The Journal of Neurophysiology Vol. 83 No. 2 February 2000, pp. 659-670
Copyright ©2000 by the American Physiological Society

Development of Glutamatergic Synaptic Activity in Cultured Spinal Neurons

 ¹Department of Neurology and Paralyzed Veterans of America-Eastern Paralyzed Veterans Association Neuroscience Research Center, Yale University School of Medicine, New Haven 06510; Rehabilitation Research Center, Veterans Affairs Medical Center, West Haven 06516; and  ²Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510

Robert, Antoine, James R. Howe, and Stephen G. Waxman. Development of Glutamatergic Synaptic Activity in Cultured Spinal Neurons. J. Neurophysiol. 83: 659-670, 2000. The development of glutamatergic synapses involves a sequence of events that are still not well understood. We have studied the time course of the development of glutamatergic synapses in cultured spinal neurons by characterizing spontaneous synaptic currents recorded from cells maintained in vitro for different times. At short times in culture (2 days in vitro; DIV2), spontaneous synaptic activity consisted almost solely of N-methyl-D-aspartate (NMDA) receptor (NMDAR) openings. In contrast, older neurons (DIV5 to DIV8) displayed clear -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor (AMPAR)-mediated synaptic currents, while the NMDAR-mediated activity remained small. Between 8 and 14 days in vitro there was a large increase in the density of synaptically activated NMDARs, although there was no significant increase in the density of the NMDAR-mediated current activated by exogenous glutamate. The results indicate that there is a switch in NMDAR targeting from somatic to synaptic regions during the course of the second in vitro week. Finally, our results support the conclusion that the spontaneous synaptic activity displayed in culture depends on ongoing NMDAR-mediated activity, even when the expression of synaptic NMDARs is low.