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The Journal of Neurophysiology Vol. 86 No. 1 July 2001, pp. 104-112
Copyright ©2001 by the American Physiological Society
Department of Organismal Biology and Anatomy, Committee on Neurobiology, The University of Chicago, Chicago, Illinois 60637
Thoby-Brisson, Muriel and
Jan-Marino Ramirez.
Identification of Two Types of Inspiratory Pacemaker Neurons in
the Isolated Respiratory Neural Network of Mice. J. Neurophysiol. 86: 104-112, 2001. In the respiratory
network of mice, we characterized with the whole cell patch-clamp
technique pacemaker properties in neurons discharging in phase with
inspiration. The respiratory network was isolated in a transverse brain
stem slice containing the pre-Bötzinger complex (PBC), the
presumed site for respiratory rhythm generation. After blockade of
respiratory network activity with 6-cyano-7-nitroquinoxalene-2,3-dione (CNQX), 18 of 52 inspiratory neurons exhibited endogenous pacemaker activity, which was voltage dependent, could be reset by brief current
injections and could be entrained by repetitive stimuli. In the
pacemaker group (n = 18), eight neurons generated brief bursts (0.43 ± 0.03 s) at a relatively high frequency
(1.05 ± 0.12 Hz) in CNQX. These bursts resembled the bursts that
these neurons generated in the intact network during the interval
between two inspiratory bursts. Cadmium (200 µM) altered but did not
eliminate this bursting activity, while 0.5 µM tetrodotoxin
suppressed bursting activity. Another set of pacemaker neurons (10 of
18) generated in CNQX longer bursts (1.57 ± 0.07 s) at a
lower frequency (0.35 ± 0.01 Hz). These bursts resembled the
inspiratory bursts generated in the intact network in phase with the
population activity. This bursting activity was blocked by 50-100 µM
cadmium or 0.5 µM tetrodotoxin. We conclude that the respiratory
neural network contains pacemaker neurons with two types of bursting
properties. The two types of pacemaker activities might have different
functions within the respiratory network.
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