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J Neurophysiol 81: 712-721, 1999;
0022-3077/99 $5.00
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The Journal of Neurophysiology Vol. 81 No. 2 February 1999, pp. 712-721
Copyright ©1999 by the American Physiological Society

In Vivo Intracellular Analysis of Granule Cell Axon Reorganization in Epileptic Rats

Paul S. Buckmaster1 and F. Edward Dudek2

 1Department of Comparative Medicine, Stanford University School of Medicine, Stanford, California 94305-5410; and  2Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado 80523

In vivo intracellular analysis of granule cell axon reorganization in epileptic rats. In vivo intracellular recording and labeling in kainate-induced epileptic rats was used to address questions about granule cell axon reorganization in temporal lobe epilepsy. Individually labeled granule cells were reconstructed three dimensionally and in their entirety. Compared with controls, granule cells in epileptic rats had longer average axon length per cell; the difference was significant in all strata of the dentate gyrus including the hilus. In epileptic rats, at least one-third of the granule cells extended an aberrant axon collateral into the molecular layer. Axon projections into the molecular layer had an average summed length of 1 mm per cell and spanned 600 µm of the septotemporal axis of the hippocampus---a distance within the normal span of granule cell axon collaterals. These findings in vivo confirm results from previous in vitro studies. Surprisingly, 12% of the granule cells in epileptic rats, and none in controls, extended a basal dendrite into the hilus, providing another route for recurrent excitation. Consistent with recurrent excitation, many granule cells (56%) in epileptic rats displayed a long-latency depolarization superimposed on a normal inhibitory postsynaptic potential. These findings demonstrate changes, occurring at the single-cell level after an epileptogenic hippocampal injury, that could result in novel, local, recurrent circuits.

0022-3077/99 $5.00 Copyright © 1999 The American Physiological Society



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