JN Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 84: 2630-2637, 2000;
0022-3077/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, W.-J.
Right arrow Articles by Murakami, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, W.-J.
Right arrow Articles by Murakami, F.

The Journal of Neurophysiology Vol. 84 No. 5 November 2000, pp. 2630-2637
Copyright ©2000 by the American Physiological Society

Characterization of Ca2+ Channels in Rat Subthalamic Nucleus Neurons

Wen-Jie Song,1 Yosuke Baba,2 Takeshi Otsuka,1 and Fujio Murakami2,3

 1Department of Electronic Engineering, Graduate School of Engineering, Osaka University, Suita 565-0871; and  2Division of Biophysical Engineering, Graduate School of Engineering Science and  3Core Research for Evolutional Science and Technology/Murakami Laboratory, Center for Advanced Research Projects, Osaka University, Toyonaka 560-8531, Japan

Song, Wen-Jie, Yosuke Baba, Takeshi Otsuka, and Fujio Murakami. Characterization of Ca2+ Channels in Rat Subthalamic Nucleus Neurons. J. Neurophysiol. 84: 2630-2637, 2000. The subthalamic nucleus (STN) plays a key role in motor control. Although previous studies have suggested that Ca2+ conductances may be involved in regulating the activity of STN neurons, Ca2+ channels in this region have not yet been characterized. We have therefore investigated the subtypes and functional characteristics of Ca2+ conductances in STN neurons, in both acutely isolated and slice preparations. Acutely isolated STN cells were identified by retrograde filling with the fluorescent dye, Fluoro-Gold. In acutely isolated STN neurons, Cd2+-sensitive, depolarization-activated Ba2+ currents were observed in all cells studied. The current-voltage relationship and current kinetics were characteristic of high-voltage-activated Ca2+ channels. The steady-state voltage-dependent activation curves and inactivation curves could both be fitted with a single Boltzmann function. Currents evoked with a prolonged pulse, however, inactivated with multiple time constants, suggesting either the presence of more than one Ca2+ channel subtype or multiple inactivation processes with a single channel type in STN neurons. Experiments using organic Ca2+ channel blockers revealed that on average, 21% of the current was nifedipine sensitive, 52% was sensitive to omega -conotoxin GVIA, 16% was blocked by a high concentration of omega -agatoxin IVA (200 nM), and the remainder of the current (9%) was resistant to the co-application of all blockers. These currents had similar voltage dependencies, but the nifedipine-sensitive current and the resistant current activated at slightly lower voltages. omega -Agatoxin IVA at 20 nM was ineffective in blocking the current. Together, the above results suggest that acutely isolated STN neurons have all subtypes of high-voltage-activated Ca2+ channels except for P-type, but have no low-voltage-activated channels. Although acutely isolated neurons provide a good preparation for whole cell voltage-clamp study, dendritic processes are lost during dissociation. To gain information on Ca2+ channels in dendrites, we thus studied Ca2+ channels of STN neurons in a slice preparation, focusing on low-voltage-activated channels. In current-clamp recordings, a slow spike was always observed following termination of an injected hyperpolarizing current. The slow spike occurred at resting membrane potentials and was sensitive to micromolar concentrations of Ni2+, suggesting that it is a low-threshold Ca2+ spike. Together, our results suggest that STN neurons express low-voltage-activated Ca2+ channels and several high-voltage-activated subtypes. Our results also suggest the possibility that the low-voltage-activated channels have a preferential distribution to the dendritic processes.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
S. W. Johnson
Rebound bursts following inhibition: how dopamine modifies firing pattern in subthalamic neurons
J. Physiol., April 15, 2008; 586(8): 2033 - 2033.
[Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Ramanathan, T. Tkatch, J. F. Atherton, C. J. Wilson, and M. D. Bevan
D2-Like Dopamine Receptors Modulate SKCa Channel Function in Subthalamic Nucleus Neurons Through Inhibition of Cav2.2 Channels
J Neurophysiol, February 1, 2008; 99(2): 442 - 459.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Teagarden, J. F. Atherton, M. D. Bevan, and C. J. Wilson
Accumulation of cytoplasmic calcium, but not apamin-sensitive afterhyperpolarization current, during high frequency firing in rat subthalamic nucleus cells
J. Physiol., February 1, 2008; 586(3): 817 - 833.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. D. Humphries, R. D. Stewart, and K. N. Gurney
A Physiologically Plausible Model of Action Selection and Oscillatory Activity in the Basal Ganglia
