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The Journal of Neurophysiology Vol. 82 No. 1 July 1999, pp. 312-319
Copyright ©1999 by the American Physiological Society
1The Synaptic Structure and Function Group, Division of Neuroscience, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 0200, Australia; and 2Department of Anatomy, Wright State University, Dayton, Ohio
Oleskevich, Sharon,
Francisco J. Alvarez, and
Bruce Walmsley.
Glycinergic Miniature Synaptic Currents and Receptor Cluster
Sizes Differ Between Spinal Cord Interneurons. J. Neurophysiol. 82: 312-319, 1999.
The structural
features of a synaptic connection between central neurons play an
important role in determining the strength of the connection. In the
present study, we have examined the relationship between the structural
and functional properties of glycinergic synapses in the rat spinal
cord. We have analyzed the structure of glycinergic receptor clusters
on rat ventral horn interneurons using antibodies against the glycine
receptor clustering protein, gephyrin. We have examined the properties of quantal glycinergic currents generated at these synapses using whole
cell patch-clamp recordings of miniature postsynaptic inhibitory currents (mIPSCs) in rat spinal cord slices in vitro. Our
immunolabeling results demonstrate that there is a considerable
variability in the size of glycine receptor clusters within individual
neurons. Furthermore there are large differences in the mean cluster
size between neurons. These observations are paralleled closely by recordings of glycinergic mIPSCs. The mIPSC amplitude varies
significantly within and between neurons. Results obtained using
combined immunolabeling and electrophysiological recording on the same
neurons show that cells with small glycine receptor clusters
concurrently exhibit small mIPSCs. Our results suggest that the
differences in the size of glycinergic receptor clusters may constitute
an important factor contributing to the observed differences in mIPSC
amplitude among spinal cord interneurons.
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