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J Neurophysiol 80: 2514-2520, 1998;
0022-3077/98 $5.00
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The Journal of Neurophysiology Vol. 80 No. 5 November 1998, pp. 2514-2520
Copyright ©1998 The American Physiological Society

Protein Kinase C Regulates a Vesicular Class of Calcium Channels in the Bag Cell Neurons of Aplysia

Benjamin H. White1, Teresa A. Nick2, Thomas J. Carew3, and Leonard K. Kaczmarek1

1 Department of Pharmacology, Yale University School of Medicine; and 2 Inderdepartmental Neuroscience Program; and 3 Departments of Psychology and Biology, Yale University, New Haven, Connecticut 06520

White, Benjamin H., Teresa A. Nick, Thomas J. Carew, and Leonard K. Kaczmarek. Protein kinase C regulates a vesicular class of calcium channels in the bag cell neurons of Aplysia. J. Neurophysiol. 80: 2514-2520, 1998. Protein kinase C (PKC) acutely increases calcium currents in Aplysia bag cell neurons by recruiting calcium channels different from those constitutively active in the plasma membrane. To study the mechanism of PKC regulation we previously identified two calcium channel alpha 1-subunits expressed in bag cell neurons. One of these, BC-alpha 1A, is localized to vesicles concentrated primarily in somata and growth cones. We used antibodies to BC-alpha 1A to analyze its expression in the bag cell neurons of juvenile Aplysia at a developmental stage at which PKC-sensitive calcium currents have previously been shown to be low. We find that vesicular BC-alpha 1A staining is generally reduced in juvenile bag cell neurons but that its expression level can vary among juvenile animals. In 17 bag cell clusters examined, the percentage of neurons that displayed punctate alpha BC-alpha 1A staining ranged from 0 to 85%. Sampling of calcium currents from cells of the same clusters by whole cell patch-clamp techniques revealed that the PKC-sensitive calcium current density is significantly correlated with the degree of vesicular staining. In contrast, no correlation of basal calcium current levels with aBC-alpha 1A staining was found. These results strongly suggest that BC-alpha 1A, a member of the ABE-subfamily of calcium channels, carries the PKC-sensitive calcium current in bag cell neurons. They are consistent with a model in which PKC recruits channels from the vesicular pool to the plasma membrane.




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