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The Journal of Neurophysiology Vol. 81 No. 4 April 1999, pp. 1495-1505
Copyright ©1999 by the American Physiological Society
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010
Koenig, J. H. and
Kazuo Ikeda.
Contribution of active zone subpopulation of vesicles to evoked and
spontaneous release. Our previous work on Drosophila
synapses has suggested that two vesicle populations possessing
different recycling pathways, a fast pathway emanating from the active
zone and a slower pathway emanating from sites away from the active zone, exist in the terminal. The difference in recycling time between
these two pathways has allowed us to create a synapse that possesses
the small, active zone subpopulation without the larger, nonactive zone
population. Synapses were depleted using the temperature-sensitive
endocytosis mutant, shibire, which reversibly blocks vesicle
recycling at the restrictive temperature. In the depleted state, both
the excitatory junction potential (EJP) and spontaneous release are
abolished. After shibire-induced depletion, the active zone
population begins to reform within 30 s at the permissive temperature,
whereas the nonactive zone population does not begin to reform until
~10-15 min later. Evoked release recovered at approximately the same
time as the active zone population. During the time when the active
zone population existed in the terminal without the nonactive zone
population, enough transmitter release was available to sustain a
normal evoked response for many minutes at frequencies above those
produced during normal activity (flight) by this motor neuron. When
only the active zone population existed in the terminal, the frequency
of spontaneous release was greatly attenuated and possessed abnormal
release characteristics. Spontaneous release recovered its predepletion frequency and release characteristics only after the nonactive zone
population was reformed.
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