JN Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 81: 2508-2516, 1999;
0022-3077/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pisani, A.
Right arrow Articles by Bernardi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pisani, A.
Right arrow Articles by Bernardi, G.

The Journal of Neurophysiology Vol. 81 No. 5 May 1999, pp. 2508-2516
Copyright ©1999 by the American Physiological Society

Electrophysiological Recordings and Calcium Measurements in Striatal Large Aspiny Interneurons in Response to Combined O2/Glucose Deprivation

Antonio Pisani,1 Paolo Calabresi,1 Diego Centonze,1 Girolama A. Marfia,1 and Giorgio Bernardi1,2

 1Clinica Neurologica, Dipartimento di Neuroscienze, Università di Roma Tor Vergata, 00133 Rome;  2IRCCS Ospedale S. Lucia, 00173 Rome, Italy

Pisani, Antonio, Paolo Calabresi, Diego Centonze, Girolama A. Marfia, and Giorgio Bernardi. Electrophysiological Recordings and Calcium Measurements in Striatal Large Aspiny Interneurons in Response to Combined O2/Glucose Deprivation. J. Neurophysiol. 81: 2508-2516, 1999.Electrophysiological recordings and calcium measurements in striatal large aspiny interneurons in response to combined O2/glucose deprivation. The effects of combined O2/glucose deprivation were investigated on large aspiny (LA) interneurons recorded from a striatal slice preparation by means of simultaneous electrophysiological and optical recordings. LA interneurons were visually identified and impaled with sharp microelectrodes loaded with the calcium (Ca2+)-sensitive dye bis-fura-2. These cells showed the morphological, electrophysiological, and pharmacological features of large striatal cholinergic interneurons. O2/glucose deprivation induced a membrane hyperpolarization coupled to a concomitant increase in intracellular Ca2+ concentration ([Ca2+]i). Interestingly, this [Ca2+]i elevation was more pronounced in dendritic branches rather than in the somatic region. The O2/glucose-deprivation-induced membrane hyperpolarization reversed its polarity at the potassium (K+) equilibrium potential. Both membrane hyperpolarization and [Ca2+]i rise were unaffected by TTX or by a combination of ionotropic glutamate receptors antagonists, D-2-amino-5-phosphonovaleric acid and 6cyano-7-nitroquinoxaline-2,3-dione. Sulfonylurea glibenclamide, a blocker of ATP-sensitive K+ channels, markedly reduced the O2/glucose-deprivation-induced membrane hyperpolarization but failed to prevent the rise in [Ca2+]i. Likewise, charybdotoxin, a large K+-channel (BK) inhibitor, abolished the membrane hyperpolarization but did not produce detectable changes of [Ca2+]i elevation. A combination of high-voltage-activated Ca2+ channel blockers significantly reduced both the membrane hyperpolarization and the rise in [Ca2+]i. In a set of experiments performed without dye in the recording electrode, either intracellular bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid or external barium abolished the membrane hyperpolarization induced by O2/glucose deprivation. The hyperpolarizing effect on membrane potential was mimicked by oxotremorine, an M2-like muscarinic receptor agonist, and by baclofen, a GABAB receptor agonist. However, this membrane hyperpolarization was not coupled to an increase but rather to a decrease of the basal [Ca2+]i. Furthermore glibenclamide did not reduce the oxotremorine- and baclofen-induced membrane hyperpolarization. In conclusion, the present results suggest that in striatal LA cells, O2/glucose deprivation activates a membrane hyperpolarization that does not involve ligand-gated K+ conductances but is sensitive to barium, glibenclamide, and charybdotoxin. The increase in [Ca2+]i is partially due to influx through voltage-gated high-voltage-activated Ca2+ channels.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
P. Bonsi, G. Martella, D. Cuomo, P. Platania, G. Sciamanna, G. Bernardi, J. Wess, and A. Pisani
Loss of Muscarinic Autoreceptor Function Impairs Long-Term Depression But Not Long-Term Potentiation in the Striatum
J. Neurosci., June 11, 2008; 28(24): 6258 - 6263.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Pisani, P. Bonsi, D. Centonze, A. Martorana, F. Fusco, G. Sancesario, C. De Persis, G. Bernardi, and P. Calabresi
Activation of {beta}1-Adrenoceptors Excites Striatal Cholinergic Interneurons through a cAMP-Dependent, Protein Kinase-Independent Pathway
J. Neurosci., June 15, 2003; 23(12): 5272 - 5282.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Z.-P. Pang, P. Deng, Y.-W. Ruan, and Z. C. Xu
Depression of Fast Excitatory Synaptic Transmission in Large Aspiny Neurons of the Neostriatum after Transient Forebrain Ischemia
J. Neurosci., December 15, 2002; 22(24): 10948 - 10957.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Pisani, P. Bonsi, M. V. Catania, R. Giuffrida, M. Morari, M. Marti, D. Centonze, G. Bernardi, A. E. Kingston, and P. Calabresi
Metabotropic Glutamate 2 Receptors Modulate Synaptic Inputs and Calcium Signals in Striatal Cholinergic Interneurons
J. Neurosci., July 15, 2002; 22(14): 6176 - 6185.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
P. Calabresi, E. Saulle, D. Centonze, A. Pisani, G. A. Marfia, and G. Bernardi
Post-ischaemic long-term synaptic potentiation in the striatum: a putative mechanism for cell type-specific vulnerability
Brain, April 1, 2002; 125(4): 844 - 860.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
P. Calabresi, E. Saulle, G. A. Marfia, D. Centonze, R. Mulloy, B. Picconi, R. A. Hipskind, F. Conquet, and G. Bernardi
Activation of Metabotropic Glutamate Receptor Subtype 1/Protein Kinase C/Mitogen-Activated Protein Kinase Pathway Is Required for Postischemic Long-Term Potentiation in the Striatum
Mol. Pharmacol., October 1, 2001; 60(4): 808 - 815.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Centonze, A. Pisani, P. Bonsi, P. Giacomini, G. Bernardi, and P. Calabresi
Stimulation of Nitric Oxide-cGMP Pathway Excites Striatal Cholinergic Interneurons via Protein Kinase G Activation
J. Neurosci., February 15, 2001; 21(4): 1393 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. D. Bennett, J. C. Callaway, and C. J. Wilson
Intrinsic Membrane Properties Underlying Spontaneous Tonic Firing in Neostriatal Cholinergic Interneurons
J. Neurosci., November 15, 2000; 20(22): 8493 - 8503.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
P. Calabresi, D. Centonze, and G. Bernardi
Cellular factors controlling neuronal vulnerability in the brain: A lesson from the striatum
Neurology, November 14, 2000; 55(9): 1249 - 1255.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Kulik, S. Trapp, and K. Ballanyi
Ischemia But Not Anoxia Evokes Vesicular and Ca2+-Independent Glutamate Release In the Dorsal Vagal Complex In Vitro
J Neurophysiol, May 1, 2000; 83(5): 2905 - 2915.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Pisani, P. Bonsi, D. Centonze, P. Calabresi, and G. Bernardi
Activation of D2-Like Dopamine Receptors Reduces Synaptic Inputs to Striatal Cholinergic Interneurons
J. Neurosci., April 1, 2000; 20(7): RC69 - RC69.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online