The Journal of Neurophysiology Vol. 84 No. 2 August 2000, pp. 927-933
Copyright ©2000 by the American Physiological Society

pH Sensitivity of Non-Synaptic Field Bursts in the Dentate Gyrus

 ¹Department of Neurological Surgery, Northwestern University Medical School, Chicago, Illinois 60611; and  ²Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030

Schweitzer, Jeffrey S., Haiwei Wang, Zhi-Qi Xiong, and Janet L. Stringer. pH Sensitivity of Non-Synaptic Field Bursts in the Dentate Gyrus. J. Neurophysiol. 84: 927-933, 2000. Under conditions of low [Ca²⁺]_o and high [K⁺]_o, the rat dentate granule cell layer in vitro develops recurrent spontaneous prolonged field bursts that resemble an in vivo phenomenon called maximal dentate activation. To understand how pH changes in vivo might affect this phenomenon, the slices were exposed to different extracellular pH environments in vitro. The field bursts were highly sensitive to extracellular pH over the range 7.0-7.6 and were suppressed at low pH and enhanced at high pH. Granule cell resting membrane potential, action potentials, and postsynaptic potentials were not significantly altered by pH changes within the range that suppressed the bursts. The pH sensitivity of the bursts was not altered by pharmacologic blockade of N-methyl-D-aspartate (NMDA), non-NMDA, and GABA_A receptors at concentrations of these agents sufficient to eliminate both spontaneous and evoked synaptic potentials. Gap junction patency is known to be sensitive to pH, and agents that block gap junctions, including octanol, oleamide, and carbenoxolone, blocked the prolonged field bursts in a manner similar to low pH. Perfusion with gap junction blockers or acidic pH suppressed field bursts but did not block spontaneous firing of single and multiple units, including burst firing. These data suggest that the pH sensitivity of seizures and epileptiform phenomena in vivo may be mediated in large part through mechanisms other than suppression of NMDA-mediated or other excitatory synaptic transmission. Alterations in electrotonic coupling via gap junctions, affecting field synchronization, may be one such process.