| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Neurophysiology Vol. 82 No. 6 December 1999, pp. 3160-3167
Copyright ©1999 by the American Physiological Society
Department of Physiology, University of Utah School of Medicine, Salt Lake City, Utah 84108-1270
Lipschitz, D. L. and
W. C. Michel.
Physiological Evidence for the Discrimination of
L-Arginine From Structural Analogues by the Zebrafish
Olfactory System. J. Neurophysiol. 82: 3160-3167, 1999. Although it is generally assumed that fish are capable of
discriminating amino acid odorants on the basis of differences in side-chain structure, less is known about their ability to discriminate amino acids with modifications to 
-carboxyl and -amino groups. In
this study, the ability of the zebrafish olfactory system to detect and
presumably discriminate analogues of the basic amino acid Arg was
assessed, by using cross-adaptation and activity-dependent labeling
techniques. Electrophysiological recordings established that
esterification (L-arginine methyl ester; AME) or deletion (agmatine or amino-4-guanidobutane; AGB) of the -carboxyl group yielded odorants more potent than Arg, whereas deletion of the -amino group (L-argininic acid; AA) yielded a less
potent analogue. In cross-adaptation experiments, no test-competitor
odorant combination yielded complete cross-adaptation, suggesting the
detection of these Arg analogues by multiple odorant receptors (ORs)
with partially nonoverlapping specificities. Activity-dependent
immunocytochemical labeling of olfactory receptor neurons supported
this conclusion. AGB, an ion-channel-permeant probe (and odorant),
labeled 4.9 ± 0.4% (n = 24) of
sensory epithelium, whereas the addition of Arg, 1-ethylguanidine
sulfate, L--amino-
-guanidinopropionate, or AME to AGB resulted in a significant elevation of labeling (8-14%). This study provides evidence that the olfactory system has
the potential to discriminate among amino acid odorants with modified
-carboxyl and -amino groups.
This article has been cited by other articles:
|
|
 |
|
  R. P. Ferrer and R. K. Zimmer The scent of danger: arginine as an olfactory cue of reduced predation risk J. Exp. Biol., May 15, 2007; 210(10): 1768 - 1775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Ferrer and R. K. Zimmer Chemosensory reception, behavioral expression, and ecological interactions at multiple trophic levels J. Exp. Biol., May 15, 2007; 210(10): 1776 - 1785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Lindsay and R. G. Vogt Behavioral Responses of Newly Hatched Zebrafish (Danio rerio) to Amino Acid Chemostimulants Chem Senses, February 1, 2004; 29(2): 93 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. Michel, M. J. Sanderson, J. K. Olson, and D. L. Lipschitz Evidence of a novel transduction pathway mediating detection of polyamines by the zebrafish olfactory system J. Exp. Biol., May 15, 2003; 206(10): 1697 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.C. Hubbard, E.N. Barata, and A.V.M. Canario Olfactory Sensitivity to Catecholamines and their Metabolites in the Goldfish Chem Senses, March 1, 2003; 28(3): 207 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Lipschitz and W. C. Michel Amino Acid Odorants Stimulate Microvillar Sensory Neurons Chem Senses, March 1, 2002; 27(3): 277 - 286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sato and N. Suzuki Whole-cell Response Characteristics of Ciliated and Microvillous Olfactory Receptor Neurons to Amino Acids, Pheromone Candidates and Urine in Rainbow Trout Chem Senses, November 1, 2001; 26(9): 1145 - 1156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Wilson Comparison of Odor Receptive Field Plasticity in the Rat Olfactory Bulb and Anterior Piriform Cortex J Neurophysiol, December 1, 2000; 84(6): 3036 - 3042. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