J. Neurosci., December 13, 2006; 26(50): 12921 - 12942.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Gillies and D. Willshaw
Membrane Channel Interactions Underlying Rat Subthalamic Projection Neuron Rhythmic and Bursting Activity
J Neurophysiol, April 1, 2006; 95(4): 2352 - 2365.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. I. Kass and I. M. Mintz
Silent plateau potentials, rhythmic bursts, and pacemaker firing: Three patterns of activity that coexist in quadristable subthalamic neurons
PNAS, January 3, 2006; 103(1): 183 - 188.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. Baufreton, Z.-T. Zhu, M. Garret, B. Bioulac, S. W. Johnson, and A. I. Taupignon
Dopamine receptors set the pattern of activity generated in subthalamic neurons
FASEB J, November 1, 2005; 19(13): 1771 - 1777.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Baufreton, J. F. Atherton, D. J. Surmeier, and M. D. Bevan
Enhancement of Excitatory Synaptic Integration by GABAergic Inhibition in the Subthalamic Nucleus
J. Neurosci., September 14, 2005; 25(37): 8505 - 8517.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. F. Atherton and M. D. Bevan
Ionic Mechanisms Underlying Autonomous Action Potential Generation in the Somata and Dendrites of GABAergic Substantia Nigra Pars Reticulata Neurons In Vitro
J. Neurosci., September 7, 2005; 25(36): 8272 - 8281.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Yasoshima, N. Kai, S. Yoshida, S. Shiosaka, Y. Koyama, Y. Kayama, and K. Kobayashi
Subthalamic Neurons Coordinate Basal Ganglia Function through Differential Neural Pathways
J. Neurosci., August 24, 2005; 25(34): 7743 - 7753.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. T. Paz, J.-M. Deniau, and S. Charpier
Rhythmic Bursting in the Cortico-Subthalamo-Pallidal Network during Spontaneous Genetically Determined Spike and Wave Discharges
J. Neurosci., February 23, 2005; 25(8): 2092 - 2101.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. T. H. Do and B. P. Bean
Sodium Currents in Subthalamic Nucleus Neurons From Nav1.6-Null Mice
J Neurophysiol, August 1, 2004; 92(2): 726 - 733.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
T. Otsuka, T. Abe, T. Tsukagawa, and W.-J. Song
Conductance-Based Model of the Voltage-Dependent Generation of a Plateau Potential in Subthalamic Neurons
J Neurophysiol, July 1, 2004; 92(1): 255 - 264.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. J. Wilson, A. Weyrick, D. Terman, N. E. Hallworth, and M. D. Bevan
A Model of Reverse Spike Frequency Adaptation and Repetitive Firing of Subthalamic Nucleus Neurons
J Neurophysiol, May 1, 2004; 91(5): 1963 - 1980.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
C. Hamani, J. A. Saint-Cyr, J. Fraser, M. Kaplitt, and A. M. Lozano
The subthalamic nucleus in the context of movement disorders
Brain, January 1, 2004; 127(1): 4 - 20.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. E. Hallworth, C. J. Wilson, and M. D. Bevan
Apamin-Sensitive Small Conductance Calcium-Activated Potassium Channels, through their Selective Coupling to Voltage-Gated Calcium Channels, Are Critical Determinants of the Precision, Pace, and Pattern of Action Potential Generation in Rat Subthalamic Nucleus Neurons In Vitro
J. Neurosci., August 20, 2003; 23(20): 7525 - 7542.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Hattori, F. Murakami, and W.-J. Song
Quantitative Relationship Between Kv4.2 mRNA and A-Type K+ Current in Rat Striatal Cholinergic Interneurons During Development
J Neurophysiol, July 1, 2003; 90(1): 175 - 183.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Baufreton, M. Garret, A. Rivera, A. de la Calle, F. Gonon, B. Dufy, B. Bioulac, and A. Taupignon
D5 (Not D1) Dopamine Receptors Potentiate Burst-Firing in Neurons of the Subthalamic Nucleus by Modulating an L-Type Calcium Conductance
J. Neurosci., February 1, 2003; 23(3): 816 - 825.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D. Terman, J. E. Rubin, A. C. Yew, and C. J. Wilson
Activity Patterns in a Model for the Subthalamopallidal Network of the Basal Ganglia
J. Neurosci., April 1, 2002; 22(7): 2963 - 2976.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. D. Bevan, P. J. Magill, N. E. Hallworth, J. P. Bolam, and C. J. Wilson
Regulation of the Timing and Pattern of Action Potential Generation in Rat Subthalamic Neurons In Vitro by GABA-A IPSPs
J Neurophysiol, March 1, 2002; 87(3): 1348 - 1362.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
T. Otsuka, F. Murakami, and W.-J. Song
Excitatory Postsynaptic Potentials Trigger a Plateau Potential in Rat Subthalamic Neurons at Hyperpolarized States
J Neurophysiol, October 1, 2001; 86(4): 1816 - 1825.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online