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J Neurophysiol 82: 3406-3416, 1999;
0022-3077/99 $5.00
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The Journal of Neurophysiology Vol. 82 No. 6 December 1999, pp. 3406-3416
Copyright ©1999 by the American Physiological Society

Role of alpha -SNAP in Promoting Efficient Neurotransmission at the Crayfish Neuromuscular Junction

Ping He,1 R. Chase Southard,1 Dong Chen,2 S. W. Whiteheart,2 and R. L. Cooper1

 1T. H. Morgan School of Biological Science, University of Kentucky, Lexington 40506-0225; and  2Department of Biochemistry, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0084

He, Ping, R. Chase Southard, Dong Chen, S. W. Whiteheart, and R. L. Cooper. Role of alpha -SNAP in Promoting Efficient Neurotransmission at the Crayfish Neuromuscular Junction. J. Neurophysiol. 82: 3406-3416, 1999. In this manuscript, we address the role of the soluble N-ethylmaleimide sensitive factor attachment protein (alpha -SNAP) in synaptic transmission at the neuromuscular junction of the crayfish opener muscle. Immunochemcial methods confirm the presence of alpha -SNAP in these preparations and show that it is concentrated in the synaptic areas. Microinjection and electrophysiological studies show that alpha -SNAP causes an increase in neurotransmitter release yet does not significantly affect the kinetics. More specific quantal analysis, using focal, macropatch, synaptic current recordings, shows that alpha -SNAP increases transmitter release by increasing the probability of exocytosis but not the number of potential release sites. These data demonstrate that the role of alpha -SNAP is to increase the efficiency of neurotransmission by increasing the probability that a stimulus will result in neurotransmitter release. What this suggests is that alpha -SNAP is critical for the formation and maintenance of a "ready release" pool of synaptic vesicles.




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