JN AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 81: 1495-1505, 1999;
0022-3077/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koenig, J. H.
Right arrow Articles by Ikeda, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koenig, J. H.
Right arrow Articles by Ikeda, K.

The Journal of Neurophysiology Vol. 81 No. 4 April 1999, pp. 1495-1505
Copyright ©1999 by the American Physiological Society

Contribution of Active Zone Subpopulation of Vesicles to Evoked and Spontaneous Release

J. H. Koenig and Kazuo Ikeda

Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010

Koenig, J. H. and Kazuo Ikeda. Contribution of active zone subpopulation of vesicles to evoked and spontaneous release. Our previous work on Drosophila synapses has suggested that two vesicle populations possessing different recycling pathways, a fast pathway emanating from the active zone and a slower pathway emanating from sites away from the active zone, exist in the terminal. The difference in recycling time between these two pathways has allowed us to create a synapse that possesses the small, active zone subpopulation without the larger, nonactive zone population. Synapses were depleted using the temperature-sensitive endocytosis mutant, shibire, which reversibly blocks vesicle recycling at the restrictive temperature. In the depleted state, both the excitatory junction potential (EJP) and spontaneous release are abolished. After shibire-induced depletion, the active zone population begins to reform within 30 s at the permissive temperature, whereas the nonactive zone population does not begin to reform until ~10-15 min later. Evoked release recovered at approximately the same time as the active zone population. During the time when the active zone population existed in the terminal without the nonactive zone population, enough transmitter release was available to sustain a normal evoked response for many minutes at frequencies above those produced during normal activity (flight) by this motor neuron. When only the active zone population existed in the terminal, the frequency of spontaneous release was greatly attenuated and possessed abnormal release characteristics. Spontaneous release recovered its predepletion frequency and release characteristics only after the nonactive zone population was reformed.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Kawasaki and R. W. Ordway
Molecular mechanisms determining conserved properties of short-term synaptic depression revealed in NSF and SNAP-25 conditional mutants
PNAS, August 25, 2009; 106(34): 14658 - 14663.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Vijayakrishnan, E. A. Woodruff III, and K. Broadie
Rolling blackout is required for bulk endocytosis in non-neuronal cells and neuronal synapses
J. Cell Sci., January 1, 2009; 122(1): 114 - 125.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T.-W. Koh, V. I. Korolchuk, Y. P. Wairkar, W. Jiao, E. Evergren, H. Pan, Y. Zhou, K. J.T. Venken, O. Shupliakov, I. M. Robinson, et al.
Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development
J. Cell Biol., July 10, 2007; 178(2): 309 - 322.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. H. Koenig and K. Ikeda
Release and Recycling of the Readily Releasable Vesicle Population in a Synapse Possessing No Reserve Population
J Neurophysiol, June 1, 2007; 97(6): 4048 - 4057.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Glitsch
Selective Inhibition of Spontaneous But Not Ca2+-Dependent Release Machinery by Presynaptic Group II mGluRs in Rat Cerebellar Slices
J Neurophysiol, July 1, 2006; 96(1): 86 - 96.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H.-F. Guo and Y. Zhong
Requirement of Akt to Mediate Long-Term Synaptic Depression in Drosophila
J. Neurosci., April 12, 2006; 26(15): 4004 - 4014.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
E. T. Kavalali
Synaptic Vesicle Reuse and Its Implications
Neuroscientist, February 1, 2006; 12(1): 57 - 66.
[Abstract] [PDF]

Home page
 J. Neurophysiol.Home page
J. H. Koenig and K. Ikeda
Relationship of the Reserve Vesicle Population to Synaptic Depression in the Tergotrochanteral and Dorsal Longitudinal Muscles of Drosophila
J Neurophysiol, September 1, 2005; 94(3): 2111 - 2119.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
D. A. Richards, J. Bai, and E. R. Chapman
Two modes of exocytosis at hippocampal synapses revealed by rate of FM1-43 efflux from individual vesicles
J. Cell Biol., March 14, 2005; 168(6): 929 - 939.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. W. Hendricson, J. R. Sibbald, and R. A. Morrisett
Ethanol Alters the Frequency, Amplitude, and Decay Kinetics of Sr2+-Supported, Asynchronous NMDAR mEPSCs in Rat Hippocampal Slices
J Neurophysiol, June 1, 2004; 91(6): 2568 - 2577.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. T. Macleod, L. Marin, M. P. Charlton, and H. L. Atwood
Synaptic Vesicles: Test for a Role in Presynaptic Calcium Regulation
J. Neurosci., March 10, 2004; 24(10): 2496 - 2505.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. J Royle and L. Lagnado*
Endocytosis at the synaptic terminal
J. Physiol., December 1, 2003; 553(2): 345 - 355.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Fabian-Fine, P. Verstreken, P. R. Hiesinger, J. A. Horne, R. Kostyleva, Y. Zhou, H. J. Bellen, and I. A. Meinertzhagen
Endophilin Promotes a Late Step in Endocytosis at Glial Invaginations in Drosophila Photoreceptor Terminals
J. Neurosci., November 19, 2003; 23(33): 10732 - 10744.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. Wucherpfennig, M. Wilsch-Brauninger, and M. Gonzalez-Gaitan
Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release
J. Cell Biol., May 12, 2003; 161(3): 609 - 624.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Zhao and M. Klein
Modulation of the Readily Releasable Pool of Transmitter and of Excitation-Secretion Coupling by Activity and by Serotonin at Aplysia Sensorimotor Synapses in Culture
J. Neurosci., December 15, 2002; 22(24): 10671 - 10679.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Congar and L.-E. Trudeau
Perturbation of synaptic vesicle delivery during neurotransmitter release triggered independently of calcium influx
J. Physiol., August 1, 2002; 542(3): 779 - 793.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
F.-M. Lu and K. Kuba
Synchronous and Asynchronous Exocytosis Induced by Subthreshold High K+ at Cs+-Loaded Terminals of Rat Hippocampal Neurons
J Neurophysiol, March 1, 2002; 87(3): 1222 - 1233.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Teng and R. S. Wilkinson
Clathrin-Mediated Endocytosis near Active Zones in Snake Motor Boutons
J. Neurosci., November 1, 2000; 20(21): 7986 - 7993.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Bean, S. Davanger, M. F. Chou, B. Gerhardt, S. Tsujimoto, and Y. Chang
Hrs-2 Regulates Receptor-mediated Endocytosis via Interactions with Eps15
J. Biol. Chem., May 12, 2000; 275(20): 15271 - 15278.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online