Synaptic homeostasis and input selectivity follow from a calcium-dependent plasticity model PNAS, October 12, 2004; 101(41): 14943 - 14948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Liao, T. E. Krahe, G. T. Prusky, A. E. Medina, and A. S. Ramoa Recovery of Cortical Binocularity and Orientation Selectivity After the Critical Period for Ocular Dominance Plasticity J Neurophysiol, October 1, 2004; 92(4): 2113 - 2121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cancedda, E. Putignano, A. Sale, A. Viegi, N. Berardi, and L. Maffei Acceleration of Visual System Development by Environmental Enrichment J. Neurosci., May 19, 2004; 24(20): 4840 - 4848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Medina, T. E. Krahe, D. M. Coppola, and A. S. Ramoa Neonatal Alcohol Exposure Induces Long-Lasting Impairment of Visual Cortical Plasticity in Ferrets J. Neurosci., November 5, 2003; 23(31): 10002 - 10012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Erisir and J. L. Harris Decline of the Critical Period of Visual Plasticity Is Concurrent with the Reduction of NR2B Subunit of the Synaptic NMDA Receptor in Layer 4 J. Neurosci., June 15, 2003; 23(12): 5208 - 5218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fagiolini, H. Katagiri, H. Miyamoto, H. Mori, S. G. N. Grant, M. Mishina, and T. K. Hensch Separable features of visual cortical plasticity revealed by N-methyl-D-aspartate receptor 2A signaling PNAS, March 4, 2003; 100(5): 2854 - 2859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Liao, A. F. Mower, R. L. Neve, C. Sato-Bigbee, and A. S. Ramoa Different Mechanisms for Loss and Recovery of Binocularity in the Visual Cortex J. Neurosci., October 15, 2002; 22(20): 9015 - 9023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Beaver, Q. S. Fischer, Q. Ji, and N. W. Daw Orientation Selectivity Is Reduced by Monocular Deprivation in Combination With PKA Inhibitors J Neurophysiol, October 1, 2002; 88(4): 1933 - 1940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Mower, D. S. Liao, E. J. Nestler, R. L. Neve, and A. S. Ramoa cAMP/Ca2+ Response Element-Binding Protein Function Is Essential for Ocular Dominance Plasticity J. Neurosci., March 15, 2002; 22(6): 2237 - 2245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Boroojerdi, F. Battaglia, W. Muellbacher, and L. G. Cohen Mechanisms underlying rapid experience-dependent plasticity in the human visual cortex PNAS, December 4, 2001; 98(25): 14698 - 14701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Huang and S. L. Pallas NMDA Antagonists in the Superior Colliculus Prevent Developmental Plasticity But Not Visual Transmission or Map Compression J Neurophysiol, September 1, 2001; 86(3): 1179 - 1194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Ramoa, A. F. Mower, D. Liao, and S. I. A. Jafri Suppression of Cortical NMDA Receptor Function Prevents Development of Orientation Selectivity in the Primary Visual Cortex J. Neurosci., June 15, 2001; 21(12): 4299 - 4309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Quinlan, D. H. Olstein, and M. F. Bear Bidirectional, experience-dependent regulation of N-methyl-D-aspartate receptor subunit composition in the rat visual cortex during postnatal development PNAS, October 26, 1999; 96(22): 12876 - 12880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. Issa, J. T. Trachtenberg, B. Chapman, K. R. Zahs, and M. P. Stryker The Critical Period for Ocular Dominance Plasticity in the Ferret's Visual Cortex J. Neurosci., August 15, 1999; 19(16): 6965 - 6978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Roberts and A. S. Ramoa Enhanced NR2A Subunit Expression and Decreased NMDA Receptor Decay Time at the Onset of Ocular Dominance Plasticity in the Ferret J Neurophysiol, May 1, 1999; 81(5): 2587 - 2591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Jablonska, M. Gierdalski, M. Kossut, and J. Skangiel-Kramska Partial Blocking of NMDA Receptors Reduces Plastic Changes Induced by Short-lasting Classical Conditioning in the SI Barrel Cortex of Adult Mice Cereb Cortex, April 1, 1999; 9(3): 222 - 231. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
