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The Journal of Neurophysiology Vol. 83 No. 6 June 2000, pp. 3519-3524
Copyright ©2000 by the American Physiological Society
Department of Pharmacology and Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030
Xiong, Zhi-Qi and
Janet L. Stringer.
Extracellular pH Responses in CA1 and the Dentate Gyrus During
Electrical Stimulation, Seizure Discharges, and Spreading Depression. J. Neurophysiol. 83: 3519-3524, 2000. Since neuronal excitability is sensitive to changes in
extracellular pH and there is regional diversity in the changes in extracellular pH during neuronal activity, we examined the
activity-dependent extracellular pH changes in the CA1 region and the
dentate gyrus. In vivo, in the CA1 region, recurrent epileptiform
activity induced by stimulus trains, bicuculline, and kainic acid
resulted in biphasic pH shifts, consisting of an initial extracellular
alkalinization followed by a slower acidification. In vitro, stimulus
trains also evoked biphasic pH shifts in the CA1 region. However, in CA1, seizure activity in vitro induced in the absence of synaptic transmission, by perfusing with 0 Ca2+/5 mM K+
medium, was only associated with extracellular acidification. In the
dentate gyrus in vivo, seizure activity induced by stimulation to the
angular bundle or by injection of either bicuculline or kainic acid was
only associated with extracellular acidification. In vitro, stimulus
trains evoked only acidification. In the dentate gyrus in vitro,
recurrent epileptiform activity induced in the absence of synaptic
transmission by perfusion with 0 Ca2+/8 mM K+
medium was associated with extracellular acidification. To test whether
glial cell depolarization plays a role in the regulation of the
extracellular pH, slices were perfused with 1 mM barium. Barium
increased the amplitude of the initial alkalinization in CA1 and caused
the appearance of alkalinization in the dentate gyrus. In both CA1 and
the dentate gyrus in vitro, spreading depression was associated with
biphasic pH shifts. These results demonstrate that activity-dependent
extracellular pH shifts differ between CA1 and dentate gyrus both in
vivo and in vitro. The differences in pH fluctuations with neuronal
activity might be a marker for the basis of the regional differences in
seizure susceptibility between CA1 and the dentate gyrus.
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