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The Journal of Neurophysiology Vol. 84 No. 5 November 2000, pp. 2365-2379
Copyright ©2000 by the American Physiological Society
1Department of Physiological Sciences, College of Veterinary Medicine, University of Florida; 2Department of Oral Surgery and Diagnostic Sciences, Division of Neuroscience, University of Florida College of Dentistry; 3Department of Pharmaceutics, University of Florida College of Pharmacy; and 4Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, Florida 32610
Petruska, Jeffrey C.,
Jintana Napaporn,
Richard
D. Johnson,
Jianguo G. Gu, and
Brian Y. Cooper.
Subclassified Acutely Dissociated Cells of Rat DRG:
Histochemistry and Patterns of Capsaicin-, Proton-, and ATP-Activated
Currents. J. Neurophysiol. 84: 2365-2379, 2000. We used a "current signature" method to subclassify acutely
dissociated dorsal root ganglion (DRG) cells into nine subgroups. Cells
subclassified by current signature had uniform properties. The type 1 cell had moderate capsaicin sensitivity (25.9 pA/pF), powerful, slowly
desensitizing (
= 2,300 ms), ATP-activated current (13.3 pA/pF), and small nondesensitizing responses to acidic solutions (5.6 pA/pF). Type 1 cells expressed calcitonin gene-related peptide immunoreactivity (CGRP-IR), manifested a wide action potential (7.3 ms), long duration afterhyperpolarization (57.0 ms), and were IB4
positive. The type 2 cell exhibited large capsaicin activated currents
(134.9 pA/pF) but weak nondesensitizing responses to protons (15.3 pA/pF). Currents activated by ATP and 
-m-ATP (51.7 and 44.6 pA/pF, respectively) had fast desensitization kinetics ( = 214 ms) that were distinct from all other cell types. Type 2 cells were IB4
positive but did not contain either substance P (SP) or CGRP-IR.
Similar to capsaicin-sensitive nociceptors in vivo, the
afterhyperpolarization of the type 2 cell was prolonged (54.7 ms). The
type 3 cell expressed, amiloride-sensitive, rapidly desensitizing
( = 683 ms) proton-activated currents (127.0 pA/pF), and was
insensitive to ATP or capsaicin. The type 3 cell was IB4 negative and
contained neither CGRP nor SP-IR. The afterhyperpolarization (17.5 ms)
suggested nonnociceptive function. The type 4 cell had powerful
ATP-activated currents (17.4 pA/pF) with slow desensitization kinetics
( = 2,813 ms). The afterhyperpolarization was prolonged (46.5 ms), suggesting that this cell type might belong to a
capsaicin-insensitive nociceptor population. The type 4 cell did not
contain peptides. The type 7 cell manifested amiloride-sensitive,
proton-activated currents (45.8 pA/pF) with very fast desensitization
kinetics ( = 255 ms) and was further distinct from the type 3 cell by virtue of a nondesensitizing amiloride-insensitive component
(6.0 pA/pF). Capsaicin and ATP sensitivity were relatively weak (4.3 and 2.9 pA/pF, respectively). Type 7 cells were IB4 positive and contained both SP and CGRP-IR. They exhibited an exceptionally long
afterhyperpolarization (110 ms) that was suggestive of a silent
(mechanically insensitive) nociceptor. We concluded that presorting of
DRG cells by current signatures separated them into internally
homogenous subpopulations that were distinct from other subclassified
cell types.
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