JN Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 82: 3298-3306, 1999;
0022-3077/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shen, W.
Right arrow Articles by Slaughter, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shen, W.
Right arrow Articles by Slaughter, M. M.

The Journal of Neurophysiology Vol. 82 No. 6 December 1999, pp. 3298-3306
Copyright ©1999 by the American Physiological Society

Internal Calcium Modulates Apparent Affinity of Metabotropic GABA Receptors

Wen Shen and Malcolm M. Slaughter

Departments of Physiology and Biophysics and Ophthalmology, School of Medicine, State University of New York, Buffalo, New York 14214

Shen, Wen and Malcolm M. Slaughter. Internal Calcium Modulates Apparent Affinity of Metabotropic GABA Receptors. J. Neurophysiol. 82: 3298-3306, 1999. The metabotropic GABA receptor (GABABR) regulates calcium influx in neurons. Whole cell voltage-clamp techniques were employed to determine the effects of internal calcium on the activity of GABABRs. GABABR receptor apparent affinity was maximal when bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid (BAPTA) maintained internal calcium below 70 nM. Apparent affinity was reduced as internal calcium increased. EGTA did not produce similar effects, suggesting that localized increases in calcium influenced GABABR apparent affinity. Confocal imaging disclosed relatively high internal calcium just below the plasma membrane of isolated neurons. BAPTA, but not EGTA, reduced this ring of high calcium. Heparin, dantrolene, and ryanodine increased GABABR apparent affinity, effects similar to that of BAPTA. Calmodulin inhibitors also increased receptor apparent affinity. These results suggest that internally released calcium activates calmodulin, which reduces GABABR apparent affinity. This identifies a reciprocal system in which the metabotropic GABA receptor can reduce calcium influx, but internal calcium can suppress this receptor pathway. Metabotropic glutamate receptors linked to inositol 1,4,5 trisphosphate (InsP3) raised internal calcium and suppressed the action of GABABRs. Thus negative feedback systems control the balance between excitatory and inhibitory metabotropic receptor pathways in retinal neurons.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
N. Karpuk and A. Hayar
Activation of Postsynaptic GABAB Receptors Modulates the Bursting Pattern and Synaptic Activity of Olfactory Bulb Juxtaglomerular Neurons
J Neurophysiol, January 1, 2008; 99(1): 308 - 319.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Cristofanilli and A. Akopian
Calcium channel and glutamate receptor activities regulate actin organization in salamander retinal neurons
J. Physiol., September 1, 2006; 575(2): 543 - 554.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y.-C. Yu, L.-H. Cao, and X.-L. Yang
Modulation by Brain Natriuretic Peptide of GABA Receptors on Rat Retinal ON-Type Bipolar Cells
J. Neurosci., January 11, 2006; 26(2): 696 - 707.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. W. Xu and M. M. Slaughter
Large-Conductance Calcium-Activated Potassium Channels Facilitate Transmitter Release in Salamander Rod Synapse
J. Neurosci., August 17, 2005; 25(33): 7660 - 7668.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
P. Koulen, J. Wei, C. Madry, J. Liu, and E. Nixon
Differentially Distributed IP3 Receptors and Ca2+ Signaling in Rod Bipolar Cells
Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 292 - 298.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Verkhratsky
Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons
Physiol Rev, January 1, 2005; 85(1): 201 - 279.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Akopian and P. Witkovsky
Intracellular calcium reduces light-induced excitatory post-synaptic responses in salamander retinal ganglion cells
J. Physiol., April 1, 2001; 532(1): 43 - 53.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online