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The Journal of Neurophysiology Vol. 84 No. 1 July 2000, pp. 575-580
Copyright ©2000 by the American Physiological Society
RAPID COMMUNICATION
Department of Anatomy and Neurobiology and Program in Neuroscience, University of Maryland, Baltimore, Maryland 21201
Chen, Shan,
Andrew P. Lane,
Roland Bock,
Trese Leinders-Zufall, and
Frank Zufall.
Blocking Adenylyl Cyclase Inhibits Olfactory Generator Currents
Induced by "IP3-Odors". J. Neurophysiol. 84: 575-580, 2000. Vertebrate olfactory receptor
neurons (ORNs) transduce odor stimuli into electrical signals by means
of an adenylyl cyclase/cAMP second messenger cascade, but it remains
widely debated whether this cAMP cascade mediates transduction for all
odorants or only certain odor classes. To address this problem, we have
analyzed the generator currents induced by odors that failed to produce cAMP in previous biochemical assays but instead produced
IP3 ("IP3-odors"). We show that in single
salamander ORNs, sensory responses to "cAMP-odors" and
IP3-odors are not mutually exclusive but coexist in the
same cells. The currents induced by IP3-odors exhibit
identical biophysical properties as those induced by cAMP odors or
direct activation of the cAMP cascade. By disrupting adenylyl cyclase
to block cAMP formation using two potent antagonists of adenylyl
cyclase, SQ22536 and MDL12330A, we show that this molecular step is
necessary for the transduction of both odor classes. To assess whether
these results are also applicable to mammals, we examine the
electrophysiological responses to IP3-odors in intact mouse
main olfactory epithelium (MOE) by recording field potentials. The
results show that inhibition of adenylyl cyclase prevents EOG responses
to both odor classes in mouse MOE, even when "hot spots" with
heightened sensitivity to IP3-odors are examined.
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