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The Journal of Neurophysiology Vol. 83 No. 5 May 2000, pp. 2626-2638
Copyright ©2000 by the American Physiological Society
Department of Anatomy and Neuroscience Training Program, University of Wisconsin Medical School, Madison, Wisconsin 53706
Hefti, Brenda J. and
Philip H. Smith.
Anatomy, Physiology, and Synaptic Responses of Rat Layer V
Auditory Cortical Cells and Effects of Intracellular GABAA
Blockade. J. Neurophysiol. 83: 2626-2638, 2000. The varied extracortical targets of layer V
make it an important site for cortical processing and output, which may
be regulated by differences in the pyramidal neurons found there. Two
populations of projection neurons, regular spiking (RS) and intrinsic
bursting (IB), have been identified in layer V of some sensory
cortices, and differences in their inhibitory inputs have been
indirectly demonstrated. In this report, IB and RS cells were
identified in rat auditory cortical slices, and differences in
thalamocortical inhibition reaching RS and IB cells were demonstrated
directly using intracellular GABAA blockers.
Thalamocortical synaptic input to RS cells was always a combination of
excitation and both GABAA and GABAB inhibition.
Stimulation seldom triggered a suprathreshold response. IB cell
synaptic responses were mostly excitatory, and stimulation usually
triggered action potentials. This apparent difference was confirmed
directly using intracellular chloride channel blockers. Before
intracellular diffusion, synaptic responses were stable and similar to
control conditions. Subsequently, GABAA was blocked,
revealing a cell's total excitatory input. On GABAA blockade, RS cells responded to synaptic stimulation with large, suprathreshold excitatory events, indicating that excitation, while
always present in these cells, is masked by GABAA. In IB cells that had visible GABAA input, it often masked an
excitatory postsynaptic potential (EPSP) that could lead to additional
suprathreshold events. These findings indicate that IB cells receive
less GABAA-mediated inhibitory input and are able to spike
or burst in response to thalamocortical synaptic stimulation far more
readily than RS cells. Such differences may have implications for the
influence each cell type exerts on its postsynaptic targets.
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