| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Neurophysiology Vol. 83 No. 1 January 2000, pp. 501-512
Copyright ©2000 by the American Physiological Society
1Department of Anatomy and Neurobiology and Program in Neuroscience, University of Maryland, Baltimore, Maryland 21201; and 2Section of Neurobiology and 3Department of Neurosurgery, Yale University, New Haven, Connecticut 06520
Zufall, Frank,
Trese Leinders-Zufall, and
Charles A. Greer.
Amplification of Odor-Induced Ca2+ Transients by
Store-Operated Ca2+ Release and Its Role in Olfactory
Signal Transduction. J. Neurophysiol. 83: 501-512, 2000. A critical role of Ca2+ in
vertebrate olfactory receptor neurons (ORNs) is to couple odor-induced
excitation to intracellular feedback pathways that are responsible for
the regulation of the sensitivity of the sense of smell, but the role
of intracellular Ca2+ stores in this process remains
unclear. Using confocal Ca2+ imaging and perforated patch
recording, we show that salamander ORNs contain a releasable pool of
Ca2+ that can be discharged at rest by the SERCA inhibitor
thapsigargin and the ryanodine receptor agonist caffeine. The
Ca2+ stores are spatially restricted; emptying produces
compartmentalized Ca2+ release and capacitative-like
Ca2+ entry in the dendrite and soma but not in the cilia,
the site of odor transduction. We deplete the stores to show that odor stimulation causes store-dependent Ca2+ mobilization. This
odor-induced Ca2+ release does not seem to be necessary for
generation of an immediate electrophysiological response, nor does it
contribute significantly to the Ca2+ transients in the
olfactory cilia. Rather, it is important for amplifying the magnitude
and duration of Ca2+ transients in the dendrite and soma
and is thus necessary for the spread of an odor-induced
Ca2+ wave from the cilia to the soma. We show that this
amplification process depends on Ca2+-induced
Ca2+ release. The results indicate that stimulation of ORNs
with odorants can produce Ca2+ mobilization from
intracellular stores without an immediate effect on the receptor
potential. Odor-induced, store-dependent Ca2+ mobilization
may be part of a feedback pathway by which information is transferred
from the distal dendrite of an ORN to its soma.
This article has been cited by other articles:
|
|
 |
|
  B.-J. Lin, T.-W. Chen, and D. Schild Cell type-specific relationships between spiking and [Ca2+]i in neurons of the Xenopus tadpole olfactory bulb J. Physiol., July 1, 2007; 582(1): 163 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Pezier, A. Acquistapace, M. Renou, J.-P. Rospars, and P. Lucas Ca2+ Stabilizes the Membrane Potential of Moth Olfactory Receptor Neurons at Rest and Is Essential for Their Fast Repolarization Chem Senses, May 1, 2007; 32(4): 305 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Kachoei, R. J. Knox, D. Uthuza, S. Levy, L. K. Kaczmarek, and N. S. Magoski A Store-Operated Ca2+ Influx Pathway in the Bag Cell Neurons of Aplysia J Neurophysiol, November 1, 2006; 96(5): 2688 - 2698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Rawson Olfactory Loss in Aging Sci. Aging Knowl. Environ., February 8, 2006; 2006(5): pe6 - pe6. [Abstract] [Full Text]
|
|
|
|
|
|
R. Madrid, R. Delgado, and J. Bacigalupo Cyclic AMP Cascade Mediates the Inhibitory Odor Response of Isolated Toad Olfactory Receptor Neurons J Neurophysiol, September 1, 2005; 94(3): 1781 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gomez, F. W. Lischka, M. E. Haskins, and N. E. Rawson Evidence for Multiple Calcium Response Mechanisms in Mammalian Olfactory Receptor Neurons Chem Senses, May 1, 2005; 30(4): 317 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verkhratsky Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons Physiol Rev, January 1, 2005; 85(1): 201 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ismailov, D. Kalikulov, T. Inoue, and M. J. Friedlander The Kinetic Profile of Intracellular Calcium Predicts Long-Term Potentiation and Long-Term Depression J. Neurosci., November 3, 2004; 24(44): 9847 - 9861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Hegg and M. T. Lucero Dopamine Reduces Odor- and Elevated-K+-Induced Calcium Responses in Mouse Olfactory Receptor Neurons In Situ J Neurophysiol, April 1, 2004; 91(4): 1492 - 1499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Reisert, P. J. Bauer, K.-W. Yau, and S. Frings The Ca-activated Cl Channel and its Control in Rat Olfactory Receptor Neurons J. Gen. Physiol., August 25, 2003; 122(3): 349 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hamana, J. Hirono, M. Kizumi, and T. Sato Sensitivity-dependent Hierarchical Receptor Codes for Odors Chem Senses, February 1, 2003; 28(2): 87 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Spehr, H. Hatt, and C. H. Wetzel Arachidonic Acid Plays a Role in Rat Vomeronasal Signal Transduction J. Neurosci., October 1, 2002; 22(19): 8429 - 8437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Lucas and T. Shimahara Voltage- and Calcium-activated Currents in Cultured Olfactory Receptor Neurons of Male Mamestra brassicae (Lepidoptera) Chem Senses, September 1, 2002; 27(7): 599 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Bozza, P. Feinstein, C. Zheng, and P. Mombaerts Odorant Receptor Expression Defines Functional Units in the Mouse Olfactory System J. Neurosci., April 15, 2002; 22(8): 3033 - 3043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Cinelli, D. Wang, P. Chen, W. Liu, and M. Halpern Calcium Transients in the Garter Snake Vomeronasal Organ J Neurophysiol, March 1, 2002; 87(3): 1449 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zufall and T. Leinders-Zufall The Cellular and Molecular Basis of Odor Adaptation Chem Senses, August 1, 2000; 25(4): 473 - 481. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
