JN AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 85: 995-997, 2001;
0022-3077/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karadsheh, M. F.
Right arrow Articles by Delpire, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karadsheh, M. F.
Right arrow Articles by Delpire, E.

The Journal of Neurophysiology Vol. 85 No. 2 February 2001, pp. 995-997
Copyright ©2001 by the American Physiological Society

RAPID COMMUNICATION

Neuronal Restrictive Silencing Element Is Found in the KCC2 Gene: Molecular Basis for KCC2-Specific Expression in Neurons

Michael F. Karadsheh and E. Delpire

Departments of Anesthesiology and Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee 37232-2520

Karadsheh, Michael F. and E. Delpire. Neuronal Restrictive Silencing Element Is Found in the KCC2 Gene: Molecular Basis for KCC2-Specific Expression in Neurons. J. Neurophysiol. 85: 995-997, 2001. KCC2 is one of four known isoforms of the K-Cl cotransporter with an expression pattern restricted to neurons. It mediates efflux of Cl- across neuronal membranes and plays an important role in GABAergic and glycinergic neurotransmission. To understand the molecular basis for neuronal specificity of KCC2 expression, we isolated and sequenced portions of the KCC2 gene, including some of its 5' flanking (control) region. We found a 21-bp sequence, within intron 1, that shares 80% homology to the consensus site for neuronal-restrictive silencing factor binding. We demonstrated that this specific sequence of the KCC2 gene promotes transcriptional regulation by showing that nuclear proteins isolated from a mouse neural progenitor cell line interact with this 21-bp element and by establishing that this element silences reporter gene expression in nonneuronal cells.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. Uvarov, A. Ludwig, M. Markkanen, P. Pruunsild, K. Kaila, E. Delpire, T. Timmusk, C. Rivera, and M. S. Airaksinen
A Novel N-terminal Isoform of the Neuron-specific K-Cl Cotransporter KCC2
J. Biol. Chem., October 19, 2007; 282(42): 30570 - 30576.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. H. C. Lee, J. A. Walker, J. R. Williams, R. J. Goodier, J. A. Payne, and S. J. Moss
Direct Protein Kinase C-dependent Phosphorylation Regulates the Cell Surface Stability and Activity of the Potassium Chloride Cotransporter KCC2
J. Biol. Chem., October 12, 2007; 282(41): 29777 - 29784.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Gamba
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
Physiol Rev, April 1, 2005; 85(2): 423 - 493.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Zhu, D. Lovinger, and E. Delpire
Cortical Neurons Lacking KCC2 Expression Show Impaired Regulation of Intracellular Chloride
J Neurophysiol, March 1, 2005; 93(3): 1557 - 1568.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Brauer, E. Frei, L. Claes, S. Grissmer, and H. Jager
Influence of K-Cl cotransporter activity on activation of volume-sensitive Cl- channels in human osteoblasts
Am J Physiol Cell Physiol, July 1, 2003; 285(1): C22 - C30.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online