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J Neurophysiol 83: 1052-1057, 2000;
0022-3077/00 $5.00
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The Journal of Neurophysiology Vol. 83 No. 2 February 2000, pp. 1052-1057
Copyright ©2000 by the American Physiological Society

Mapping of IP3-Mediated Ca2+ Signals in Single Human Neuroblastoma SH-SY5Y Cells: Cell Volume Shaping the Ca2+ Signal

K. van Acker, B. Bautmans, G. Bultynck, K. Maes, A. F. Weidema, P. de Smet, J. B. Parys, H. de Smedt, L. Missiaen, and G. Callewaert

Laboratory of Physiology, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium

van Acker, K., B. Bautmans, G. Bultynck, K. Maes, A. F. Weidema, P. de Smet, J. B. Parys, H. de Smedt, L. Missiaen, and G. Callewaert. Mapping of IP3-Mediated Ca2+ Signals in Single Human Neuroblastoma SH-SY5Y Cells: Cell Volume Shaping the Ca2+ Signal. J. Neurophysiol. 83: 1052-1057, 2000. Fast confocal laser-scanning microscopy was used to study spatiotemporal properties of IP3-mediated Ca2+ release signals in human SH-SY5Y neuroblastoma cells. [Ca2+]i increases were not affected by ryanodine (30 µM) or caffeine (10 mM) and largely insensitive to removal of external Ca2+, indicating predominance of IP3-induced Ca2+ release. Ca2+ signals evoked by high concentration (10 µM) of the muscarinic agonist carbachol appeared as self-propagating waves initiating in cell processes. At low carbachol concentrations (500 nM) Ca2+ changes in most cells displayed striking spatiotemporal heterogeneity. The Ca2+ response in the cell body was delayed and had a smaller amplitude and a slower rise time than that in processes. Ca2+ changes in processes either occurred in a homogeneous manner throughout the whole process or were sometimes confined to hot spots. Regional differences in surface-to-volume ratio appear to be critical clues that determine the spatiotemporal pattern of intracellular Ca2+ release signals.




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