JN Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 81: 1123-1134, 1999;
0022-3077/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, H.-C.
Right arrow Articles by Chung, M.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, H.-C.
Right arrow Articles by Chung, M.-K.

The Journal of Neurophysiology Vol. 81 No. 3 March 1999, pp. 1123-1134
Copyright ©1999 by the American Physiological Society

Voltage-Dependent Sodium and Calcium Currents in Acutely Isolated Adult Rat Trigeminal Root Ganglion Neurons

Hyung-Chan Kim1,2 and Man-Kyo Chung1

 1Department of Oral Physiology and Institute of Oral Biology, School of Dentistry, and  2East-West Medical Research Institute, Kyung Hee University, Seoul 130-701, Korea

Kim, Hyung-Chan and Man-Kyo Chung. Voltage-dependent sodium and calcium currents in acutely isolated adult rat trigeminal root ganglion neurons. Voltage-dependent sodium (INa) and calcium (ICa) currents in small (<30 µM) neurons from adult rat trigeminal root ganglia were characterized with a standard whole cell patch-clamp technique. Two types of INa showing different sensitivity to tetrodotoxin (TTX) were recorded, which showed marked differences in their activating and inactivating time courses. The activation and the steady-state inactivation kinetics of TTX-resistant INa were more depolarized by about +20 and +30 mV, respectively, than those of TTX-sensitive INa. Voltage-dependent ICa was recorded under the condition that suppressed sodium and potassium currents with 10 mM Ca2+ as a charge carrier. Depolarizing step pulses from a holding potential of -80 mV evoked two distinct inward ICa, low-voltage activated (LVA) and high-voltage activated (HVA) ICa. LVA ICa was first observed at -60 to -50 mV and reached a peak at about -30 mV. Amiloride (0.5 mM) suppressed ~60% of the LVA ICa, whereas ~10% of HVA ICa was inhibited by the same concentration of the amiloride. LVA ICa was far less affected by the presence of external Cd2+ or the replacement of Ca2+ by 10 Ba2+ than HVA ICa. The omega -conotoxin GVIA (omega -CgTx), an N-type ICa blocker, suppressed ~65% of the whole cell HVA ICa at the concentration of 1 µM. The omega -CgTx-resistant HVA ICa was sensitive to nifedipine (10 µM), a dihydropyridine (DHP) calcium channel antagonist, which produced an additional blockade by ~25% of the drug-free control (~70% of the omega -CgTx-resistant ICa). The combination of 10 µM nifedipine and 1 µM omega -CgTx left ~13% of the drug-free control ICa unblocked. The DHP agonist S(-)-BayK8644 (5 µM) shifted the activation of the HVA ICa to more negative potentials and increased its maximal amplitude. Additionally, S(-)-BayK8644 caused the appearance of a slowed component of the tail current. These results clearly demonstrate that the presence of two types of sodium channels, TTX sensitive and resistant, and three types of calcium channels, T, L, and N type, in the small-sized adult rat trigeminal ganglion neurons.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
M. D'Arco, R. Giniatullin, M. Simonetti, A. Fabbro, A. Nair, A. Nistri, and E. Fabbretti
Neutralization of Nerve Growth Factor Induces Plasticity of ATP-Sensitive P2X3 Receptors of Nociceptive Trigeminal Ganglion Neurons
J. Neurosci., August 1, 2007; 27(31): 8190 - 8201.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F.-S. Lo and R. S. Erzurumlu
Conversion of Functional Synapses into Silent Synapses in the Trigeminal Brainstem after Neonatal Peripheral Nerve Transection
J. Neurosci., May 2, 2007; 27(18): 4929 - 4934.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y. Yaari, C. Yue, and H. Su
Recruitment of apical dendritic T-type Ca2+ channels by backpropagating spikes underlies de novo intrinsic bursting in hippocampal epileptogenesis
J. Physiol., April 15, 2007; 580(2): 435 - 450.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
T. D. Gover, T. H. V. Moreira, J. P. Y. Kao, and D. Weinreich
Calcium regulation in individual peripheral sensory nerve terminals of the rat
J. Physiol., January 15, 2007; 578(2): 481 - 490.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Liu, T. M. Yang, W. Liedtke, and S. A. Simon
Chronic IL-1beta Signaling Potentiates Voltage-Dependent Sodium Currents in Trigeminal Nociceptive Neurons
J Neurophysiol, March 1, 2006; 95(3): 1478 - 1490.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Liu, T. Yang, M. J. Bruno, O. S. Andersen, and S. A. Simon
Voltage-Gated Ion Channels in Nociceptors: Modulation by cGMP
J Neurophysiol, October 1, 2004; 92(4): 2323 - 2332.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Liu, W. Zhu, Z.-S. Zhang, T. Yang, A. Grant, G. Oxford, and S. A. Simon
Nicotine Inhibits Voltage-Dependent Sodium Channels and Sensitizes Vanilloid Receptors
J Neurophysiol, April 1, 2004; 91(4): 1482 - 1491.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Grigaliunas, R. M. Bradley, D. K. MacCallum, and C. M. Mistretta
Distinctive Neurophysiological Properties of Embryonic Trigeminal and Geniculate Neurons in Culture
J Neurophysiol, October 1, 2002; 88(4): 2058 - 2074.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. Tyrrell, M. Renganathan, S. D. Dib-Hajj, and S. G. Waxman
Glycosylation Alters Steady-State Inactivation of Sodium Channel Nav1.9/NaN in Dorsal Root Ganglion Neurons and Is Developmentally Regulated
J. Neurosci., December 15, 2001; 21(24): 9629 - 9637.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Liu, M. Oortgiesen, L. Li, and S. A. Simon
Capsaicin Inhibits Activation of Voltage-Gated Sodium Currents in Capsaicin-Sensitive Trigeminal Ganglion Neurons
J Neurophysiol, February 1, 2001; 85(2): 745 - 758.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online