JN AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 83: 1253-1263, 2000;
0022-3077/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vogalis, F.
Right arrow Articles by Smith, T. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vogalis, F.
Right arrow Articles by Smith, T. K.

The Journal of Neurophysiology Vol. 83 No. 3 March 2000, pp. 1253-1263
Copyright ©2000 by the American Physiological Society

Diverse Ionic Currents and Electrical Activity of Cultured Myenteric Neurons From the Guinea Pig Proximal Colon

Fivos Vogalis, Kirk Hillsley, and Terence K. Smith

Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada 89557

Vogalis, Fivos, Kirk Hillsley, and Terence K. Smith. Diverse Ionic Currents and Electrical Activity of Cultured Myenteric Neurons From the Guinea Pig Proximal Colon. J. Neurophysiol. 83: 1253-1263, 2000. The aim of this study was to perform a patch-clamp analysis of myenteric neurons from the guinea pig proximal colon. Neurons were enzymatically dispersed, cultured for 2-7 days, and recorded from using whole cell patch clamp. The majority of cells fired phasically, whereas about one-quarter of the neurons fired in a tonic manner. Neurons were divided into three types based on the currents activated. The majority of tonically firing neurons lacked an A-type current, but generated a large fast transient outward current that was associated with the rapid repolarizing phase of an action potential. The fast transient outward current was dependent on calcium entry and was blocked by tetraethylammonium. Cells that expressed both an A-type current and a fast transient outward current were mostly phasic. Depolarization of these cells to suprathreshold potentials from less than -60 mV failed to trigger action potentials, or action potentials were only triggered after a delay of >50 ms. However, depolarizations from more positive potentials triggered action potentials with minimal latency. Neurons that expressed neither the A-type current or the fast transient outward current were all phasic. Sixteen percent of neurons were similar to AH/type II neurons in that they generated a prolonged afterhyperpolarization following an action potential. The current underlying the prolonged afterhyperpolarization showed weak inward rectification and had a reversal potential near the potassium equilibrium potential. Thus cultured isolated myenteric neurons of the guinea pig proximal colon retain many of the diverse properties of intact neurons. This preparation is suitable for further biophysical and molecular characterization of channels expressed in colonic myenteric neurons.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
F. Vogalis and J. R. Harvey
Altered Excitability of Intestinal Neurons in Primary Culture Caused by Acute Oxidative Stress
J Neurophysiol, June 1, 2003; 89(6): 3039 - 3050.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. Rugiero, M. Mistry, D. Sage, J. A. Black, S. G. Waxman, M. Crest, N. Clerc, P. Delmas, and M. Gola
Selective Expression of a Persistent Tetrodotoxin-Resistant Na+ Current and NaV1.9 Subunit in Myenteric Sensory Neurons
J. Neurosci., April 1, 2003; 23(7): 2715 - 2725.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. H. Kang, P. Vanden Berghe, and T. K. Smith
Ca2+-activated Cl- current in cultured myenteric neurons from murine proximal colon
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C839 - C847.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M.-T. Liu, S. Rayport, Y. Jiang, D. L. Murphy, and M. D. Gershon
Expression and function of 5-HT3 receptors in the enteric neurons of mice lacking the serotonin transporter
Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1398 - G1411.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
F. Vogalis, J. R Harvey, and J. B Furness
TEA- and apamin-resistant KCa channels in guinea-pig myenteric neurons: slow AHP channels
J. Physiol., January 15, 2002; 538(2): 421 - 433.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
F. Rugiero, M. Gola, W. A A Kunze, J.-C. Reynaud, J. B Furness, and N. Clerc
Analysis of whole-cell currents by patch clamp of guinea-pig myenteric neurones in intact ganglia
J. Physiol., January 15, 2002; 538(2): 447 - 463.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
F. Vogalis, J. B. Furness, and W. A. A. Kunze
Afterhyperpolarization Current in Myenteric Neurons of the Guinea Pig Duodenum
J Neurophysiol, May 1, 2001; 85(5): 1941 - 1951.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Hillsley, J. L. Kenyon, and T. K. Smith
Ryanodine-Sensitive Stores Regulate the Excitability of AH Neurons in the Myenteric Plexus of Guinea-Pig Ileum
J Neurophysiol, December 1, 2000; 84(6): 2777 - 2785.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online