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J Neurophysiol 84: 1681-1691, 2000;
0022-3077/00 $5.00
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The Journal of Neurophysiology Vol. 84 No. 3 September 2000, pp. 1681-1691
Copyright ©2000 by the American Physiological Society

RAPID COMMUNICATION

Characterization of a Hyperpolarization-Activated Inward Current in Cajal-Retzius Cells in Rat Neonatal Neocortex

Werner Kilb and Heiko J. Luhmann

Institut für Neurophysiologie, Heinrich-Heine-Universität Düsseldorf, D-40001 Düsseldorf, Germany

Kilb, Werner and Heiko J. Luhmann. Characterization of a Hyperpolarization-Activated Inward Current in Cajal-Retzius Cells in Rat Neonatal Neocortex. J. Neurophysiol. 84: 1681-1691, 2000. Cajal-Retzius cells are among the first neurons appearing during corticogenesis and play an important role in the establishment of cortical lamination. To characterize the hyperpolarization-activated inward current (Ih) and to investigate whether Ih contributes to the relatively positive resting membrane potential (RMP) of these cells, we analyzed the properties of Ih in visually identified Cajal-Retzius cells in cortical slices from neonatal rats using the whole cell patch-clamp technique. Membrane hyperpolarization to -90 mV activated a prominent inward current that was inhibited by 1 mM Cs+ and was insensitive to 1 mM Ba2+. The activation time constant for Ih was strongly voltage dependent. In Na+-free solution, Ih was reduced, indicating a contribution of Na+. An analysis of the tail currents revealed a reversal potential of -45.2 mV, corresponding to a permeability coefficient (pNa+/pK+) of 0.13. While an increase in the extracellular K+ concentration ([K+]e) enhances Ih, it was reduced by a [K+]e decrease. This [K+]e dependence could not be explained by an effect on the electromotive force on K+ but suggested an additional extracellular binding site for K+ with an apparent dissociation constant of 7.2 mM. Complete Cl- substitution by Br-, I-, or NO3- had no significant effect on Ih, whereas a complete Cl- substitution by the organic compounds methylsulfate, isethionate, or gluconate reduced Ih by ~40%. The Ih reduction observed in gluconate could be abolished by the addition of Cl-. The analysis of the [Cl-]e dependence of Ih revealed a dissociation constant of 9.8 mM and a Hill-coefficient of 2.5, while the assumption of a gluconate-dependent Ih reduction required an unreasonably high Hill-coefficient >20. An internal perfusion with the lidocaine derivative lidocaine N-ethyl bromide blocks Ih within 1 min after establishment of the whole cell configuration. An inhibition of Ih by 1 mM Cs+ was without an effect on RMP, action potential amplitude, threshold, width, or afterhyperpolarization. We conclude from these results that Cajal-Retzius cells express a prominent Ih with characteristic properties that does not contribute to the RMP.




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