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The Journal of Neurophysiology Vol. 81 No. 6 June 1999, pp. 2787-2797
Copyright ©1999 by the American Physiological Society
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California, 94305-5345
Pavlidis, Paul and
Daniel V. Madison.
Synaptic Transmission in Pair Recordings From CA3 Pyramidal Cells
in Organotypic Culture. J. Neurophysiol. 81: 2787-2797, 1999.
Synaptic transmission in pair recordings from CA3 pyramidal cells in
organotypic culture. We performed simultaneous whole cell
recordings from pairs of monosynaptically coupled hippocampal CA3
pyramidal neurons in organotypic slices. Stimulation of an action
potential in a presynaptic cell resulted in an AMPA-receptor-mediated excitatory postsynaptic current (EPSC) in the postsynaptic cell that
averaged ~34 pA. The average size of EPSCs varied in amplitude over a
20-fold range across different pairs. Both paired-pulse facilitation
and depression were observed in the synaptic current in response to two
presynaptic action potentials delivered 50 ms apart, but the average
usually was dominated by depression. In addition, the amplitude of the
second EPSC depended on the amplitude of the first EPSC, indicating
competition between successive events for a common resource that is not
restored within the 50-ms interpulse interval. Variation in the
synaptic strength among pairs could arise from a variety of sources.
Our data from anatomic reconstruction, 1/CV2 analysis,
paired-pulse analysis, and manipulations of calcium/magnesium ratio
suggest that differences in quantal size and release probability do not
appear to vary sufficiently to fully account for the observed differences in amplitude. Thus it seems most likely that the
variability in EPSC amplitude between pairs arises primarily from
differences in the number of functional synapses. Injections of the
calcium chelator
bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid
into the presynaptic neuron resulted in a rapid and nearly complete block of transmission, whereas injection of the slower-acting chelator
EGTA resulted in a variable and partial block. In addition to
demonstrating the feasibility of manipulating the intracellular presynaptic environment by injection into the presynaptic soma, these
data, and the EGTA results in particular may suggest variability in the
linkage between calcium entry sites an release sites in these synapses.
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