The Journal of Neurophysiology Vol. 81 No. 5 May 1999, pp. 2386-2397
Copyright ©1999 by the American Physiological Society

Block of Quantal End-Plate Currents of Mouse Muscle by Physostigmine and Procaine

 ¹Physiologisches Institut and  ²Neurologische Klinik, Technische Universität München, 80802 Munich, Germany; and  ³Department of Neurobiology, Hebrew University, Jerusalem 91904, Israel

Dudel, J., M. Schramm, C. Franke, E. Ratner, and H. Parnas. Block of Quantal End-Plate Currents of Mouse Muscle by Physostigmine and Procaine. J. Neurophysiol. 81: 2386-2397, 1999.of quantal end-plate currents of mouse muscle by physostigmine and procaine. Quantal endplate currents (qEPCs) were recorded from hemidiaphragms of mice by means of a macro-patch-clamp electrode. Excitation was blocked with tetrodotoxin, and quantal release was elicited by depolarizing pulses through the electrode. Physostigmine (Phys) or procaine (Proc) was applied to the recording site by perfusion of the electrode tip. Low concentrations of Phys increased the amplitude and prolonged the decay time constants of qEPCs from ~3 to ~10 ms, due to block of acetylcholine-esterase. With 20 µM to 2 mM Phys or Proc, the decay of qEPCs became biphasic, an initial short time constant _s decreasing to <1 ms with 1 mM Phys and to ~0.3 ms with 1 mM Proc. The long second time constant of the decay, _l, reached values of 100 ms with these blocker concentrations. The blocking effects of Phys and Proc on the qEPC are due to binding to the open channel conformation. A method is described to extract the rate constants of binding (b_p) from the sums 1/_s + 1/_l, and the rates of unbinding (b_p) from ₀ · _s¹ · _l¹ (₀ is the decay time constant of the control EPC). For Phys and Proc b_p of 1.3 and 5 · 10⁶ M¹ s¹ and b_p of 176 and 350 s¹, respectively, were found. Using these rate constants and a reaction scheme for the nicotinic receptor together with the respective rate constants determined before, we could model the experimental results satisfactorily.