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J Neurophysiol 84: 2171-2174, 2000;
0022-3077/00 $5.00
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The Journal of Neurophysiology Vol. 84 No. 4 October 2000, pp. 2171-2174
Copyright ©2000 by the American Physiological Society

RAPID COMMUNICATION

In Vivo Patch-Clamp Analysis of IPSCs Evoked in Rat Substantia Gelatinosa Neurons by Cutaneous Mechanical Stimulation

Keita Narikawa,1,2 Hidemasa Furue,1 Eiichi Kumamoto,1 and Megumu Yoshimura1

 1Department of Physiology and  2Department of Otolaryngology-Head and Neck Surgery, Saga Medical School, Saga 849-8501, Japan

Narikawa, Keita, Hidemasa Furue, Eiichi Kumamoto, and Megumu Yoshimura. In Vivo Patch-Clamp Analysis of IPSCs Evoked in Rat Substantia Gelatinosa Neurons by Cutaneous Mechanical Stimulation. J. Neurophysiol. 84: 2171-2174, 2000. To know a functional role of inhibitory synaptic responses in transmitting noxious and innoxious information from the periphery to the rat spinal dorsal horn, we examined inhibitory postsynaptic currents (IPSCs) elicited in substantia gelatinosa (SG) neurons by mechanical stimuli applied to the skin using the newly developed in vivo patch-clamp technique. In the majority (80%) of SG neurons examined, a brush stimulus applied to the ipsilateral hind limb produced a barrage of IPSCs that persisted during the stimulus, while a pinch stimulus evoked IPSCs only at its beginning and end. The pinch-evoked IPSCs may have been caused by a touch that occurs at the on/off time of the pinch. The evoked IPSCs were blocked by either a glycine-receptor antagonist, strychnine (4 µM), or a GABAA-receptor antagonist, bicuculline (20 µM). All SG neurons examined received inhibitory inputs from a wide area throughout the thigh and lower leg. When IPSCs were examined together with excitatory postsynaptic currents (EPSCs) in the same neurons, a brush evoked a persistent activity of both IPSCs and EPSCs during the stimulus while a pinch evoked such an activity of EPSCs but not IPSCs. It is suggested that innoxious mechanical stimuli activate a GABAergic or glycinergic circuitry in the spinal dorsal horn. This inhibitory transmission may play an important role in the modulation of noxious information in the SG.




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