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The Journal of Neurophysiology Vol. 81 No. 6 June 1999, pp. 2852-2861
Copyright ©1999 by the American Physiological Society
Centre for Research in Neuroscience, McGill University and Montreal General Hospital Research Institute, Montreal, Quebec H3G 1A4, Canada
Nguyen, Peter V.,
Laurent Aniksztejn,
Stefano Catarsi, and
Pierre Drapeau.
Maturation of Neuromuscular Transmission During Early Development
in Zebrafish. J. Neurophysiol. 81: 2852-2861, 1999.
Maturation of neuromuscular transmission during early development in
zebrafish. We have examined the rapid development of synaptic
transmission at the neuromuscular junction (NMJ) in zebrafish embryos
and larvae by patch-clamp recording of spontaneous miniature endplate
currents (mEPCs) and single acetylcholine receptor (AChR) channels.
Embryonic (24-36 h) mEPCs recorded in vivo were small in amplitude
(<50 pA). The rate of mEPCs increased in larvae (3.5-fold increase
measured by 6 days), and these mEPCs were mostly of larger amplitude
(10-fold on average) with (
5-fold) faster kinetics. Intracellular
labeling with Lucifer yellow indicated extensive coupling between
muscle cells in both embryos and larvae (10 days). Blocking
acetylcholinesterase (AChE) with eserine had no effect on mEPC kinetics
in embryos at 1 day and only partially slowed (by ~1/2) the
decay rate in larvae at 6 days. In acutely dissociated muscle cells, we
observed the same two types of AChR with conductances of 45 and 60 pS
and with similar, brief (<0.5 ms) mean open times in both embryos and
larvae. We conclude that AChR properties are set early during
development at these early stages; functional maturation of the NMJ is
only partly shaped by expression of AChE and may also depend on
postsynaptic AChR clustering and presynaptic maturation.
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